Separation and purification of an anti-tumor peptide from rapeseed (Brassica campestris L.) and the effect on cell apoptosis

Abstract

Rapeseed peptides were prepared by means of the combined methods of the laboratory bacteria enzyme synergy and the solid-state fermentation of rapeseed meal. The rapeseed peptides were separated and purified with the tumor cell in vitro anti-proliferative activity as an index. Moreover, a kind of rapeseed peptide component RSP-4-3-3 (rapeseed anti-tumor peptide RSP-4-3-3) with high activity was selected. Furthermore, by using reversed-phase high performance liquid chromatography (RP-HPLC) coupled with electrospray ionization mass spectrometry (ESI-MS/MS), the analysis result of its possible amino acid sequence showed that it was Trp-Thr-Pro (408.2 Da). Inverted microscope observation technology and western blot experiments were applied to explore the antitumor impact of the rapeseed peptide RSP-4-3-3 on tumor cells. The results showed that the rapeseed antitumor peptide RSP-4-3-3 could significantly change the morphological features of the HepG2 cells in vitro and cause apoptosis, thus inhibiting the proliferation of the HepG2 cells.

Anticancer activity of protein fractions from chia (Salvia hispanica L.)

“…Also, Wang et al. (2016) from Brassica campestris identified a peptide (WTP), of 408.2 Da that at a concentration of 1.6 mg/ml and 72 hr of treatment, was able to reduce cell viability of HepG2 by 36.97 4.06%. On the HepG2 cell line, the percentage of inhibition reported by the <1 kDa fraction is higher than those reported by the single identified peptides discussed above; these differences in activity could be attributed to the characteristics of the peptides that compose the protein fraction, such as their amino acid composition, length, net charge, and structural conformation.…”

“…L. Wang et al. (2016) reported the separation and purification of a protein fraction with a molecular weight of 0.408 kDa from Brassica campestris , which induced apoptosis on the HepG‐2 cancer cell line. Luna‐Vital et al.…”

Oligopeptide derived from solid-state fermented cottonseed meal significantly affect the immunomodulatory in BALB/c mice treated with cyclophosphamide

“…Immunomodulatory peptides have attracted much attention because they can enhance the immunity of organisms, stimulate the proliferation of lymphocytes, increase the phagocytic activity of macrophages as well as defense against pathogens in organisms, promote the antibody production and regulate the transformation of T and B cells, and stimulate the secret of cytokine and immunoglobulin (Mallet et al, 2017 ). Recently, various bioactive peptides derived from enzymatic hydrolysis of soybean (Mallet et al, 2017 ), chickpea (Xue et al, 2015 ), rapeseed (Wang et al, 2016 ), wheat (Kawaguchi et al, 2017 ), rice (Taniguchi et al, 2017 ) has been shown interesting immunomodulatory ability.…”

Effect of Cu/Mn-Fortification on In Vitro Activities of the Peptic Hydrolysate of Bovine Lactoferrin against Human Gastric Cancer BGC-823 Cells

“…However, another anti-apoptotic protein Bcl-2 has a function to reduce cytochrome c release, which can be suppressed by p53 [38]. The peptides from rapeseed can up-regulate p53 and Bax but down-regulate Bcl-2 expression in HepG2 cells, while rice protein hydrolysates can induce H9c2 myocardiocytes apoptosis through the Bcl-2/Bax pathway [39,40]. More importantly, a previous study demonstrating a short-term cooperation of 3,4-dihydroxy-trans-stilbene and exogenous Cu also showed preferential apoptosis induction of HepG2 cells via mitochondria apoptosis pathway [41].…”