Separation of peptides by pressurized capillary electrochromatography

Zhang, Kai; Jiang, Zhengjin; Yao, Chuanyi; Zhang, Zhichao; Wang, Qin‐Sun; Gao, Ruyu; Yan, Chao

doi:10.1016/s0021-9673(02)02063-0

Cited by 39 publications

(31 citation statements)

References 22 publications

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“…The effects of various parameters on the separation of an organic mixture of thiourea, benzyl alcohol, toluene, styrene, and naphthalene were also systematically investigated. The results obtained here may be useful to widen the research on CEC [13,17] and pCEC [27][28][29].…”

Section: Introductionmentioning

confidence: 74%

“…The lack of a fixed-volume injector in pure CEC is also a problem that hinders CEC from quantitative analysis. In order to overcome these problems and difficulties, pressurized CEC (pCEC) was developed [27].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the application of pressure in CEC provides a new operating parameter to the pCEC system, which allows a "fine-tuning" of the selectivity in a separation of charged and uncharged compounds by simply adjusting the ratio of voltage to pressure [30]. Solvent gradient capability and quantitative injection can be achieved when a binary solvent delivery system is used with a rotary type of injection valve [27].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Preparation and evaluation of C18‐bonded 1‐µm silica particles for pressurized capillary electrochromatography

Qu¹,

Xiao²,

Huang³

et al. 2006

Electrophoresis

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Nonporous silica spheres (1 microm) were synthesized and bonded with octadecylsilane functionality. These stationary phase particles were packed electrokinetically into fused-silica capillaries with 100 microm id for a length of 20 cm, which was evaluated by using pressurized CEC (pCEC). The efficiency of the C18 RP column was characterized through the theoretical plates of thiourea, benzyl alcohol, toluene, styrene, and naphthalene. The effects of experimental parameters such as the applied voltage, sample size, pump flow rate, pH value and the concentration of the buffer solution, and the content of methanol in the mobile phase, on-column efficiency were evaluated. Column efficiency as high as 200 000 theoretical plates per meter for naphthalene was obtained with the optimal condition of 70% v/v methanol and 30% v/v of 10 mmol/L phosphate buffer (pH 7.8) at an applied voltage of 10 kV and a supplementary pressure of 500 psi.

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Section: Introductionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Preparation and evaluation of C18‐bonded 1‐µm silica particles for pressurized capillary electrochromatography

Qu¹,

Xiao²,

Huang³

et al. 2006

Electrophoresis

Self Cite

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“…The experiments were performed on a laboratory-built pCEC system which was in accordance with the setup published in previous papers [16,18,21,22]. The AD was performed by using a home-made detection cell which was similar to previous reports [19] (see Fig.…”

Section: Pcec/admentioning

confidence: 99%

“…However, the intrinsic limitation of "pure" CEC, such as bubble formation and "dry-out" problem encountered with packed column, posted an obstacle to improve stability and reproducibility of experimental system [11]. By superimposing a pressure- induced hydrodynamic flow on an EOF in the electrochromatographic separation course [12], pressurized CEC (pCEC) could improve separation reliability and repeatability at the little expense of column efficiency, adjust the separation selectivity, increase the speed of separation, and avoid bubble formation [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Development of a new method for analysis of Sudan dyes by pressurized CEC with amperometric detection

Liu¹,

Zhang²,

Lin³

et al. 2007

Electrophoresis

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A new analytical method, pressurized CEC (pCEC) with amperometric detection (AD) using 1.5 mm RP nonporous silica packed columns has been developed for the rapid separation and determination of four Sudan dyes in hot chilli. The influence of several experimental parameters on the retention behavior has been investigated. The electrochemical oxidation of Sudans I-IV separated by pCEC can be reliably monitored with a carbon electrode at 10.95 V (vs. Ag/AgCl). Fast and efficient separation of the analytes was achieved within 7 min by pCEC under the optimum conditions with an ACN/water (95:5%) mobile phase containing formic acid (pH 4.3), 5% acetone and 0.002% triethylamine using a separation voltage of 12 kV. The detection limits for four Sudan dyes ranged from 8.0 6 10 27 to 1.2 6 10 26 mol/L. To evaluate the feasibility and reliability of this method, the proposed pCEC-AD method was further demonstrated with hot chilli samples spiked with Sudan dyes.

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Capillary Electrochromatography

Zhang

Gao

2015

Analytical Separation Science

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Capillary electrochromatography ( CEC ) is a hybrid microseparation technique that combines the high selectivity of high‐performance liquid chromatography ( HPLC ) and the high efficiency of capillary electrophoresis ( CE ). CEC holds unique advantages such as high separation power, high selectivity, high sensitivity, short analysis time, low consumption of samples and chemicals, and good compatibility with mass spectrometry ( MS ). Accordingly, it has attracted extensive attentions over the past decades. Recent progresses in CEC , especially the development of CEC stationary phases, such as fused silica‐packed columns, open tubular coating columns, monolithic columns, and molecular imprinted polymer columns, as well as the application of CEC in chiral separation and peptide/protein analysis, are summarized in this chapter.

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Separation of peptides by pressurized capillary electrochromatography

Cited by 39 publications

References 22 publications

Preparation and evaluation of C18‐bonded 1‐µm silica particles for pressurized capillary electrochromatography

Preparation and evaluation of C18‐bonded 1‐µm silica particles for pressurized capillary electrochromatography

Development of a new method for analysis of Sudan dyes by pressurized CEC with amperometric detection

Capillary Electrochromatography

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