Separation and purification of chlorogenic acid by molecularly imprinted polymer monolithic stationary phase

Li, Hui; Liu, Yingju; Zhang, Zhaohui; Liao, Haiping; Nie, Lei; Yao, Shouzhuo

doi:10.1016/j.chroma.2005.08.046

Cited by 97 publications

(42 citation statements)

References 25 publications

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“…This is achieved by arranging functional monomers around a template compound and then fixing the monomers in this spatial arrangement with a crosslinker [1]. An ideal molecularly imprinted polymer (MIP) has homogeneous binding sites which show no co-operative properties and no matrix effects [2].…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of a lamotrigine imprinted polymer and its application for drug assay in human serum

Mohajeri¹,

Ebrahimi²

2008

J of Separation Science

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A molecularly imprinted polymer (MIP) against lamotrigine (LTG) was prepared, characterized, and its recognition properties were compared with a blank nonimprinted polymer (NIP). Two classes of binding sites were found in the MIP--high affinity (K(D) = 16.2 microM) and low affinity (K(D) = 161.3 microM). Selectivity of the synthesized MIP was examined using compounds with similar structures or therapeutic uses to LTG. In compounds which had structural similarity to LTG, the presence of amine groups appeared to affect binding to the MIP, however overall shape of the molecule was also important. Under the optimal conditions developed, other anticonvulsant drugs tested did not bind the MIP. A molecularly imprinted SPE (MISPE) procedure was developed which had a recovery of 84-89%, interday variation of less than 3.4% and intraday variation of less than 2.8%. The MISPE procedure was compared with a routine liquid-liquid extraction (LLE) procedure used for the HPLC determination of LTG in serum from patients. The data indicated that the MIP synthesized showed both good selectivity and high affinity for LTG and could be used for the extraction of the drug from serum samples or as the receptor layer for an LTG selective biosensor.

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Section: Introductionmentioning

confidence: 99%

Preparation and characterization of a lamotrigine imprinted polymer and its application for drug assay in human serum

Mohajeri¹,

Ebrahimi²

2008

J of Separation Science

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“…Separation of the corresponding enantiomers was achieved but the separation mechanism was not mentioned. In recent years, the uses of monolithic media for superior chromatographic separation in high-performance liquid chromatography and capillary electrochromatography have attracted considerable attention [94][95][96][97][98][99][100][101][102].…”

Section: Monolithic Imprinted Polymerizationmentioning

confidence: 99%

Characteristic and Synthetic Approach of Molecularly Imprinted Polymer

Yan

Row

2006

IJMS

462

231

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Abstract:Molecularly imprinted polymers (MIP) exhibiting high selectivity and affinity to the predetermined molecule (template) are now seeing a fast growing research. However, optimization of the imprinted products is difficult due to the fact that there are many variables to consider, some or all of which can potentially impact upon the chemical, morphological and molecular recognition properties of the imprinted materials. This review present a summary of the principal synthetic considerations pertaining to good practice in the polymerization aspects of molecular imprinting, and is primarily aimed at researcher familiar with molecular imprinting methods but with little or no prior experience in polymer synthesis. The synthesis, characteristic, effect of molecular recognition and different preparation methods of MIP in recent few years are discussed in this review, unsolved problems and possible developments of MIP were also been briefly discussed.

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“…It was prepared by one-step, in-situ polymerization 'molding' process directly within a chromatographic column without the tedious procedures of grinding, sieving and column packing. Compared with conventional particle columns, the monolithic columns possess a unique porous structure, and large through-pores exist in these monoliths, which enable mobile phases to flow through the adsorbents with low flow resistance at high flow rates (Li et al, 2005;Huang et al, 2004;Yan et al, 2005). As a technique for the creation of artificial receptor-like binding sites with a 'memory' for the shape and functional group positions of the template molecule, MIP has drawn much attention and has been used successfully in many fields of chemistry and biology, including chromatography (Hosoya et al, 1998), artificial antibodies (Ye and Mosbach, 2001), chemical sensors (Marx et al, 2004) and solid-phase extraction (Masquée et al, 2001;Jodlbauer et al, 2002;Qiao et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Novel molecularly imprinted monolithic column for selective on‐line extraction of ciprofloxacin from human urine

Yan

Row

2008

Biomedical Chromatography

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Water-compatible ciprofloxacin-imprinted monolithic columns were synthesized in water-containing systems for selective extraction of ciprofloxacin from human urine samples. Methanol-water (10:3, v/v) was used as a porogenic solvent and the obtained monolithic imprinted polymers reveal high selectivity to ciprofloxacin in an aqueous environment; the affinity can be easily controlled by adjusting the pH of the mobile phase. Owing to the unique porous structure and flow-through channels existing in the network skeleton of the monolithic MIP, urine samples could be directly injected into the column, proteins and other biological matrix were quickly washed out and ciprofloxacin was selectively retained and enriched. Good linearity was obtained from 0.08 to 400 mg/L (r=0.998) with the relative standard deviations less than 3.6%. The limit of detection of the method was 0.04 mg/L and the recoveries were more than 94.5% at three different concentrations. Moreover, by increasing the injection volume to 2.0 mL, the sensitivity of the method could be improved 100-fold according to the peak height of ciprofloxacin. This expedient greatly simplified the overall procedure, resulting in a rapid and efficient sample analysis while maintaining precision and accuracy.

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Separation and purification of chlorogenic acid by molecularly imprinted polymer monolithic stationary phase

Cited by 97 publications

References 25 publications

Preparation and characterization of a lamotrigine imprinted polymer and its application for drug assay in human serum

Preparation and characterization of a lamotrigine imprinted polymer and its application for drug assay in human serum

Characteristic and Synthetic Approach of Molecularly Imprinted Polymer

Novel molecularly imprinted monolithic column for selective on‐line extraction of ciprofloxacin from human urine

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