Self-assembly of gold supraparticles with crystallographically aligned and strongly coupled nanoparticle building blocks for SERS and photothermal therapy

Paterson, Sureyya; Thompson, Sebastián; Gracie, J. Alastair; Wark, Alastair W.; Rica, Roberto de la

doi:10.1039/c6sc02465c

Cited by 15 publications

(14 citation statements)

References 66 publications

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“…Surface Enhanced Raman Scattering (SERS) could then be performed due to the close contact of the tightly packed Au nanoparticles with the Fmoc-dipeptides, associated with the formation of "hot-spots". 53,54 The SERS spectrum of the Fmoc-CF is shown in Figure 6B, demonstrating the dramatic enhancement of the Raman signal over the bare Fmoc-CF supramolecular structures. The strong bands at 2845 and 2880 cm -1 could be assigned to the symmetric stretch of the CH 2 (CO) group and the C-H stretching mode, respectively.…”

Section: Figurementioning

confidence: 99%

Unravelling the 2D self-assembly of Fmoc-dipeptides at fluid interfaces

et al. 2018

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Section: Figurementioning

confidence: 99%

Unravelling the 2D self-assembly of Fmoc-dipeptides at fluid interfaces

et al. 2018

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“…The method consists of attaching the enzyme to photothermal nanoheaters, for example, to gold nanorods. The nanoheaters generate plasmonic heat when irradiated with a laser resonant with their localized surface plasmon resonance . The subsequent increase in temperature is the highest in the vicinity of the nanorods, where the enzymes are located.…”

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confidence: 99%

“…Any damage to the cell membrane resulting from photothermal effects in these experiments was evaluated by adding ethidium bromide to the culture plates. This compound only becomes fluorescent upon interacting with the nuclei of cells whose membrane has been damaged . In Figure a–d when CHO cells were irradiated for 15, 30, 60, or 90 s no damage to the cell membrane was registered.…”

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confidence: 99%

Light‐Triggered Inactivation of Enzymes with Photothermal Nanoheaters

Thompson

Paterson

Azab

et al. 2017

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This version is available at https://strathprints.strath.ac.uk/59696/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge.Any correspondence concerning this service should be sent to the Strathprints administrator: strathprints@strath.ac.ukThe Strathprints institutional repository (https://strathprints.strath.ac.uk) is a digital archive of University of Strathclyde research outputs. It has been developed to disseminate open access research outputs, expose data about those outputs, and enable the management and persistent access to Strathclyde's intellectual output. conditions. The results shown here pave the way for using the proposed methodology to discern the role of target enzymes in intracellular signaling pathways. 3Enzymes are biological catalysts involved in crucial cellular processes such as signaling pathways, DNA replication, and protein expression. In biotechnology, enzymes are used for manufacturing biosensors, [1] medicines, [2] fuels [3] and foodstuffs. [4] A methodology for inactivating a target enzyme on demand could be useful for discerning the role of the biocatalyst in a particular cell function. [5] A light-triggered inactivation mechanism would be ideal for these applications since it would enable turning off the enzyme activity noninvasively and on demand. Current methods for controlling the activity of enzymes with light require the synthesis of photoresponsive enzyme substrates, i.e. a molecule that changes its conformation upon irradiation at a particular wavelength. [6] While this approach allows a fine control over the activity of a given enzyme, it can only be applied to those enzymes whose substrate can be synthesized in a laboratory. Furthermore, enzymes show exquisite specificity for the conversion of a particular substrate. This means that different lightresponsive molecules must be synthesized for each specific enzyme, which is very timeconsuming. Moreover, supplying synthetic substrates may be cumbersome in intracellular or in vivo studies, in which the photoresponsive molecules may need to permeate cell membranes and compete with naturally occurring substrates in order to inactivate the enzymes. In this context it would be highly desirable to find a generic methodology that allowed inactivating any target enzyme remotely regardless of its substrate specificity and in different biological scenarios, such as in the presence of other enzym...

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“… 1 , 2 The resultant intense fields in hotspots make it possible to realize single molecule ultrasensitive detection via surface enhanced Raman scattering (SERS). In recent years, it was elucidated that the enormous field enhancement could also lead to the efficient generation of hot charge carriers, 3 a including energetic electrons and holes, which are powerful for driving chemical reactions and the conversion of optical energy into thermal or chemical energy. 3 b Thus, besides molecular sensing, the possibility to extract and use hot carriers generated offers tremendous opportunities and triggers new research in a broad variety of exciting applications such as photocatalysis and solar-to-chemical or electrical energy conversion.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, it was elucidated that the enormous field enhancement could also lead to the efficient generation of hot charge carriers, 3 a including energetic electrons and holes, which are powerful for driving chemical reactions and the conversion of optical energy into thermal or chemical energy. 3 b Thus, besides molecular sensing, the possibility to extract and use hot carriers generated offers tremendous opportunities and triggers new research in a broad variety of exciting applications such as photocatalysis and solar-to-chemical or electrical energy conversion. 4 Apparently, for better and efficient implementation of these plasmon-assisted applications, creating hotspots with high field enhancements, especially those with an integrated capability of selectively trapping targeted molecules into hotspots, is of critical importance.…”

Section: Introductionmentioning

confidence: 99%