Sensitivity for the Detection of a Clonally Rearranged Antigen Receptor Gene in Endoscopically Obtained Biopsy Specimens from Canine Alimentary Lymphoma

Fukushima, Kenjiro; Ohno, Koichi; Koshino-Goto, Yuko; Uchida, Kazuyuki; Nomura, Kohji; Takahashi, Masashi; Nakashima, Ko; Fujino, Yasuhito; Tsujimoto, Hajime

doi:10.1292/jvms.001673

Cited by 34 publications

(24 citation statements)

References 12 publications

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“…The sensitivity of PARR for detecting clonal cell proliferations was lower than what has been published for other types of canine lymphoma 8 but higher than previously reported by others (66.7%) for canine intestinal lymphoma. 22 The increase in sensitivity in our study may be due to the use of capillary electrophoresis rather than a polyacrylamide gel, leading to an increased resolution and thereby sensitivity. We performed PARR testing in duplicate to ensure that no single bands representing pseudoclones were misinterpreted as a clonal cell population.…”

Section: Discussionmentioning

confidence: 84%

Distinguishing Intestinal Lymphoma From Inflammatory Bowel Disease in Canine Duodenal Endoscopic Biopsy Samples

et al. 2014

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Inflammatory bowel disease (IBD) and intestinal lymphoma are intestinal disorders in dogs, both causing similar chronic digestive signs, although with a different prognosis and different treatment requirements. Differentiation between these 2 conditions is based on histopathologic evaluation of intestinal biopsies. However, an accurate diagnosis is often difficult based on histology alone, especially when only endoscopic biopsies are available to differentiate IBD from enteropathy-associated T-cell lymphoma (EATL) type 2, a small cell lymphoma. The purpose of this study was to evaluate the utility of histopathology; immunohistochemistry (IHC) for CD3, CD20, and Ki-67; and polymerase chain reaction (PCR) for antigen receptor rearrangement (T-cell clonality) in the differential diagnosis of severe IBD vs intestinal lymphoma. Endoscopic biopsies from 32 dogs with severe IBD or intestinal lymphoma were evaluated. The original diagnosis was based on microscopic examination of hematoxylin and eosin (HE)-stained sections alone followed by a second evaluation using morphology in association with IHC for CD3 and CD20 and a third evaluation using PCR for clonality. Our results show that, in contrast to feline intestinal lymphomas, 6 of 8 canine small intestinal lymphomas were EATL type 1 (large cell) lymphomas. EATL type 2 was uncommon. Regardless, in dogs, intraepithelial lymphocytes were not an important diagnostic feature to differentiate IBD from EATL as confirmed by PCR. EATL type 1 had a significantly higher Ki-67 index than did EATL type 2 or IBD cases. Based on the results of this study, a stepwise diagnostic approach using histology as the first step, followed by immunophenotyping and determining the Ki67 index and finally PCR for clonality, improves the accuracy of distinguishing intestinal lymphoma from IBD in dogs.

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Section: Discussionmentioning

confidence: 84%

Distinguishing Intestinal Lymphoma From Inflammatory Bowel Disease in Canine Duodenal Endoscopic Biopsy Samples

et al. 2014

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“…Based on careful histological review of morphology and immunophenotype results, the 2 polyclonal cases in this study could be interpreted as false-negative results. Given that severe infiltration of inflammatory lymphocytes concomitant with small cell intestinal lymphoma has been reported in cats with the same disease(17), this is the most likely cause of the false-negative result, leading to a decrease in the relative number of tumor cells present which may fall below the PCR detection limit(37,38). Other possible explanations for the 2 polyclonal results is unknown mutations in the variable (V) and joining (J) segments or lack of primer coverage of unknown V and J segments.…”

Section: Discussionmentioning

confidence: 99%

Clinical characteristics and outcome in dogs with small cell T‐cell intestinal lymphoma

Couto

Moore

Zwingenberger

et al. 2018

Vet Comparative Oncology

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Small cell intestinal lymphoma has not been well characterized in dogs. The objective of this study was to describe clinical characteristics and outcome in dogs with small cell intestinal lymphoma. We hypothesized that affected dogs would have prolonged survival compared to high-grade GI lymphoma. Pathology records were searched for dogs with histologically confirmed small cell GI lymphoma. Seventeen dogs with confirmed small cell intestinal lymphoma were identified, and clinical and outcome data were retrospectively collected. Histopathology was reviewed by a board-certified pathologist, and tissue sections were subjected to immunophenotyping and molecular clonality assessment. All dogs had small cell, T-cell, lymphoma confirmed within various regions of small intestine, with 1 dog also having disease in abdominal lymph nodes. All dogs had clinical signs attributable to GI disease; diarrhea (n=13) was most common. Ultrasonographic abnormalities were present in 8 of 13 dogs with abnormal wall layering (n=7) and hyperechoic mucosal striations (n=7) representing the most common findings. In total, 14 dogs received some form of treatment. The median survival time (MST) for all dogs was 279 days and the MST for the 14 dogs that received any treatment was 628 days. Dogs with anemia and weight loss at presentation had significantly shorter survival times and dogs that received a combination of steroids and an alkylating agent had significantly longer survival times. Small cell, T-cell, intestinal lymphoma is a distinct disease process in dogs, and those undergoing treatment may experience prolonged survival.

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“…PCR testing for clonal rearrangement of lymphocyte antigen was performed for 8 dogs in the Shiba group, and TCR gene clonal rearrangement was detected in 2 dogs. As the sensitivity and specificity of PCR testing are reportedly questionable to some extent for detecting alimentary lymphoma [9,17], there is some possibility that these were false-positive results. Unfortunately, necropsy could not be conducted on the 2 dogs, and we could not completely exclude the possibility of lymphoma.…”

Section: Discussionmentioning

confidence: 99%

A Retrospective Study in 21 Shiba Dogs with Chronic Enteropathy

Ohmi

Ohno

Uchida

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. We retrospectively studied the clinical and laboratory features and outcomes of chronic enteropathy in Shiba dogs. Among 99 dogs with chronic enteropathy, 21 Shiba dogs (21%) were included in the study (odds ratio, 7.14). No significant differences were seen in signalment, clinical signs, symptoms or laboratory profiles between the Shiba and non-Shiba groups. Severe histopathological lesions in the duodenum were a common finding in the Shiba group. The median overall duration of survival in the Shiba group was 74 days, while that of the dogs in the non-Shiba group could not be determined because more than half of the cases remained alive at the end of this study. The difference between the groups was statistically significant (P<0.0001). The 6-month and 1-year survival rates for the Shiba group were 46% and 31%, respectively. Conversely, the 6-month, 1-year and 3-year survival rates for the non-Shiba group were 83%, 74% and 67%. The results obtained here demonstrated that the Shiba dog is predisposed to chronic enteropathy and shows severe duodenum lesions and poor outcomes, indicating a breed-specific disease.KEY WORDS: breed-specific enteropathy, chronic enteropathy, Shiba.

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Sensitivity for the Detection of a Clonally Rearranged Antigen Receptor Gene in Endoscopically Obtained Biopsy Specimens from Canine Alimentary Lymphoma

Cited by 34 publications

References 12 publications

Distinguishing Intestinal Lymphoma From Inflammatory Bowel Disease in Canine Duodenal Endoscopic Biopsy Samples

Distinguishing Intestinal Lymphoma From Inflammatory Bowel Disease in Canine Duodenal Endoscopic Biopsy Samples

Clinical characteristics and outcome in dogs with small cell T‐cell intestinal lymphoma

A Retrospective Study in 21 Shiba Dogs with Chronic Enteropathy

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