Semicontinuous silver films for protein sensing with SERS

Drachev, Vladimir P.; Thoreson, Mark D.; Khaliullin, Eldar N.; Sarychev, Andrey K.; Zhang, Dongmao; Ben‐Amotz, Dor; Shalaev, Vladimir M.

doi:10.1117/12.510572

Cited by 12 publications

(13 citation statements)

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“…The adaptive feature of our samples is exciting because it allows the study of differently-sized analytes, from the very small organic molecules to larger proteins or viruses and beyond, using the same types of substrates [30]. Adaptive metal films made with silver, herein called adaptive silver films (ASFs), have been successfully used in insulin isomer SERS studies, antibody-antigen binding experiments, protein phosphorylation studies and microarray experiments [44,43,30,45,46]. The interaction of biological molecules with a typical SERS substrate often leads to structural and functional changes in the molecules, and hence changes in the detected signal as compared to the normal (unenhanced) Raman signal.…”

Section: Adaptive Sers-active Substratesmentioning

confidence: 99%

Studies on metal-dielectric plasmonic structures.

Chettiar

Liu

Thoreson

et al. 2010

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The interaction of light with nanostructured metal leads to a number of fascinating phenomena, including plasmon oscillations that can be harnessed for a variety of cutting-edge applications. Plasmon oscillation modes are the collective oscillation of free electrons in metals under incident light. Previously, surface plasmon modes have been used for communication, sensing, nonlinear optics and novel physics studies. In this report, we describe the scientific research completed on metal-dielectric plasmonic films accomplished during a multi-year Purdue Excellence in Science and Engineering Graduate Fellowship sponsored by Sandia National Laboratories. A variety of plasmonic structures, from random 2D metal-dielectric films to 3D composite metal-dielectric films, have been studied in this research for applications such as surface-enhanced Raman sensing, tunable superlenses with resolutions beyond the diffraction limit, enhanced molecular absorption, infrared obscurants, and other real-world applications.

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Section: Adaptive Sers-active Substratesmentioning

confidence: 99%

Studies on metal-dielectric plasmonic structures.

Chettiar

Liu

Thoreson

et al. 2010

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“…Various metal films and fabrication techniques are used for this purpose: physical vacuum deposition (PVD) [20,21], electrochemical deposition from the solution [22][23][24], deposition from a colloidal solution of nanoparticles [25][26][27][28], electron-beam lithography [29,30], plasma-chemical lithography [31]. All the recently used metal thin films and surfaces for SERS-based sensorics usually based on already known general principles of creating surfaces for the successful registration of the giant Raman spectra [32].…”

Section: Introductionmentioning

confidence: 99%

SERS-Active Substrates Nanoengineering Based on e-Beam Evaporated Self-Assembled Silver Films

et al. 2019

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Surface-enhanced Raman spectroscopy (SERS) has been intensely studied as a possible solution in the fields of analytical chemistry and biosensorics for decades. Substantial research has been devoted to engineering signal enhanced SERS-active substrates based on semi-continuous nanostructured silver and gold films, or agglomerates of micro- and nanoparticles in solution. Herein, we demonstrate the high-amplitude spectra of myoglobin precipitated out of ultra-low concentration solutions (below 10 μg/mL) using e-beam evaporated continuous self-assembled silver films. We observe up to 105 times Raman signal amplification with purposefully designed SERS-active substrates in comparison with the control samples. SERS-active substrates are obtained by electron beam evaporation of silver thin films with well controlled nanostructured surface morphology. The characteristic dimensions of the morphology elements vary in the range from several to tens of nanometers. Using optical confocal microscopy we demonstrate that proteins form a conformation on the surface of the self-assembled silver film, which results in an effective enhancement of giant Raman scattering signal. We investigate the various SERS substrates surface morphologies by means of atomic force microscopy (AFM) in combination with deep data analysis with Gwyddion software and a number of machine learning techniques. Based on these results, we identify the most significant film surface morphology patterns and evaporation recipe parameters to obtain the highest amplitude SERS spectra. Moreover, we demonstrate the possibility of automated selection of suitable morphological parameters to obtain the high-amplitude spectra. The developed AFM data auto-analysis procedures are used for smart optimization of SERS-active substrates nanoengineering processes.

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“…On the other hand, from a practical point of view, random nanostructures, such as aggregates of a large number of colloidal nanoparticles or high-density arrays of granular nanoparticles with irregular shapes, are also useful for SERS. [11][12][13][14] The properties of these random nanostructures are typically discussed qualitatively based on an analogy with existing theoretical results for simple nanostructures with regular shapes. The influence of irregularities in sizes, shapes, and arrangement on the local field enhancement has not been well understood.…”

Section: Introductionmentioning

confidence: 99%

Near-field simulation of obliquely deposited surface-enhanced Raman scattering substrates

Jen¹,

Suzuki²,

Wang³

et al. 2012

Journal of Applied Physics

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A semicontinuous gold layer on a template with a zigzag structure has been demonstrated to be a highly sensitive substrate for surface-enhanced Raman scattering (SERS). Obliquely deposited gold layers on different templates yield different SERS strengths. In this work, a finite-difference time-domain method is utilized to analyze the electric field distribution within the gold layer when the film is illuminated by light. The random gold nanostructures are scanned and transformed into binary images to allow them to be simulated. The field intensities and SERS enhancement factors of different samples are calculated and compared. Our analysis results reveal the near-field characteristics of strong SERS and agree closely with experimental measurements.

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Semicontinuous silver films for protein sensing with SERS

Cited by 12 publications

References 0 publications

Studies on metal-dielectric plasmonic structures.

Studies on metal-dielectric plasmonic structures.

SERS-Active Substrates Nanoengineering Based on e-Beam Evaporated Self-Assembled Silver Films

Near-field simulation of obliquely deposited surface-enhanced Raman scattering substrates

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