Semi-automated vs. visual reading of urinalysis dipsticks.

Peele, James; Gadsden, Richard H.; Crews, R

doi:10.1093/clinchem/23.12.2242

Cited by 50 publications

(33 citation statements)

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“… 12 18–20 Two studies have shown that automatic reading of urine test strips is more accurate than visual reading of the same test strip. 6 7 Peele et al already reported on this in 1977 using a semiautomated dipstick reader in a laboratory setting. However, technology has developed since and the described analyser is no longer available.…”

Section: Discussionmentioning

confidence: 99%

“…However, technology has developed since and the described analyser is no longer available. 6 Tighe 7 performed his study in a laboratory setting, using aqueous spiked samples instead of patient samples, and although they did evaluate a POCT urine analyser, it was not compared with a laboratory reference standard.…”

Section: Discussionmentioning

confidence: 99%

“…4 5 It has been suggested that the use of point of care testing (POCT) urine analysers will increase the quality of urinalysis. 6 7 With the introduction of commercially available POCT urine analysers, it may be possible to improve the quality of urinalysis in general practice. These analysers, therefore, need to have good analytical performance and agreement with laboratory standards, but data are lacking for the different parameters.…”

Section: Introductionmentioning

confidence: 99%

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Analytical performance, agreement and user-friendliness of six point-of-care testing urine analysers for urinary tract infection in general practice

Schot

Delft²,

Kooijman-Buiting³

et al. 2015

BMJ Open

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ObjectiveVarious point-of-care testing (POCT) urine analysers are commercially available for routine urine analysis in general practice. The present study compares analytical performance, agreement and user-friendliness of six different POCT urine analysers for diagnosing urinary tract infection in general practice.SettingAll testing procedures were performed at a diagnostic centre for primary care in the Netherlands. Urine samples were collected at four general practices.Primary and secondary outcome measuresAnalytical performance and agreement of the POCT analysers regarding nitrite, leucocytes and erythrocytes, with the laboratory reference standard, was the primary outcome measure, and analysed by calculating sensitivity, specificity, positive and negative predictive value, and Cohen's κ coefficient for agreement. Secondary outcome measures were the user-friendliness of the POCT analysers, in addition to other characteristics of the analysers.ResultsThe following six POCT analysers were evaluated: Uryxxon Relax (Macherey Nagel), Urisys 1100 (Roche), Clinitek Status (Siemens), Aution 11 (Menarini), Aution Micro (Menarini) and Urilyzer (Analyticon). Analytical performance was good for all analysers. Compared with laboratory reference standards, overall agreement was good, but differed per parameter and per analyser. Concerning the nitrite test, the most important test for clinical practice, all but one showed perfect agreement with the laboratory standard. For leucocytes and erythrocytes specificity was high, but sensitivity was considerably lower. Agreement for leucocytes varied between good to very good, and for the erythrocyte test between fair and good. First-time users indicated that the analysers were easy to use. They expected higher productivity and accuracy when using these analysers in daily practice.ConclusionsThe overall performance and user-friendliness of all six commercially available POCT urine analysers was sufficient to justify routine use in suspected urinary tract infections in general practice.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analytical performance, agreement and user-friendliness of six point-of-care testing urine analysers for urinary tract infection in general practice

Schot

Delft²,

Kooijman-Buiting³

et al. 2015

BMJ Open

View full text Add to dashboard Cite

show abstract

“…These tests allow for the diagnosis of a variety of conditions and diseases including kidney disease, urinary tract infections, carbohydrate metabolism disorders, and liver disease [4,15,16]. For more sophisticated urinalysis tests, the dipsticks can be analyzed by a detector that analyzes the color of the test pads in order to improve the accuracy and precision of the tests [3,17]. Another common application for dipstick tests is for the analysis of water samples.…”

Section: Dipstick Devicesmentioning

confidence: 99%

Paper-based diagnostic devices

Schultz

Noxon

Sisley

et al. 2017

Portable Biosensors and Point-of-Care Systems

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“…Pen-based devices take advantage of flexible patterning, inexpensive costs, and small volumes, thereby saving time and increasing efficiency. 1 2 3 Pen-based techniques have been used to pattern surfaces for cell studies. 4 5 6 Such approaches can also be used to apply patterns on nano- or micro-scales for biological uses.…”

Section: Introductionmentioning

confidence: 99%

Marker Pen Device with Concentration Gradient Nib for Antibiotic Susceptibility Testing

et al. 2018

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BackgroundPen-based devices have emerged as useful tools for measuring pH and glucose, and for fabricating microchannels and microarrays. Pen-based devices take advantage of flexible patterning, inexpensive costs, and small volumes, thereby saving time and increasing efficiency. We have developed a gradient nib marker pen device that generated simultaneously different antibiotic concentrations in bacteria antibiotic susceptibility testing (AST).MethodsThe device can deposit on the target surface with the antibiotic gradient. The designed polyester fiber nibs are a highly uniform porosity with unidirectional orientation and produce a visible gradient pattern.ResultsWe have demonstrated and quantitatively analyzed bacterial growth after antibiotic marking. The antibiotic marking produces an inhibition zone of bacterial growth. The inhibition zones of bacterial growth are captured and converted to 8-bit grayscale images, and then quantified by gray values using the Image J program. A profile of the inhibition zone showed different gray values in response to bacterial viability.ConclusionThe gradient nib marker pen device can be used to determine the quantitative antibiotic concentration based on the relationship between gray values and bacterial density conveniently without requiring a series of dilution tubes, including nutrient medium, and diversely diluted antibiotics.

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Semi-automated vs. visual reading of urinalysis dipsticks.

Cited by 50 publications

References 0 publications

Analytical performance, agreement and user-friendliness of six point-of-care testing urine analysers for urinary tract infection in general practice

Analytical performance, agreement and user-friendliness of six point-of-care testing urine analysers for urinary tract infection in general practice

Paper-based diagnostic devices

Marker Pen Device with Concentration Gradient Nib for Antibiotic Susceptibility Testing

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