Selenium compounds in selenium-enriched cabbage

Abstract

For the study, cabbage (Brassica oleracea var. capitata L.) and red cabbage (Brassica oleracea var. capitata L. f. rubra) were treated with Na selenate. Cabbage was foliarly sprayed twice with 20 mg Se(VI) L–1, while red cabbage was fertilized twice with 0.5 mg Se(VI) L–1. Despite the high dose of Se, no toxic effects were observed on cabbage plants. The total Se concentration in cabbage leaves was 4.80 ± 0.25 μg Se g–1 (DM) and in red cabbage 0.96 ± 0.04 μg Se g–1 (DM). Soluble Se compounds were extracted from parts of cabbage with protease XIV, resulting in 49 % of soluble Se from roots, 59 % from leaves, and 65 % from stems. In red cabbage, the corresponding figures were 28 % of soluble Se in roots, 31 % in stems, and 43 % in leaves. Se species were determined in the enzymatic extracts using ion exchange high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). The main Se species found in all parts of cabbage and red cabbage was selenomethionine (SeMet), which in roots represented 94 and 55 % of the soluble Se content in cabbage and red cabbage, respectively. In stems and leaves of cabbage, SeMet represented only 23 % of the soluble Se content. In stems of red cabbage SeMet represented 80 % and in leaves 41 % of the soluble Se content. We observed that traces of Se(VI) were present in upper parts of both plants.

The Physiology and Biochemical Tolerance of Cabbage to Se (VI) Addition to the Soil and by Foliar Spraying

“…Some other soluble Se species were present, which could be not identified with the method used [high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS)]. It was concluded that foliarly added Se (VI) was mainly transformed to organic forms of Se in cabbage (Mechora et al, 2012).…”

Selenium uptake by edible plants from enriched peat

“…This finding is attributed to the high fortification in peat. A high percentage of inorganic species were converted to SeMet, which was the major organic species in the enzymatic plant extracts as in other studies (Polatajco et al, 2006;Mazej et al, 2007;Mechora et al, 2012). The occurrence of SeCys 2 in enzymatic extracts has been also studied and the results showed that the concentration of this species was lower than its detection limit (0.03 mg kg -1 ).…”

“…Several dominant species have been identified in plant foods. The main one is SeMet, and its behaviour has been examined widely due to interest in its biological activity (Reilly, 2006;Mechora et al, 2012;Mazej et al, 2007). Here we addressed Se speciation in vegetables.…”

Regulation of Selenium/Sulfur Interactions to Enhance Chemopreventive Effects: Lessons to Learn from Brassicaceae

“…In this study, Se soluble compounds were extracted from cabbage using protease XIV and identified by ion exchange high-performance liquid chromatography inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). The authors found that 49%, 59%, and 65% of soluble Se compounds were from roots, leaves, and stems, respectively, whereas in red cabbage 28%, 31%, and 43% of soluble Se were detected in roots, stems, and leaves, respectively [ 87 ]. The insoluble Se was 31–53% and the authors suggested these amounts might be a part of a detoxification mechanism.…”