Selective separation of bovine hemoglobin using magnetic mesoporous rare-earth silicate microspheres

Wang, Jundong; Tan, Siyuan; Liang, Qionglin; Guan, Huiyuan; Han, Qiang; Ding, Mingyu

doi:10.1016/j.talanta.2019.06.069

Cited by 29 publications

(8 citation statements)

References 45 publications

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“…Zhang et al [ 49 ] prepared magnetic carbon nanotubes with a hierarchical copper silicate nanostructure for BHb adsorption, with the maximum BHb adsorption capacity being 302 mg/g. Wang et al [ 50 ] reported that magnetic mesoporous ytterbium silicate microspheres achieved a BHb adsorption capacity of 304 mg/g. Compared to these materials, the CB-modified PVA nanofiber fabric has a distinct advantage in adsorption capacity.…”

Section: Resultsmentioning

confidence: 99%

Selective Adsorption and Separation of Proteins by Ligand-Modified Nanofiber Fabric

Liu

Mukai

2021

Polymers

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Electrospun polyvinyl alcohol (PVA) nanofiber fabric was modified by Cibacron Blue F3GA (CB) to enhance the affinity of the fabric. Batch experiments were performed to study the nanofiber fabric’s bovine hemoglobin (BHb) adsorption capacity at different protein concentrations before and after modification. The maximum BHb adsorption capacity of the modified nanofiber fabric was 686 mg/g, which was much larger than the 58 mg/g of the original fabric. After that, the effect of feed concentration and permeation rate on the dynamic adsorption behaviors for BHb of the nanofiber fabric was investigated. The pH impact on BHb and bovine serum albumin (BSA) adsorption was examined by static adsorption experiments of single protein solutions. The selective separation experiments of the BHb–BSA binary solution were carried out at the optimal pH value, and a high selectivity factor of 5.45 for BHb was achieved. Finally, the reusability of the nanofiber fabric was examined using three adsorption–elution cycle tests. This research demonstrated the potential of the CB-modified PVA nanofiber fabric in protein adsorption and selective separation.

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Section: Resultsmentioning

confidence: 99%

Selective Adsorption and Separation of Proteins by Ligand-Modified Nanofiber Fabric

Liu

Mukai

2021

Polymers

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“…To investigate the effect of incubation time on the adsorption mechanism, the kinetics of three different proteins (including target protein BHB) in magnetic hollow nanocages at pH 7.4 were investigated (Figure b). It can be noticed that the binding capacity of the resultant nanocages was time-dependent, and the adsorption amount would gradually increase with the increase of the incubation time within the range of 0–2.0 h . After the incubation time exceeded 2.0 h, the kinetics would reach an equilibrium plateau.…”

Section: Resultsmentioning

confidence: 99%

Designed Fabrication of Polymer-Mediated MOF-Derived Magnetic Hollow Carbon Nanocages for Specific Isolation of Bovine Hemoglobin

Tan

Long

Han

et al. 2020

ACS Biomater. Sci. Eng.

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It is highly required to develop well-designed separation materials for the specific isolation of certain proteins in proteomic research. Herein, the new type of metal–organic framework (MOF)-derived polymer-mediated magnetic hollow nanocages was fabricated via stress-induced orientation contraction, which was further applied for specific enrichment of proteins. The core–shell nanocomposites comprised of polymer-mediated ZIF-67 cores and polydopamine (PDA) shells, after annealing, generated magnetic hollow carbon nanocages with hierarchical pores and structures. Particularly, the magnetic carbonized PDA@F127/ZIF-67 hollow nanocages exhibited a remarkable adsorption capacity toward bovine hemoglobin (BHB) up to 834.3 mg g–1, which was significantly greater than that of the directed carbonized ZIF-67 nanoparticles. The results also exhibited the notable specificity of the obtained nanocages on complex biosamples, including intact mixed proteins and fetal calf serum. The hierarchically hollow porous structure greatly improves the specific surface area and reduces the mass transfer resistance, leading to enhanced high adsorption for target protein BHB. This novel method will be promising for the applications in purification and enrichment of biomacromolecules for complex biosamples, which successfully solve the problem of low adsorption efficiency and tedious separating process of the previous MOF-derived materials.

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“…Bovine hemoglobin (BHb) has a high similarity with Hb, with around 90% match of amino acid sequence, thus acting as an impeccable substitute of Hb protein. 3,4 Accurate monitoring of a high-quality biological macromolecule like BHb is challenging owing to its complex structure and uncontrollable stability, which emphasizes the need for the development of an efficient method for the separation and enrichment of BHb in complex biological samples. 5 Currently, a plethora of enrichment strategies have been developed for the separation of highly-abundant proteins, including lectin affinity, hydrophilic, and electrostatic interactions, etc.…”

Section: Introductionmentioning

confidence: 99%

Preparation of carbon-based metal organic framework-modified molecularly imprinted polymers for selective recognition of bovine hemoglobin in biological samples

et al. 2022

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Selective separation of bovine hemoglobin using magnetic mesoporous rare-earth silicate microspheres

Cited by 29 publications

References 45 publications

Selective Adsorption and Separation of Proteins by Ligand-Modified Nanofiber Fabric

Selective Adsorption and Separation of Proteins by Ligand-Modified Nanofiber Fabric

Designed Fabrication of Polymer-Mediated MOF-Derived Magnetic Hollow Carbon Nanocages for Specific Isolation of Bovine Hemoglobin

Preparation of carbon-based metal organic framework-modified molecularly imprinted polymers for selective recognition of bovine hemoglobin in biological samples

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