Separation and Characterization of Soluble Adenovirus Type 9 Components

Norrby, Erling; Nyberg, Björn; Skaaret, P.; Lengyel, A

doi:10.1128/jvi.1.6.1101-1108.1967

Cited by 31 publications

(17 citation statements)

References 22 publications

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“…Listed in order of their elution from an anion exchanger by NaCl solutions of increasing molarity, these were (i) a type-specific antigen postulated to represent fibers, (ii) a group-specific antigen identified as hexons, and (iii) a dodecahedral aggregate of 12 pentons (2) carrying complete HA activity. Similar results were obtained in this laboratory in parallel studies of adenovirus type 9 (16). However, the latter study described in addition the occurrence of an incomplete HA postulated to represent isolated pentons.…”

supporting

confidence: 85%

“…Ultrastructural examinations of complete HA of types 15 and 9-15 demonstrated the type of star-shaped structures already demonstrated for some members of subgroup II, types 13, 15,19 (2), and 9 (16). This type of component, the dodecon, has been interpreted to be composed of 12 pentons plus some extra structure.…”

Section: Discussionmentioning

confidence: 57%

“…Tests for biological activities. Determination of complete and incomplete hemagglutinins and of hemagglutination-inhibition antibody-consuming (HIC) components was performed in tubes by use of the techniques previously described (8,14,16,17,27). Four hemagglutination-enhancing (HE) units of serum, determined in a chess-board titration, were applied per antigen dilution in tests for incomplete HA.…”

Section: Methodsmentioning

confidence: 99%

“…As an introduction to a study of these relationships on the level of structural antigens, experiments were designed for a separation and characterization of SOLUBLE COMPONENTS OF ADENOVIRUS TYPES soluble components of types 9, 15, and 9-15. Some different soluble components of type 9 already have been studied (16).…”

mentioning

confidence: 99%

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Comparative Studies on the Soluble Components of Adenovirus Types 9 and 15 and the Intermediate Strain 9-15

Norby

1968

J Virol

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Five different soluble components of adenovirus types 9, 9-15, and 15 have been identified. These are: (i) a slowly sedimenting, trypsin-resistant, incomplete hemagglutinin (HA). (This component was demonstrable by hemagglutination-enhancement (HE) tests in the presence of heterotypic antisera against members of Rosen's subgroups II and III, but not of subgroup I); (ii) a slowly sedimenting, trypsinresistant, complete HA, causing only a partial agglutination of cells; (iii) a rapidly sedimenting, incomplete HA, demonstrable by HE tests in the presence of heterotypic antisera against members of all Rosen's subgroups. (Trypsin treatment of this component caused a conversion into slowly sedimenting incomplete HA); (iv) a group-specific complement-fixing (CF) antigen devoid of HA activity; and (v) a rapidly sedimenting, trypsin-sensitive, complete HA, which in the electron microscope was found to represent a dodecahedral aggregate of 12 pentons (a dodecon). On the basis of their biological and physicochemical characteristics, the first four components were interpreted to represent (i) fibers, (ii) a polymer of a few, probably two, fibers, (iii) pentons, and (iv) hexons, respectively. The length of fibers extending from dodecons and virions was estimated to be 11 to 14 nm. A similar value was suggested from exclusion chromatography experiments. Adenovirus types 9 and 15 fibers were recovered in a position intermediate to that of fibers of types 3 and 4, the lengths of which are 10 and 17 nm, respectively. The sequence of elution of different components of types 9 and 9-15 from an anion exchanger was fibers, fiber-aggregate, pentons, hexons, and dodecons. Type 15 components appeared in the same order except for the fact that dodecons eluted before hexons. The molarities of NaCl required to elute the different types 9 and 9-15 components, excluding hexons, were identical. They were distinctly different from those of the corresponding type 15 components. However, hexons of all three serotypes eluted in proximity to each other and there was a slight tendency for type 9-15 hexons to take a position intermediate to those of types 9 and 15. The separation of human adenoviruses into 31 different serotypes is based on results of neutralization and hemagglutination-inhibition (HI) tests. However, neither one of these two tests is

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supporting

confidence: 85%

Section: Discussionmentioning

confidence: 57%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Comparative Studies on the Soluble Components of Adenovirus Types 9 and 15 and the Intermediate Strain 9-15

Norby

1968

J Virol

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“…Final purification of the dodecons was achieved by ion-exchange chromatography performed in columns (5 by 70 cm) packed with diethylaminoethyl (DEAE)-Sephadex A-25 resin (Pharmacia, New York, N.Y.) with tris(hydroxymethyl)aminomethane (Ttis)hydrochloride buffer (0.04 M, pH 8.4) as the equilibrating buffer (13). The column was loaded with 15 to 20 ml of concentrated, dialyzed, CaHPO4 eluate.…”

Section: Materuils and Methodsmentioning

confidence: 99%

Immunological Basis of the Adenovirus 8-9 Cross-Reaction

Hierholzer

Dowdle

1970

J Virol

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The dodecon and hexon components of adenovirus types 8 and 9 have been extensively purified for use in establishing the basis of the cross-reaction between these types. Dodecons, the complete hemagglutinins, were purified 304to 362-fold by fluorocarbon extraction, calcium phosphate batch chromatography, and ion-exchange column chromatography. Hexons, the group complement-fixation (CF) antigens, were purified 230to 240-fold by erythrocyte adsorption, ion-exchange chromatography, and exclusion chromatography. Component antisera prepared in rabbits were tested in reciprocal fashion with crude virus and dodecon and hexon components. By hemagglutination-inhibition (HI), the dodecons of types 8 and 9 demonstrated the same predominantly one-sided relationship characteristic of the crude antigens. Some neutralizing activity was associated with both dodecons and hexons of each type. However, combining anti-dodecon and anti-hexon sera or producing antisera against the combined dodecon-hexon components resulted in neutralizing titers which were identical to titers obtained with antisera against the crude virus harvests. Dodecons of each type appear to share at least one antigenic determinant with hexons of the same type, and this determinant may reside on the vertex capsomere. Hexons possess group-and type-specific determinants, as shown by CF, neutralization, and immunodiffusion tests, and may exhibit some minor relationship between types 8 and 9. The results with the purified components are consistent with the predominantly one-sided antigenic relationship between types 8 and 9 in the conventional HI tests and the largely type-specific relationship by neutralization tests.

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