HPLC and Phospholipids Part I: General Considerations

Aitzetmüller, K.

doi:10.1002/lipi.19840860806

Cited by 18 publications

(5 citation statements)

References 46 publications

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“…Among the various methods for the analysis of phospholipids ( − ), thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and 31 P nuclear magnetic resonance spectroscopy ( 31 P NMR) are the most important. Silica gel is typically used as stationary phase for TLC to separate phospholipid classes ( , ).…”

Section: Introductionmentioning

confidence: 99%

“…This problem has been solved by the use of the evaporative light scattering detector (ELSD), which can be used with all solvents and does not show any selectivity against the analyte (). Refractive index (RI) and flame ionization detectors (FID) have also been used ( , ). A forthcoming method is detection of phospholipid classes by mass spectrometry (MS) ( − ).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Methods for the Quantitative Determination of Phospholipids in Lecithins and Flour Improvers

Helmerich¹,

Koehler²

2003

J. Agric. Food Chem.

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Phospholipid classes were determined qualitatively and quantitatively in eight commercial lecithins and three flour improvers by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and (31)P nuclear magnetic resonance spectroscopy ((31)P NMR). The total amounts of phospholipids as well as the amounts of phospholipid classes in the samples were comparable but depended on the method used for quantification. Highest selectivity was provided by (31)P NMR as all phospholipids and lysophospholipids could easily be quantified. By TLC only lysophosphatidylcholine could not be quantified, whereas HPLC was the method with the lowest selectivity, because lysophospholipids, except lysophosphatidylethanolamine, could not be determined. Sensitivity was best for HPLC and TLC with detection limits of 20-170 mug/mL. By means of (31)P NMR these figures increased by a factor of 10-70. The coefficients of variation were 5.5, 6.8, and 12.8% for quantification by TLC, HPLC, and (31)P NMR, respectively, showing that TLC was the method with the best reproducibility. Altogether, (31)P NMR can be recommended for the quantification of phospholipids, because it is easy to perform and results can be obtained quickly. As it requires minimum instrumental equipment, TLC is a good alternative to (31)P NMR. If high sensitivity is required, HPLC is the best method.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of Methods for the Quantitative Determination of Phospholipids in Lecithins and Flour Improvers

Helmerich¹,

Koehler²

2003

J. Agric. Food Chem.

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“…The phospholipids are a group of polar lipids that are prevalent in nature. An excellent review of phospholipid (PL) analyses was recently published (19). The lack of a good, inexpensive detector is the main obstacle for PL analysis.…”

Section: Resultsmentioning

confidence: 99%

Ultrasonic micronebulizer inferface for high-performance liquid chromatography with flame photometric detector

Karnicky¹,

Zitelli²,

Wal³

1987

Anal. Chem.

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An ultrasonic micronebulizer Interface for effective nebullzation at 2-20 juL/mln liquid was developed to allow the determination of nonvolatile analytes by microbore HPLC with virtually unmodified gas chromatographic detectors. A detectability of 50 pg/s phosphorus was obtained with a dynamic range of 3 decades and acceptable peak broadening using a flame photometric detector (FPD) as a detector. Solvent compatibility appears mainly determined by the choice of chromatographic detector. Dual-wavelength operation can Improve the slgnal-to-noise ratio by a factor of 5, Improve the precision from 6 to 2%, and eliminate base-line shifts. The utility of the mlcronebullzer-FPD as a selective HPLC detector Is demonstrated In the analysis of phospholipids and sugar phosphates.

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“…These researchers attributed this phenomenon to the multiplicity of ionized forms exhibited by PA. The two broad peaks exhibited by PC may be due to the presence of multiple fatty acid species (17) and/or oxidized species of PC (18). Because of these peak shapes, quantitation by peak areas was difficult.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of self degumming properties of phospholipids in soybean oil using HPLC

Ragan¹,

Handel²

1985

J Americ Oil Chem Soc

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Freshly extracted crude soybean oil was obtained from a commercial soybean oil refinery, divided into aliquots, stored at 24 C and 5 C for varying lengths of time up to two weeks, and analyzed for total phosphorus. The concentrations of phosphatidylethanolamine (PE), phosphatidic acid (PA), phosphatidylinositol (PI) and phosphatidylcholine (PC) were determined by phosphorus analysis following separation by HPLC. Portions of the aliquots were degummed, and total phosphorus and individual phospholipids were determined as described above. Total phosphorus decreased 41% in crude oil stored at 24 C and 34% in crude oil stored at 5 C over two weeks. PE and PC precipitated more readily than PI and PA. Total phosphorus remaining in degummed oil was least after 12 hr storage of the crude oil and increased as the storage period became longer. The PE, PI and PA concentrations showed similar trends in degummed oil; however the PC concentration was least at 0 hr and increased thereafter. These results are discussed in terms of phospholipid‐phospholipid interactions and possible interactions between phospholipids and other compounds present in the oil.

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HPLC and Phospholipids Part I: General Considerations

Cited by 18 publications

References 46 publications

Comparison of Methods for the Quantitative Determination of Phospholipids in Lecithins and Flour Improvers

Comparison of Methods for the Quantitative Determination of Phospholipids in Lecithins and Flour Improvers

Ultrasonic micronebulizer inferface for high-performance liquid chromatography with flame photometric detector

Evaluation of self degumming properties of phospholipids in soybean oil using HPLC

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