Sensitive identification of milk protein allergens using on-line combination of transient isotachophoresis/micellar electrokinetic chromatography and capillary isoelectric focusing in fused silica capillary with roughened part

Capillary electromigration methods for food analysis and Foodomics: Advances and applications in the period March 2021 to March 2023

“…The results obtained showed a great variation in the protein content of heat-treated milk samples and a low variation in the metal content in samples of different brands and prices. In an interesting work, Horká et al developed a CE-UV approach for the sensitive identification of milk protein allergens [36]. This approach was based on the on-line combination of transient isotachophoresis/micellar electrokinetic chromatography (tITP/MEKC) for the separation of the four important whey proteins (bovine serum albumin [BSA], α-lactalbumin [α-LA], and β-lactoglobulin A and β-actoglobulin B) in large volumes of samples.…”

Advances in separation and identification of biologically important milk proteins and peptides

“…Another method has been used for the separation of whey proteins BSA, α-LA, and two isoforms β-LG A and β-LG B, in which electrophoresis and LC were combined [99]. Here, novel features were used, that is, (i) online connection of transient isotachophoresis/micellar electrokinetic chromatography with capillary IEF, (ii) fused silica capillary (100 µm id and 360 µm od; 500 mm length), in which a part of its inner surface was roughened using supercritical water, and (iii) dynamic adsorption of milk proteins into layer of PEG 4000 polymer formed on the capillary roughened surface.…”

Modifying last layer in polyelectrolyte multilayer coatings for capillary electrophoresis of proteins

“…Different factors can explain this reduction, among which radial gradient temperature inside the capillary [5] due to Joule effect and capillary cooling, electromigration dispersion [6,7], capillary coiling [8], or protein adsorption onto the capillary wall [3,[9][10][11][12][13]. Monolayer coatings and successive multiple ionic-polymer layer (SMIL) capillary coatings were developed to limit protein adsorption [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28]. Pioneered in the late 1960 [29], multilayered polyelectrolyte coatings were rediscovered at the beginning of the 90s by Decher et al [30,31] and firstly applied to CE in 1998 [32].…”