Separation and determination synchronously by multichannel mode‐filtered light capillary electrochromatography

Abstract

Mode-filtered light capillary electrophoresis (CE) was developed for capillary electrochromatography (CEC) by applying annular columns of different size (250, 320, and 530 micro m) and a bovine serum albumin (BSA)-modified chiral stationary phase. BSA was encapsulated on the pretreated surface of an optic fiber and the inner wall of a capillary using sol-gel technique with a resultant larger active stationary phase area. The coating was examined by atomic force microscopy (AFM) and Fourier transform infrared (FT-IR). The separation of DL-tryptophan (10 mM) was investigated under different voltage/current conditions.

Strategy for Allosteric Analysis Based on Protein-Patterned Stationary Phase in Microfluidic Chip

“…The minimized nonspecific adsorption coupled with higher EOF also led to the efficient separations . Meanwhile, the addition of proper concentration of 2-propanol in the running buffer could improve the shape of peaks and inhibit the tailing phenomenon …”

“…38 Meanwhile, the addition of proper concentration of 2-propanol in the running buffer could improve the shape of peaks and inhibit the tailing phenomenon. 39 For comparison, the enantioselectivities on PMMA channel grafted alumina gel without BSA entrapped and untreated one were investigated. Only one peak in poor shape and band broadening was given out on untreated PMMA chip, which might attribute to the surface adsorption.…”

Mode-filtered light methane gas sensor based on cryptophane A

“…Comparatively, this type of sensors substantially increases the S/N. Since the inception of this sensing technique, it has been widely applied to various liquid samples, such as aromatic and chlorinated hydrocarbons [7][8][9][10], ethanol [11,12], glucose and glycerol [13], acetic acid [14], and amino acids [15,16]. So far, only a few gas samples were studied at steady-state concentrations by heated annular column [9].…”