Selenium Supplementation Restores the Antioxidative Capacity and Prevents Cell Damage in Bone Marrow Stromal Cells In Vitro

Ebert, Regina; Ulmer, Matthias; Zeck, Sabine; Meissner‐Weigl, Jutta; Schneider, Doris; Stopper, Helga; Schupp, Nicole; Kassem, Moustapha; Jakob, Franz

doi:10.1634/stemcells.2005-0117

Cited by 174 publications

(125 citation statements)

References 72 publications

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“…Similarly as in our study, 48-hour-exposure to selenium nanoparticles showed no significant influence on SOD in intestinal epithelial cells of crucian carp [22]. On the other hand, treatment with 100 nM sodium selenite caused increase in SOD activity in telomerase-immortalized human mesenchymal stem cell line [18]. In another interesting study the comparison of three different forms of selenium (methylseleninic acid, methylselenocysteine and Se yeast) was performed on ERpositive MCF-7 and triple-negative MDA-MB 231 human breast cancer cell lines.…”

Section: Discussionsupporting

confidence: 84%

“…Sodium selenite (1μM in the culture media, 11 days) significantly enhanced GPx in human hepatoma Hep G2 cells [17]. Sodium selenite (100 nM) supplementation caused significant increase in GPx activity in bone marrow stromal cells as well as in telometase-immortalized human mesenchymal stem cell line [18]. According to Barayuga et al sodium selenite (10 or 100 nM for a week) was found to increase GPx1 and GPx4 isoforms in SH-SY5Y neuronal cells in dose-dependent way [5].…”

Section: Discussionmentioning

confidence: 97%

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Lithium as a prooxidant? A possible protective role of selenium – in vitro study

Musik

Kiełczykowska

Rajtar

et al. 2017

Ann Agric Environ Med.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 97%

Lithium as a prooxidant? A possible protective role of selenium – in vitro study

Musik

Kiełczykowska

Rajtar

et al. 2017

Ann Agric Environ Med.

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“…Chronic treatment of N-acetyl cysteine or supplementation with selenium can reduce senescence markers through reduction of ROS levels [35,36]. APE1/Ref-1 can also decrease intracellular ROS levels [16].…”

Section: Overexpression Of Ape1/ref-1 Decreases Superoxide Generationmentioning

confidence: 99%

Downregulation of APE1/Ref-1 Is Involved in the Senescence of Mesenchymal Stem Cells

et al. 2009

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The senescence of human mesenchymal stem cells (hMSCs) causes disruption of tissue and organ maintenance, and is thus an obstacle to stem cell-based therapies for disease. Although some researchers have studied changes in the characteristics of hMSCs (decreases in differentiation ability and self-renewal), comparing young and old ages, the mechanisms of stem cell senescence have not yet been defined. In this study, we developed a growth curve for human bone marrow derived MSCs (hBMSCs) which changes into a hyperbolic state after passage number 7. Senescence associated b-galactosidase (SA b-gal) staining of hBMSCs showed 10% in passage 9 and 45% in passage 11. We detected an increase in endogenous superoxide levels during senescence that correlated with senescence markers (SA b-gal, hyperbolic growth curve). Interestingly, even though endogenous superoxide increased in a replicative senescence model, the expression of APE1/Ref-1, which is sensitive to intracellular redox state, decreased. These effects were confirmed in a stressinduced senescence model by exogenous treatment with H 2 O 2 . This change is related to the p53 activity that negatively regulates APE1/Ref-1. p21 expression levels, which represent p53 activity, were transiently increased in passage 9, meaning that they correlated with the expression of APE1/Ref-1. Overexpression of APE1/Ref-1 suppressed superoxide production and decreased SA b-gal in hBMSCs. In conclusion, intracellular superoxide accumulation appears to be the main cause of the senescence of hBMSCs, and overexpression of APE1/Ref-1 can rescue cells from the senescence phenotype. Maintaining characteristics of hBMSCs by regulating intracellular reactive oxygen species production can contribute to tissue regeneration and to improved cell therapy.

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“…Selenium, as sodium selenite, has been reported as a co-factor for glutathione peroxidase and other proteins and used as an anti-oxidant in medium (Wu et al 1973). In cell culture system, sodium selenite protected cell from oxidative damage, free radicals and obstructed lipid perioxide products (Ebert et al 2006). Transferrin and Selenium played a role in the antioxidant defense system in the oocyte, which was essential for the catalytic activity of glutathione peroxidase (Cerri et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Effect of Fibroblast Growth Factor 2 (FGF2) and Insulin Transferrin Selenium (ITS) on In Vitro Maturation, Fertilization and Embryo Development in Sheep

Mondal

Mor

Reddy

et al. 2015

Braz. arch. biol. technol.

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The present study evaluated the effect of fibroblast growth factor 2 (FGF2) and insulin-transferrin-selenium (ITS) to the in vitro maturation and embryo culture media on ovine oocyte maturation, cleavage and embryo development. Oocytes having more than five layers of unexpanded cumulus cells and granular homogenous ooplasm were cultured into 50 μL droplets of eight different culture systems: (i) TCM-199 (Tissue Culture Medium-199); (ii) TCM-199+10 ng/mL FGF2; (iii) TCM-199+20 ng/mL FGF2; (iv) TCM-199+30 ng/mL FGF2; (v) TCM-199+10 ng/mL ITS; (vi) TCM-199+20 ng/mL ITS; (vii) TCM-199+30 ng/mL ITS and (viii) TCM-199+20 ng/mL ITS+20 ng

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Selenium Supplementation Restores the Antioxidative Capacity and Prevents Cell Damage in Bone Marrow Stromal Cells In Vitro

Abstract: STEM CELLS 2006;24: 1226 -1235

Cited by 174 publications

References 72 publications

Lithium as a prooxidant? A possible protective role of selenium – in vitro study

Lithium as a prooxidant? A possible protective role of selenium – in vitro study

Downregulation of APE1/Ref-1 Is Involved in the Senescence of Mesenchymal Stem Cells

Effect of Fibroblast Growth Factor 2 (FGF2) and Insulin Transferrin Selenium (ITS) on In Vitro Maturation, Fertilization and Embryo Development in Sheep

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