SEMI PURIFIKASI DAN KARAKTERISASI ENZIM PROTEASE Bacillus sp.

Naiola, Elidar; Widhyastuti, Nunuk

doi:10.23869/bphjbr.13.1.20078

Cited by 7 publications

(6 citation statements)

References 2 publications

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“…Aktivitas katalitik enzim bergantung pada integritas struktur sebagai protein. Enzim yang bekerja sebagai katalis dalam reaksi hidrolisis protein disebut enzim proteolitik atau enzim protease (Naiola, 2007;Prahasta, 2009). Bromelin merupakan salah satu enzim proteolitik yang mengkatalisasi penguraian protein menjadi asam amino melalui reaksi hidrolisis (Glider and Hargrove, 2002).…”

Section: Pendahuluanunclassified

Community Empowerment in Pineapple Agroindustry Waste Utilization as an Ingredient of Snacks in Subang Districts

Adi

Rifqi

Regitasari

et al. 2021

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Background:The development of agro-industry in Indonesia remains facing many obstacles. In addition to the environmental wasteproblem, the low ability to process products leads to their low added value, as faced bypineapple agro-industry associationin Barokah Agro Lestari (BAL). This community service activity is important to improve beneficial and economical value of pineapple agro-industry. Purpose: This activity aims to reduce the environmental burdenof pineapple agro-industry by product by implementing technology to give its added value as well as providing a healthy snack alternative for society. Method: This community service activity is carried out through counseling, training and education related to waste product of pineapple. These activities included the preparation phase, the selection of organic waste of pineapple, bioactive component testing, testing of nutrients and food formulation. Results:The community service program was conducted at PT Karya Masyarakat Mandiri in Subang District and attended by 30 pineapple farmers from Cirangkong village, gathered in Barokah Agro Lestari (BAL). Training was done by administering stages of activity systemically, exposure of the material using visual methods, cooking demonstration for the training activities of processing and product development. Conclusion: Community service activities and training in processing the pineapple stem waste can be a means of empowering local communities. Processing organic waste into food products help improving the community skills and motivation for business development through organic waste treatment.

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Section: Pendahuluanunclassified

Community Empowerment in Pineapple Agroindustry Waste Utilization as an Ingredient of Snacks in Subang Districts

Adi

Rifqi

Regitasari

et al. 2021

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“…Kandungan enzim bromelin lebih banyak terdapat pada bagian bongol buah yang telah matang daripada bagian lain dari tanaman nanas Herdyastuti (2006). Untuk memperoleh enzim bromelin dapat dilakukan proses isolasi dengan menggunakan cara memisahkan sel secara sentrifugasi kemudian dilakukan pemurnian dengan berbagai metode seperti gel filtrasi , pengendapan dan kromatografi Naiola and Widhyastuti (2007).…”

Section: Pendahuluanunclassified

Kinerja Fungsi Ginjal pada Kelinci (Lepus nigricollins) Diabetes yang diberi Ekstrak Bonggol Buah Nanas (Ananas comusus L.)

Rahmayanti

Ardiansyah

2019

Medicra

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Bromelain enzyme contained in pineapple (Ananas comosus L.) are enzyme that have a function as anti-inflamatory in diabetic treatment periodically, will be giving impact to kidney function performance. This study to determine the effect of pineapple hump extract (Ananas comosus L.) on creatinine level in diabetic rabbits. Rabbits were acclimated for 3 weeks than measured the creatinine level to know the first level. So, it induced by alloxan as much as 195 mg/kg during ±2 weeks for conditioning under hyperglicemia. Then measured the creatinine level from 6 groups, it given pineapple extract with different concentration, those are positive control (+), negative control (-), extract 25%, 50%, 75% and 100%. Then measured of creatinine level did in enzymatic photometric method. In group of concentration 25% the creatinine level from 0,58 mg/dl become 1,07 mg/dl, in concentration 50% from 1,55 mg/dl become 1,25 mg/dl, in concentration 75% from 1,63 mg/dl become 1,19 mg/dl and in concentration 100% from 1,58 mg/dl become 1,42 mg/dl. The result of the study were tested statiscally with one way Anova test, it showed p> 0,05. It show Pineapple hump extract has no effect on the role of diabetic rabbits kidney.

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“…Berdasarkan cara pemotongan ikatan peptida, enzim peptidase dapat dibagi menjadi eksopeptidase dan endopeptidase yang bekerja secara bersama-sama dalam memotong ikatan peptida pada suatu molekul protein (Mubarik et al, 2000). Eksopeptidase bekerja pada kedua ujung molekul protein, yang terdiri dari dua jenis enzim yaitu karboksipeptidase dan amino peptidase (Naiola et al, 2007).…”

Section: Hasil Dan Pembahasanunclassified

Karakterisasi Sifat Biokimia Isolat Bakteri Kitinolitik Asal Tambak Udang

Wang¹,

Kirami²,

Khaeruni³

2014

J-BEKH

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Informasi tentang sifat-sifat biokimia yang dimiliki oleh suatu isolat bakteri memberi peluang dalam mengungkap potensi isolat untuk pemanfaatan pada berbagai bidang. Penelitian ini bertujuan untuk mengetahui sifat-sifat biokimia dari bakteri kitinolitik isolat SSA2.B4.1 dan SSD2A7.1 yang berasal dari tambak udang di Kabupaten Pinrang dan Maros, Sulawesi Selatan. Penelitian dilakukan dengan uji-uji biokimia terhadap kedua isolat tersebut. Hasil penelitian menunjukkan isolat bakteri SSA2B4.1 berjenis gram positif dan mampu menggunakan berbagai senyawa karbohidrat sebagai sumber karbon, sedangkan SSD2A7.1 berjenis gram negatif dan tidak mampu menggunakan berbagai senyawa karbohidrat sebagai sumber karbon. Isolat bakteri SSA2.B4.1 selain mampu mensekresi enzim kitinase, juga mampu mensekresi enzim selulase dan protease, sehingga berpotensi untuk diaplikasikan pada bidang industri deterjen, proses deproteinasi dalam pembuatan kitin serta sebagai agen hayati patogen tanaman.

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SEMI PURIFIKASI DAN KARAKTERISASI ENZIM PROTEASE Bacillus sp.

Cited by 7 publications

References 2 publications

Community Empowerment in Pineapple Agroindustry Waste Utilization as an Ingredient of Snacks in Subang Districts

Community Empowerment in Pineapple Agroindustry Waste Utilization as an Ingredient of Snacks in Subang Districts

Kinerja Fungsi Ginjal pada Kelinci (Lepus nigricollins) Diabetes yang diberi Ekstrak Bonggol Buah Nanas (Ananas comusus L.)

Karakterisasi Sifat Biokimia Isolat Bakteri Kitinolitik Asal Tambak Udang

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