Separation of Sulfisoxazole, Phenazopyridine, and Their Related Impurities by Micellar Electro-Kinetlc Chromatography

Nickerson, Beverly; Scypinski, Stephen; Sokoloff, Helen; Sahota, Swroop

doi:10.1080/10826079508014629

Cited by 13 publications

(5 citation statements)

References 17 publications

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“…Both sulfanilamide and sulfanilic acid yielded linear responses from the detection limit of 0.25 pg/mL to the highest concentration examined, 20 pg/mL. When a 1.0 mg/mL sulfisoxazole solution was used for impurity quantitation, the detection limit represented 0.025 O/ o with respect to the active [38].…”

Section: Polypeptidesmentioning

confidence: 94%

“…An MEKC method was developed to analyze sulfisoxazole and phenazopyridine in a tablet dosage form [38]. The standard sulfisoxazole was resolved from possible degradation products sulfanilamide and sulfanilic acid, as well as from phenazopyridine and its impurities.…”

Section: Polypeptidesmentioning

confidence: 99%

“…4) [38]. Two samples of a tablet composite were assayed by MEKC at 96.5% and 94.2% of label claim, whereas a UV spectrophotometric assay found 104.4% sulfisoxazole [38].…”

Section: Tabletsmentioning

confidence: 99%

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Analysis of antibiotics by capillary electrophoresis

Flurer¹

1997

Electrophoresis

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The broad category of antibiotics encompasses some of the most widely prescribed pharmaceuticals in the world. As is the case with any pharmaceutical, an antibiotic must be characterized in terms of its potency or activity, and the presence and quantity of impurities. Additionally, any residue or metabolite that may be present as a result of its use must be monitored. Many capillary electrophoretic techniques have been utilized in the analysis of antibiotics, addressing the various aspects of their quantitation, profiling, and monitoring. Some of the more recent applications are summarized in this review article.

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Section: Polypeptidesmentioning

confidence: 94%

Section: Polypeptidesmentioning

confidence: 99%

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Analysis of antibiotics by capillary electrophoresis

Flurer¹

1997

Electrophoresis

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“…TMP was determined through HPLC in the presence of its decomposition products [10]. The separation of sulfonamides in the presence of their decomposition products was not possible through HPLC as the elution of sulfanilamide (SAM) and sulfanilic acid (SAA) takes place at or near the void volume, causing problems with their identification and quantification [11].…”

Section: Introductionmentioning

confidence: 99%

Assessment of MEKC suitability for residue drug monitoring on pharmaceutical manufacturing equipment

Boca¹,

Pretorius²,

Kgaje³

et al. 2008

Journal of Pharmaceutical and Biomedical Analysis

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The suitability of micellar electrokinetic chromatography for the simultaneous trace determination of several compounds (sulfamethoxazole, trimethoprim, sulfanilic acid, sulfanilamide, 3,4,5-trimethoxybenzoic acid and nonoxynol-9) was assessed. The mixture was separated within 14 min at an applied voltage of 22 kV by using 30 mM phosphate electrolyte, containing 10 mM SDS, adjusted to pH 7.8. Under optimized separation conditions acceptable levels of linearity, precision and accuracy were obtained for all compounds. The method could be used as part of a cleaning validation study when assaying trace levels of co-trimoxazole drug, some of its decomposition products and detergent in the swab samples collected from pharmaceutical manufacturing equipment, after cleaning.

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“…22,23 However, these methods are tedious and time consuming. The use of micellar electrokinetic chromatography for the separation of sulfonamides and related impurities has been reported by Nickerson et al 24 using UV detection. To the best of our knowledge, no reports of the electrochemical detection of SDZ and SMZ by CE have been published.…”

Section: Introductionmentioning

confidence: 99%

Determination of sulfadiazine and sulfamethoxazole by capillary electrophoresis with end-column electrochemical detection

You¹,

Yang²,

Wang³

1998

Analyst

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Capillary electrophoresis (CE) with end-column electrochemical detection (EC) of sulfadiazine (SDZ) and sulfamethoxazole (SMZ) is described. Under the optimum conditions, SDZ and SMZ were separated satisfactorily, and a highly sensitive and stable response was obtained at a potential of 1.1 V versus Ag/AgCl. Optimized end-column detection provides detection limits as low as 0.1 microM for both compounds, which corresponds to 0.024 and 0.021 fmol with peak efficiencies of 394,000 and 335,000 theoretical plates for SDZ and SMZ, respectively. The calibration graph was linear over three order of magnitude. The relative standard deviations (n = 12) of peak currents and migration times were 2.3 and 2.7%, and 0.8 and 1.3%, respectively, for the two compounds. The proposed method was applied to the analysis of tablets and human urine samples with satisfactory results.

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Separation of Sulfisoxazole, Phenazopyridine, and Their Related Impurities by Micellar Electro-Kinetlc Chromatography

Cited by 13 publications

References 17 publications

Analysis of antibiotics by capillary electrophoresis

Analysis of antibiotics by capillary electrophoresis

Assessment of MEKC suitability for residue drug monitoring on pharmaceutical manufacturing equipment

Determination of sulfadiazine and sulfamethoxazole by capillary electrophoresis with end-column electrochemical detection

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