Sensing oxygen at the millisecond time-scale using an ultra-microelectrode array (UMEA)

Rossem, Fleur van; Bomer, Johan G.; Boer, Hans L. de; Abbas, Yawar; Weerd, Eddy de; Berg, Albert van den; Gac, Séverine Le

doi:10.1016/j.snb.2016.06.156

Cited by 16 publications

(17 citation statements)

References 38 publications

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“…Hydrogen peroxide produced is directly proportional to the concentration of oxygen present in the sample. The generated peroxide is subsequently oxidised at WE Pt array, seen in equation (2). The electrons gained by the WE Pt in this reaction are measured and used as an indication of the oxygen level present in the sample.…”

Section: Electrochemical Oxygen Sensormentioning

confidence: 99%

“…Electrochemical sensors are gaining traction for a variety of new applications including point of care clinical diagnostics and in the field environmental monitoring. To address this growing need, nano and ultra-micro scale electrochemical sensors are currently being developed by several research groups worldwide for point of sample tests [1][2][3][4]. Although many new electrochemical sensing technologies have and are being developed, it is difficult to deploy them outside of a research laboratory due to the expensive bulky benchtop equipment required to interrogate these electrochemical sensors.…”

Section: Introductionmentioning

confidence: 99%

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Portable Data Acquisition System for Nano and Ultra-Micro Scale Electrochemical Sensors

Murphy¹,

Seymour²,

Rohan³

et al. 2020

IEEE Sensors J.

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This work describes a flexible and portable data acquisition system that has been developed to interface to nano and ultra-micro scale electrochemical sensors at the point of use. It can perform a range of voltammetric tests, including Cyclic Voltammetry, Square Wave Voltammetry and Generator Collector Voltammetery. The data acquisition system interfaces to a smartphone, operates from a rechargeable battery and is of suitable form factor to ensure that it's fully portable. By utilising commercially available components, this system has been developed to lower the barrier for entry for the development of emerging portable electrochemical sensing technologies at micro and nano scale. To show the full range of functionality of the system, a use case involving river water quality monitoring is presented through generation of a calibration curve, using a recently developed Tyndall National Institute ultra-microband electrochemical sensor, for the detection of dissolved oxygen in river water.

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Section: Electrochemical Oxygen Sensormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Portable Data Acquisition System for Nano and Ultra-Micro Scale Electrochemical Sensors

Murphy¹,

Seymour²,

Rohan³

et al. 2020

IEEE Sensors J.

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show abstract

“…The sterilization, coating (collagen-I, Corning) and seeding protocol for the chip were similar as previously described for the Caco-2 cells. The hCMEC/D3 cells (passage number [31][32][33][34][35] were seeded at a seeding density 2 × 10 5 cells per cm 2 in the bottom (endothelial) channels of the collagencoated chips. The chip was then immediately inverted so the cells could attach on another side of the membrane and left in the incubator (37°C, 5% CO 2 ) for 1 hour.…”

Section: Cell Culturementioning

confidence: 99%

“…pH, nitric oxide or oxygen) and simultaneously still facilitate impedance spectroscopy. [33][34][35][36][37]…”

Section: Lab On a Chip Papermentioning

confidence: 99%

Measuring barrier function in organ-on-chips with cleanroom-free integration of multiplexable electrodes

et al. 2021

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“…However, the presence of the membrane increases the response time and complicates the sensor fabrication process. The ultra-microelectrode array (UMEA) is another method that is used for the construction of an amperometric DO sensor that is insensitive to the stirring of the analytes [3][4][5][6][7]. This is because the detection process is no longer limited by oxygen transport due to a shorter diffusion length.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of a Nitrogen and Boron-Doped Reduced Graphene Oxide Membrane-Less Amperometric Sensor for Measurement of Dissolved Oxygen in a Microbial Fermentation

2020

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Dissolved oxygen (DO) is an important parameter to monitor in processes applicable in, for example, water technology and fermentation. In this study, we report the manufacturing of a membrane-less amperometric sensor that is based on the electrocatalytic reduction of oxygen. The sensor was tested in pH-neutral KNO3 solutions and in a microbial fermentation to monitor the consumption of dissolved oxygen. The nitrogen and boron-doped reduced graphene oxide (N,B-HRGO) is used as an electrocatalyst for oxygen reduction. One step co-doping of nitrogen and boron on graphene oxide is performed using the hydrothermal method. The sensor responded linearly to the DO concentration. A sensitivity of 0.2 µA/mg·L−1 O2 is obtained for the DO concentration of 1.5 to 10 mg·L−1 O2. The membrane-less N,B-HRGO based DO sensor is successfully tested in an Amycolotopsis methanolica fermentation by monitoring the dissolved oxygen in real-time. The sensor detected the consumption of oxygen during the growth of A. methanolica, which shows the compatibility of N,B-HRGO as electrode material for amperometric measurement of dissolved oxygen in aerobic fermentation. This membrane-less amperometric sensor can be used to fabricate microdevices for microbioreactor applications.

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Sensing oxygen at the millisecond time-scale using an ultra-microelectrode array (UMEA)

Cited by 16 publications

References 38 publications

Portable Data Acquisition System for Nano and Ultra-Micro Scale Electrochemical Sensors

Portable Data Acquisition System for Nano and Ultra-Micro Scale Electrochemical Sensors

Measuring barrier function in organ-on-chips with cleanroom-free integration of multiplexable electrodes

Fabrication of a Nitrogen and Boron-Doped Reduced Graphene Oxide Membrane-Less Amperometric Sensor for Measurement of Dissolved Oxygen in a Microbial Fermentation

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