Seminal plasma reactive oxygen species-antioxidants relationship with varicocele grade

Abstract

This work aimed to assess seminal plasma reactive oxygen species (ROS)-antioxidants relationship with varicocele grade in infertile men with oligoasthenoteratozoospermia (OAT). The study included 89 infertile OAT men with varicocele divided into grade I (n = 22) and grade II (n = 43), grade III (n = 24) and compared with 20 healthy fertile controls. In their seminal plasma, two ROS parameters (malondialdehyde [MDA], hydrogen peroxide [H(2) O(2) ]) and four antioxidants (superoxide dismutase [SOD], catalase [Cat], glutathione peroxidase [GPx], vit.C) were estimated. There was significant increase in seminal MDA, H(2) O(2) and significant decrease in seminal SOD, Cat, GPx, vit.C in varicocele-associated OAT cases when compared with the controls. Compared with grade I cases, varicocele cases with grades II, III demonstrated significant increase in estimated seminal MDA, H(2) O(2) and significant decrease in seminal SOD, Cat, GPx, vit.C. It is concluded that seminal oxidative stress (OS) is related to increased varicocele grade in infertile OAT men associated with varicocele.

Increased Oxidative DNA Damage in Seminal Plasma of Infertile Men with Varicocele

“…Increased Reactive Oxidative Species (ROS) levels in seminal plasma are strongly correlated with varicocele degree and are reported to decrease after varicocelectomy [20,21]. The present study also documented that varicocele degree are positively correlated with seminal plasma 8-OHdG level and sperm DFI in the varicocele group.…”

Association of Seminal Plasma Total Antioxidant Capacity and Malondialdehyde Levels With Sperm Parameters in Infertile Men With Varicocele

“…In addition, several studies have shown higher MDA levels as lipid peroxidation markers in infertile men. Mostafa et al found higher levels of MDA in the internal spermatic venous blood compared to those in the peripheral fluid (32). Similarly, in a study by Altunoluk et al, MDA levels were greater in the varicocele group (33).…”

Seminal Clusterin Gene Expression Associated with Seminal Variables in Fertile and Infertile Men

“…ROS has been found in the seminiferous tubules and seminal plasma of most infertile patients, especially those with OAT. 20 The concentration of malondialdehyde, a marker of lipid peroxidation, was almost twice as high in the sperm pellet suspensions of men with asthenozoospermia and oligoasthenozoospermia as those with normozoospermia. 21 Previously, O'Bryan et al described CLU as present only in human abnormal spermatozoa, 14 in accord with the consensus that in defective semen samples high ROS generation is associated with decreased seminal antioxidants.…”