Separation of plant pigments by thin layer chromatography

Abstract

Describes a lab activity to separate chloroplast pigments over a thin layer of silicic acid.

Modifications of solution chromatography illustrated with chloroplast pigments

“…On layers of silica gel G spread on glass plates and developed with the same solvent, the same pigments separate but in a different sequence: the zone of lutein is behind both chlorophylls rather than between them as shown in the figure. There is no indication of the extra yellow or green zones reported by Anwar (16) and Rollins (17) in earlier papers in this Journal dealing with the tic of chloroplast pigments on layers of siliceous adsorbents.…”

Pigments from some marine specimens. The isolation and spectral characterization of spinochromes

“…The hue of these spines are varied and may range from dark black, to red, to greenish white depending on the species. 1 These marine animals can normally be found on rocks and in the water along the intertidal zones.…”

“…popular experiments are the extraction of pigments from plants (1)(2)(3), caffeine from tea leaves (4), cholesterol from bile stones (5), and chitin and glucosamine from the shells of Crustacea (6). A complilation of selected experiments involving the isolation and characterization of natural products has become recently available in a laboratory text (f>).…”

Identificação de pigmentos naturais de espécies vegetais utilizando-se cromatografia em papel

“…O ensino dos princípios de tais técnicas pode ser feito usando-se várias fases estacionárias (papéis de filtro 2 , giz 3 , areia 4 , etc.) e diversas amostras, tais como: pigmentos de tecidos vegetais 5 , extratos de frutas 6 , pigmentos naturais 7 , tecidos vegetais clorofilados 8 . Dentre as várias técnicas cromatográficas, aquela com maior potencialidade didática em cursos básicos de química é a cromatografia em papel, devido à sua simplicidade, facilidade de execução e possibilidade de uso de amostras coloridas, em pequenas quantidades.…”