Separation and evaluation of free radical‐scavenging activity of phenol components of Emblica officinalis extract by using an HPTLC–DPPH^• method

Abstract

A new procedure has been developed to separate and quantify the free radical-scavenging activity of individual compounds from an Emblica officinalis extract based on the combination of HPTLC with a diode array detector (DAD) and postchromatographic DPPH* radical derivatization. Free gallic and ellagic acids and emblicanins A and B in the E. officinalis extract were separated by TLC and identified. All the compounds of the extract were capable of scavenging of DPPH* radicals. It was established that the DPPH* scavenging activity of emblicanins A and B was 7.86 and 11.20 times more than that of ascorbic acid and 1.25 and 1.78 times more than gallic acid, respectively. From the estimated ID50 values, it can be seen that the increasing order of activity was emblicanin B > emblicanin A > gallic acid > ellagic acid > ascorbic acid. Probably, the antioxidant activity of E. officinalis extract is associated with the presence of hydrolyzable tannins having ascorbic acid-like action.

Hydroalcoholic extract ofEmblica officinalisprotects against kainic acid-induced status epilepticus in rats: Evidence for an antioxidant, anti-inflammatory, and neuroprotective intervention

“…Experimental studies have reported the potent antioxidant property of the fruit of E. officinalis (Yokozawa et al, 2007;Sharma et al, 2009). Various phytochemical constituents of the plant such as emblicanins A and B, gallic acid, ellagic acids have been identified as powerful free radical scavengers (Pozharitskaya et al, 2007). Moreover, other phytochemicals with NO scavenging properties such as geraniin, corilagin, and furosin have been reported in the E. officinalis fruit extract (Kumaran and Karunakaran, 2006).…”

“…The fruits are also of culinary use and contain thermostable vitamin C, minerals, and amino acids. It has high antioxidant activity on account of tannoids, tannins, vitamin C, and flavonoids (Pozharitskaya et al, 2007). The pharmacological studies on fruit of E. officinalis has revealed that it has good antioxidant, cytoprotective and immunomodulatory, cardioprotective, antiulcerogenic, antimutagenic, and hepatoprotective activity (Khan & Khan, 2009;Sharma et al, 2009;Kim et al, 2010).…”

Separation and evaluation of free radical‐scavenging activity of phenol components of green, brown, and black leaves of Bergenia crassifolia by using HPTLC‐DPPH• method

“…The evaluation of the total antioxidant activity of B. crassifolia extracts shows that this is due to the presence of a large percentage of phenols [3]. Consolidating chromatographic separation and antiradical activity determination allows analysts to evaluate the antioxidant contribution of each component within a total extract [11]. Furthermore, it allows the isolation of the most active compounds and decides upon the best technology to formulate products with these properties.…”

Protective Effect of Emblica officinalis Against Alcohol-Induced Hepatic Injury by Ameliorating Oxidative Stress in Rats

“…Phyllanthus emblica L.; Family: Euphorbiaceae) is regarded as ''one of the best rejuvenating herbs'' in the Ayurveda: an Indian traditional medicinal science. Emblica officinalis extract contains several antioxidants such as emblicanin A and B, gallic acid, ellagic acid, ascorbic acid that possesses strong antioxidative activity [7,8]. The fruit extract has many pharmacological activities for the treatment of a number of diseases and is a constituent of many hepatoprotective formulations [9].…”