High pressure liquid chromatographic separation of molecular species of phosphatidic acid dimethyl esters derived from phosphatidylcholine

Hsieh, John Y.‐K.; Welch, David K.; Turcotte, Joseph G.

doi:10.1007/bf02535346

Cited by 28 publications

(1 citation statement)

References 9 publications

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“…Most phase systems used for PL separations have a mobile phase containing chloroform, dichloromethane (20,27), or acetonitrile (25,29,30) and are not compatible with the FPD detector. Gradient elution with a compatible mobile phase (31) using a silica column was not successful and would result in high detection limits.…”

Section: Resultsmentioning

confidence: 99%

Ultrasonic micronebulizer inferface for high-performance liquid chromatography with flame photometric detector

Karnicky¹,

Zitelli²,

Wal³

1987

Anal. Chem.

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An ultrasonic micronebulizer Interface for effective nebullzation at 2-20 juL/mln liquid was developed to allow the determination of nonvolatile analytes by microbore HPLC with virtually unmodified gas chromatographic detectors. A detectability of 50 pg/s phosphorus was obtained with a dynamic range of 3 decades and acceptable peak broadening using a flame photometric detector (FPD) as a detector. Solvent compatibility appears mainly determined by the choice of chromatographic detector. Dual-wavelength operation can Improve the slgnal-to-noise ratio by a factor of 5, Improve the precision from 6 to 2%, and eliminate base-line shifts. The utility of the mlcronebullzer-FPD as a selective HPLC detector Is demonstrated In the analysis of phospholipids and sugar phosphates.

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Section: Resultsmentioning

confidence: 99%

Ultrasonic micronebulizer inferface for high-performance liquid chromatography with flame photometric detector

Karnicky¹,

Zitelli²,

Wal³

1987

Anal. Chem.

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HPLC and Phospholipids Part I: General Considerations

Aitzetmüller

1984

Fette, Seifen, Anstrichm.

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Resolution of phospholipid molecular species by ³¹P NMR

Pearce

Komoroski

1993

Magnetic Resonance in Med

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Phosphorus-31 NMR chemical shifts of phospholipids (PLs) solubilized in bile salts were studied with respect to variations in the structure of the acyl substituents. The presence of double bonds in the acyl chains of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and phosphatidic acid (PA) induced small but consistent upfield shifts relative to the corresponding disaturated acyl PL signals. The magnitudes of the unsaturation-related upfield shifts were approximately twice as large in sodium cholate as in sodium deoxycholate. Chemical shift separations of PC, PE, and PG dipalmitoyl-dioleoyl species pairs increased slightly at lower temperatures. Resolution of the PC species pair was maximized and nearly independent of cholate-to-PL ratio at values greater than about 100 in 2% cholate. Only the PA species resolution varied significantly over the pH range 6.5 to 9.5, in the vicinity of its pK2. Shift differences for a homologous series of disaturated acyl PCs showed a logarithmic dependence on chain length. Spectra for a variety of PC standards were used to interpret the composite PC signals from egg yolk extract, soybean extract and whole human amniotic fluid. The 31P NMR analyses were consistent with published chromatographic studies. Interpretation of composite PL signals in the cholate system is simplified for mixtures having restricted acyl chain length and degree of unsaturation.

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High pressure liquid chromatographic separation of molecular species of phosphatidic acid dimethyl esters derived from phosphatidylcholine

Cited by 28 publications

References 9 publications

Ultrasonic micronebulizer inferface for high-performance liquid chromatography with flame photometric detector

Ultrasonic micronebulizer inferface for high-performance liquid chromatography with flame photometric detector

HPLC and Phospholipids Part I: General Considerations

Resolution of phospholipid molecular species by ³¹P NMR

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High pressure liquid chromatographic separation of molecular species of phosphatidic acid dimethyl esters derived from phosphatidylcholine

Cited by 28 publications

References 9 publications

Ultrasonic micronebulizer inferface for high-performance liquid chromatography with flame photometric detector

Ultrasonic micronebulizer inferface for high-performance liquid chromatography with flame photometric detector

HPLC and Phospholipids Part I: General Considerations

Resolution of phospholipid molecular species by 31P NMR

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Product

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Resolution of phospholipid molecular species by ³¹P NMR