Sensitive and Convenient Detection of microRNAs Based on Cascade Amplification by Catalytic DNAzymes

Tian, Tian; Xiao, Heng; Zhang, Zhengan; Long, Yuelin; Peng, Shuang; Wang, Shaoru; Zhou, Xiang; Liu, Song-Mei; Zhou, Xin

doi:10.1002/chem.201203344

Cited by 82 publications

(42 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hemin as an active cofactor for many enzymes can catalyze the peroxidation reaction. When incubation with guanine-rich DNA aptamer, hemin can intercalate into a G-quadruplex structure to form a hemin/G-quadruplex DNAzyme with peroxidase-like ability [34,35,36]. Ai and co-workers reported a signal-amplified electrochemical miRNAs biosensor by employing hemin-G-quadruplex as the signal unit [37].…”

Section: Nanomaterials-based Electrochemical Strategies For Mirnasmentioning

confidence: 99%

Nanomaterials-Based Sensing Strategies for Electrochemical Detection of MicroRNAs

Xia

Zhang

2014

Materials

View full text Add to dashboard Cite

MicroRNAs (miRNAs) play important functions in post-transcriptional regulation of gene expression. They have been regarded as reliable molecular biomarkers for many diseases including cancer. However, the content of miRNAs in cells can be low down to a few molecules per cell. Thus, highly sensitive analytical methods for miRNAs detection are desired. Recently, electrochemical biosensors have held great promise as devices suitable for point-of-care diagnostics and multiplexed platforms for fast, simple and low-cost nucleic acid analysis. Signal amplification by nanomaterials is one of the most popular strategies for developing ultrasensitive assay methods. This review surveys the latest achievements in the use of nanomaterials to detect miRNAs with a focus on electrochemical techniques.

show abstract

Section: Nanomaterials-based Electrochemical Strategies For Mirnasmentioning

confidence: 99%

Nanomaterials-Based Sensing Strategies for Electrochemical Detection of MicroRNAs

Xia

Zhang

2014

Materials

View full text Add to dashboard Cite

show abstract

“…The proposed method is quite sensitive, 5 pM miRNA-141 could be easily detected ( Fig. S6 and S7) [23,24].…”

Section: Namementioning

confidence: 98%

Label-free fluorescence turn-on detection of microRNA based on duplex-specific nuclease and a perylene probe

Chen

et al. 2015

Analytica Chimica Acta

View full text Add to dashboard Cite

“…Others have used DNAzme/enzyme cascades as well [66,67]. Tang and co-workers used DNAzyme/enzyme cascade for an amplified detection of microRNAs [67].…”

Section: Dnazyme Cascade Amplificationmentioning

confidence: 99%

Nucleic acid detection using G-quadruplex amplification methodologies

Roembke

Nakayama

Sintim

2013

Methods

View full text Add to dashboard Cite

In the last decade, there has been an explosion in the use of G-quadruplex labels to detect various analytes, including DNA/RNA, proteins, metals and other metabolites. In this review, we focus on strategies for the detection of nucleic acids, using G-quadruplexes as detection labels or as enzyme labels that amplify detection signals. Methods to detect other analytes are briefly mentioned. We highlight various strategies, including split G-quadruplex, hemin-G-quadruplex conjugates, molecular beacon G-quadruplex or inhibited G-quadruplex probes. The tandem use of G-quadruplex labels with various DNA-modifying enzymes, such as polymerases (used for rolling circle amplification), exonucleases and endonucleases, is also discussed. Some of the detection modalities that are discussed in this review include fluorescence, colorimetric, chemiluminescence, and electrochemical methods.

show abstract

Sensitive and Convenient Detection of microRNAs Based on Cascade Amplification by Catalytic DNAzymes

Cited by 82 publications

References 58 publications

Nanomaterials-Based Sensing Strategies for Electrochemical Detection of MicroRNAs

Nanomaterials-Based Sensing Strategies for Electrochemical Detection of MicroRNAs

Label-free fluorescence turn-on detection of microRNA based on duplex-specific nuclease and a perylene probe

Nucleic acid detection using G-quadruplex amplification methodologies

Contact Info

Product

Resources

About