Separation and identification of endoxylanases from Bacillus subtilis and their actions on wheat bran insoluble dietary fibre

Abstract

A novel and convenient method based on native polyacrylamide gel electrophoresis (PAGE) and homogenization extraction was used for the purification of xylanase from crude enzymes. Two xylanases were purified by this method from the crude enzyme preparation from the selected strain of Bacillus subtilis. Subsequent analysis with thin layer chromatography and high pressure liquid chromatography (HPLC) indicated that these two xylanases were endo-acting enzymes, designated xyl I and xyl II. Both enzymes showed their activities in the pH range from 5.0 to 9.0 at 50 8C and had similar optimum activities at pH 7.0 and at 50 8C. Mn 2+ ions enhanced their xylanolytic activities to 2.7-fold whereas Fe 3+ completely inhibited them. The action of endoxylanase xyl II on wheat bran insoluble dietary fibre was also studied. The hydrolysis products were shown to contain feruloyl oligosaccharides by paper chromatography. #

Cloning, Expression, and Purification of Xylanase Gene from Bacillus licheniformis for Use in Saccharification of Plant Biomass

“…Xylanase from Bacillus sp. SSP-0 was completely inhibited by Hg +2 [46], and in B. subtilis, Mn +2 ions enhanced xylanolytic activities by 2.7-fold, whereas Fe +3 completely inhibited the enzyme activity [47]. Khandeparker et al [48] observed that Fe +3 , Cu +2 , and EDTA inhibited the activity of the xylanase enzyme, while Ca +2 , Zn +2 , and Co +2 enhanced the xylanase activity.…”

Optimization of Xylanase Production byThermomyces lanuginosusin Solid State Fermentation

“…Its dry weight is comprised of approximately 25-40% xylan, making it an appropriate substrate for xylanase induction. 17,18) The present report details a stepwise approach to optimize a variety growth parameters associated with enzyme production. Due to the hyphal growth of filamentous fungi and its interaction with the substrate, it can be very difficult to quantify fungal mass in SSF directly.…”

Purification and Biochemical Characterization of Xylanases from Bacillus Pumilus and their Potential for Hydrolysis of Polysaccharides

“…Endoxylanase production by SSC expressed as U/g DBB (dry bacterial bran). Total soluble protein was estimated using bovine serum albumin as standard and also by direct method where the absorbance taken at 280 nm, the free Tyrosine (Tyr) and Tryptophan (Trp), uric acid, and bilirubin interfere at 280 nm [4].…”

“…Two xylanases from B. subtilis purified by native PAGE and homogenization extraction analyzed using HPLC. Both xylanase were endo acting, designated xyl I and xyl II (activity range pH 5.0 to 9.0 at 50 o C with optimum activities at pH -7 and 50 o C) [4]. A. versicolor produces a xylanolytic complex with two components, the minor component designated xylanase II (5 days in 1 % wheat bran) a monomeric glycoprotein with molecular mass of 32 kDa with 14.1 % of carbohydrate content.…”