Separation and purification of lecithins by high pressure liquid chromatography

Abstract

Ten different synthetic lecithins have been analyzed by reverse‐phase high pressure liquid chromatography. An empirical lecithin “carbon number” that depends on the total number of carbons and double bonds in the fatty acyl chains in a useful index in predicting retention volumes of lecithins on a nonpolar octadecyl fatty acid column. Commercial egg lecithin is separated into its components by this technique.

The Lipids of Biological Membranes

“…Unsaturated fatty acids are esterified predominantly at position 2′ and the saturated fatty acids are found predominantly at position 1′ (Table 2.3). 6 This pattern of location of saturated and unsaturated fatty acids arises from a metabolic remodeling process. As described earlier, phospholipase A 2 cleaves at position 2′ of the glycerol.…”

High pressure liquid chromatographic separation of molecular species of phosphatidic acid dimethyl esters derived from phosphatidylcholine

“…1). As in the case with HPLC of egg yolk PC (6), better resolution of molecular species of "soy-PC" can be demonstrated by a comparison of the HPLC ehromatograms obtained for soy-PC-derived PAME on 2 Partisil-10 ODS columns in tandem ( Fig. 3) and for soy-PC on reverse phase 0a-Bondapak C-18), as described recently by Crawford et al (9).…”

Purification of phospholipids by preparative high pressure liquid chromatography

“…The chromatogram, after purification by preparative HPLC of 250 mg crude digalactosyldiglyceride from spinach (11,12), is shown in Figure 4. In run A (chromatogram given in the insert), fraction 3 was the only one containing digatactosyl-diglyceride.…”

“…High performance liquid chromatography (HPLC) has shown great potential for the separation of phospholipids on an analytical scale (1)(2)(3)(4)(5)(6)(7)(8)(9)(10). Limited information currently is available concerning the purification of phospholipids on a gram scale (11)(12). It would be of great practical use to develop HPLC equipment for the fast separation of lipids in large quantities (20 mg-5 g) using silica gel as a support for the stationary phase.…”

“…The pulse-damping system and the large preparative HPLC column used in the current study were developed because they were commercially unavailable. The choice of eluent for the separation of lipids is restricted to systems such as chloroform/methanol (6,11,12), hexane/isopropanol/water (9,10) and acetonitrile[ water (7,8). Lipid was detected in the hexane/ isopropanol/water and acetonitrile/water systems by monitoring the ~ absorption between 203 and 214 nm.…”