Selectivity of Ethanol Extract of Parijoto (Medinilla speciosa) Fruit in HepG2, Widr, 4T1, and Vero cell lines

Abstract

Background: Parijoto, one of the melastomaceae family, has been known to have cytotoxic activity in some cancer cell lines, such as HeLa, MCF-7, and T47D. Aims: We aim to know about the selectivity of ethanol extract of Parijoto fruit in cell line HepG2, WiDr, 4T1, and Vero. Cytotoxic was determined by MTT assay. Method: Extract was added in three serial concentration three serial concentrations (125 µg/mL–500 µg/mL), while the positive control doxorubicin gives in 2,5 µg/mL – 20 µg/mL for cancer cell and 40 µg/mL -100 µg/mL for Vero cell. Results: Results showed that ethanol extract of parijoto fruit gave low activity in HepG2 and Vero cell (IC50: 250 µg/mL) and moderate activity in WiDr and 4T1 (IC50: 81,58 µg/mL and 158,72 µg/mL). Conclusion: The highest selectivity index is given in WiDr cell (SI> 3) means that the ethanol extract of parijoto fruit is a promising cytotoxic agent for colorectal cancer therapy.

REVIEW ARTICLE: AKTIVITAS FARMAKOLOGI TANAMAN PARIJOTO (Medinilla speciosa)

“…Ekstrak metanol buah parijoto dapat memberikan efek sitotoksik terhadap sel HeLa dengan IC50 sebesar 209,6 µg/mL [24]. Sedangkan pada ekstrak etanol buah parijoto memberikan aktivitas rendah pada sel HepG2 dan vero yaitu IC50 250 µg/mL dan aktivitas sedang pada sel WiDr dan 4T1 yaitu IC50 81,58 µg/mL dan 158,72 µg/mL [25]. Aktivitas sitotoksik pada fraksi buah parijoto menggunakan pelarut etil asetat terhadap sel kanker serviks HeLa memiliki nilai IC50 sebesar 95,48 µg/mL [26] Kandungan buah parijoto yang mempunyai aktivitas sebagai antikanker adalah kuersetin, flavonoid dan saponin.…”