Sensitive Detection of Caffeic Acid and Rutin via the Enhanced Anodic Electrochemiluminescence Signal of Luminol

Abstract

The electrooxidation of phenolic groups of caffeic acid and rutin promote anodic electrochemiluminescence (ECL) luminol substantially. A sensitive, and cost-effective ECL method has thus been developed to detect caffeic acid, ranging from 0.1 to 5.0 μM, with a detection limit of 0.1 μM and rutin ranging from 0.2 to 25 μM with a detection limit of 0.12 μM. Contrarily, phenolic compounds quench the weak cathodic ECL of luminol. Both of anodic and cathodic ECL mechanisms of luminol in the presence of phenolic compounds are analyzed. The method based on the boomed anodic ECL of luminol is comparable to those based on Ru(bpy)3 2+ and S2O8 2-/O2 systems. A lower onset potential and price than the other ECL reagents would realize its widely applications in the detection of phenolic compounds in food and medicine.

Deciphering electrochemiluminescence generation from luminol and hydrogen peroxide by imaging light emitting layer

“…For example, different mechanisms have been proposed for various electrode materials [18] , including gold [ 19 , 20 ], platinum [21] , indium tin oxide glass [22] , and carbon-based electrodes [ 14 , [23] , [24] , [25] ]. Moreover, a multiplicity of mechanisms exist in terms of experimental conditions, such as the applied potential [19] , and solution pH [26] . Current methodologies used for studying ECL mechanisms of luminol are usually based on the conventional cyclic voltammetry and ECL intensity-potential measurements [27] , [28] , [29] , [30] , [31] , [32] .…”