Separation and characterization of proteins from green and etiolated shoots of rice (Oryza sativaL.): Towards a rice proteome

Komatsu, Setsuko; Afzal, Muhammad; Rakwal, Randeep

doi:10.1002/(sici)1522-2683(19990301)20:3<630::aid-elps630>3.0.co;2-z

Cited by 94 publications

(26 citation statements)

References 14 publications

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“…Rice seedlings were grown under white fluorescent light (6,000 lux, 12-h light period/day) at 25°C. Leaf sheaths, leaf blades, roots, and lamina joints were collected from 2-week-old rice seedlings (11)(12)(13)(14).…”

Section: Methodsmentioning

confidence: 99%

“…Concerning the rice plant, some well known studies have dealt with the construction of proteomes from complex origins, such as the leaf, embryo, endosperm, root, stem, shoot, and callus proteome (11)(12)(13)(14)(15). Proteomic studies to date have mainly focused on those changes in genome expression that are triggered by environmental factors.…”

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confidence: 99%

“…Proteomic studies to date have mainly focused on those changes in genome expression that are triggered by environmental factors. Examples of descriptive proteomes include the global comparison of green and etiolated rice shoots (13) and an analysis of defense-associated responses in the rice leaf and leaf sheath following a jasmonic acid treatment (16). One major advantage of the rice two-dimensional PAGE data base, in which most known proteins are recorded, is the wealth of new proteins on which experiments can be conducted at the biochemical and molecular levels.…”

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confidence: 99%

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Rice Proteomics

Komatsu

Konishi

Shen

et al. 2003

Molecular & Cellular Proteomics

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The technique of proteome analysis with two-dimensional PAGE has the power to monitor global changes that occur in the protein expression of tissues and organisms and/or expression that occurs under stresses. In this study, the catalogues of the rice proteome were constructed, and a functional characterization of some of these proteins was examined. Proteins extracted from tissues of rice and proteins extracted from rice under various kinds of stress were separated by two-dimensional PAGE. An image analyzer was used to reveal a total of 10,589 protein spots on 10 kinds of two-dimensional PAGE gels stained by Coomassie Brilliant Blue. The separated proteins were electroblotted onto a polyvinylidene difluoride membrane, and the N-terminal amino acid sequences of 272 of 905 proteins were determined. The internal amino acid sequences of 633 proteins were determined using a protein sequencer or mass spectrometry after enzyme digestion of the proteins. Finally, a data file of rice proteins that included information on amino acid sequences and sequence homologies was constructed. The major proteins involved in the growth and development of rice can be identified using the proteome approach. Some of these proteins, including a calcium-binding protein that turned out to be calreticulin and a gibberellin-binding protein, which is ribulose-1,5-bisphosphate carboxylase/oxygenase activase in rice, have functions in the signal transduction pathway. The information thus obtained from the rice proteome will be helpful in predicting the function of the unknown proteins and will aid in their molecular cloning.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Rice Proteomics

Komatsu

Konishi

Shen

et al. 2003

Molecular & Cellular Proteomics

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“…This suggests that the ratio of blocked proteins in wheat are signicantly lower compared to that observed in rice, in which only 2552z of Nter minal sequences were obtained. 11,12) The internal amino acid sequences of 8 protein spots (spot number #2, #3, #5, #11, #15, #18, #26, and #32) were disco vered by sequence analysis of PVDF membrane blotted polypeptides following the Cleveland peptide mapping with Staphylococcus aureus V8 protease. 10) The amino acid sequences of the above 56 protein spots were compared to the EST database of T. aesti vum using the TBLASTN program at DDBJ.…”

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confidence: 99%

“…11,12) Most of the lemma proteins identied in the proteome analysis were related to photosynthesis, which is similar to the result reported for rice leaf. 13,14) In addition, fragmentations of RuBisCo protein families were observed as in the case of mature rice leaf proteins.…”

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Proteome Analysis of Wheat Lemma

Woo

Kimura

Higa-Nishiyama

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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Plant Proteomics

Saravanan¹,

Bashir²,

Rose³

2004

Handbook of Plant Biotechnology

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Proteomics, or the study of the protein complement of the genome, promises to help bridge the divide between genomic DNA sequence and biological state and represents one of the most rapidly developing and innovative fields in genome‐scale research. The term is now commonly and broadly used to encompass almost any aspect of protein structure, expression and function. However, as used here it refers to characterising quantitative and qualitative characteristics of global protein expression, with an emphasis on scale, including polypeptide synthesis, degradation, post‐translational modification, the structure of protein complexes and interactions with other proteins and cellular components. The systematic characterisation of complex protein populations can be pursued using many approaches and numerous reviews have recently been published describing developments in diverse areas of proteomics, including protein structure, function and protein–protein, or protein–ligand interactions A number of reviews have also been specifically devoted to plant proteomics. Consequently, the multiplicity of proteomics‐related experimentation and developing technologies are not reiterated here in detail. Instead, this chapter intends to provide an overview of the diversity of proteomics‐based studies that have been reported in plants to date and emerging technologies, which in turn should underscore the tremendous potential utility of proteomics for plant biologists and give an indication of future applications.

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Separation and characterization of proteins from green and etiolated shoots of rice (Oryza sativaL.): Towards a rice proteome

Cited by 94 publications

References 14 publications

Rice Proteomics

Rice Proteomics

Proteome Analysis of Wheat Lemma

Plant Proteomics

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