Selective visualization of hypochlorite and its fluctuation in cancer cells by a mitochondria-targeting ratiometric fluorescent probe

Hu, Qiao; Qin, Caiqin; Huang, Lei; Wang, Hongying; Liu, Qiang; Zeng, Lintao

doi:10.1016/j.dyepig.2017.10.002

Cited by 108 publications

(14 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hu et al developed a far-red-emitting ratiometric fluorescent probe to detect hypochlorite (ClO − ) in cancer cells (24) [57]. While most H 2 O 2 -detecting probes utilize a reactive boronate ester, probes designed to detect ClO − have an ethylene group that undergoes rapid oxidation to generate an aldehyde.…”

Section: Mitochondrion-targeted Probesmentioning

confidence: 99%

Recent Advances in Organelle-Targeted Fluorescent Probes

et al. 2021

View full text Add to dashboard Cite

Recent advances in fluorescence imaging techniques and super-resolution microscopy have extended the applications of fluorescent probes in studying various cellular processes at the molecular level. Specifically, organelle-targeted probes have been commonly used to detect cellular metabolites and transient chemical messengers with high precision and have become invaluable tools to study biochemical pathways. Moreover, several recent studies reported various labeling strategies and novel chemical scaffolds to enhance target specificity and responsiveness. In this review, we will survey the most recent reports of organelle-targeted fluorescent probes and assess their general strategies and structural features on the basis of their target organelles. We will discuss the advantages of the currently used probes and the potential challenges in their application as well as future directions.

show abstract

Section: Mitochondrion-targeted Probesmentioning

confidence: 99%

Recent Advances in Organelle-Targeted Fluorescent Probes

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Fluorescence imaging is a promising alternative technique for the detection of HClO in early-stage RA because of its high sensitivity, spatiotemporal resolution, real-time detection, and easy manipulation. − To date, a wide variety of fluorescent probes have been explored for imaging HClO in vitro and in vivo, including off–on, − ratiometric, − ON–OFF, − and two-photo − type probes based on HOCl-mediated oxidation reactions of chalcogenide, hydrazine, hydrazone, Schiff base, double bonds, and so on. Although great achievements have been made, most of these fluorescent probes display low sensitivity, poor water solubility, slow response time, and shallow tissue penetration depth, which largely limited their further applications in the early detection of deep-seated RA in vivo.…”

Section: Introductionmentioning

confidence: 99%

A Fast-Responsive OFF–ON Near-Infrared-II Fluorescent Probe for In Vivo Detection of Hypochlorous Acid in Rheumatoid Arthritis

et al. 2021

View full text Add to dashboard Cite

Rheumatoid arthritis (RA) is a common chronic autoimmune inflammatory disease, and its etiology is closely related to the overproduction of hypochlorous acid (HClO). However, early detection of RA using an activatable near-infrared-II (NIR-II, 1000−1700 nm) fluorescent probe remains challenging. Herein, we first report an "OFF− ON" NIR-II fluorescent probe named PTA (phenothiazine triphenylamine) for imaging HClO in deep-seated early RA. Electron-rich phenothiazine in the core of PTA was utilized as both an HClOrecognition moiety and a precursor of electron acceptors, displaying a typical donor−acceptor−donor structure with excellent NIR-II emission at 936/1237 nm once reacted with HClO. The probe PTA exhibited good water solubility, high photostability, and rapid response capability toward HClO within 30 s. Moreover, it was able to sensitively and specifically detect exogenous and endogenous HClO in living cells in both visible and NIR-II windows. Notably, PTA enabled the sensitive and rapid visualization of HClO generation in an inflammatory RA mouse model, showing a 4.3-fold higher NIR-II fluorescence intensity than that in normal hindlimb joints. These results demonstrate that PTA holds great promise as a robust platform for diagnosis of HOCl-mediated inflammatory disorders.

show abstract

“…Recently, Zeng et al developed a coumarin-based chemosensor for discriminative detection of hydrazine and bisulfate via hydrazine-induced ethyl cyanoacetate cleavage and bisulfateinvolved Michael addition [17]. Likewise, several fluorescent sensors for visual detection of HOCl/ClO − have been explored on the basis of the ClO − -mediated oxidation reaction of various functional groups such as ether [18], p-methoxyphenol [19], thioether [20,21], oxime [22][23][24], hydroxamic acid [25] and C_C double bond [26,27]. Although the above-mentioned progresses have been made, until now, two analytes ClO − and N 2 H 4 hardly be distinguishingly determined by using a simple, effective and visible chemosensor.…”

Section: Introductionmentioning

confidence: 99%

“…It is disclosed that integration of two different reaction/binding sites into a single fluorescent probe could be an efficient approach to achieve discriminative detection of two analytes [17,[26][27][28]. In this study, we designed a dual-analytes responsive fluorescent probe PTMQ for the distinguishing detection of ClO − and N 2 H 4 .…”

Section: Introductionmentioning

confidence: 99%