Separation of β-agonists in pork on a weak cation exchange column by HPLC with fluorescence detection

Synthesis and application of boronic acid‐functionalized magnetic adsorbent for sensitive analysis of salbutamol residues in pig tissues

“…However, this drug is often abused as feed additive in graziing animals, as it acts as a growth promoter to effectively facilitate the catabolism of body fat and the production of muscle tissue. A study also found that the abuse of SAL can cause some adverse physiological responses for human beings (Meng et al ., ). The common physiological toxic reactions are hand or muscle tremor, nausea, arrhythmia, dizziness, weakness of limbs, headache, insomnia and emesis; it is more harmful to heart disease and high blood pressure patients.…”

“…A number of sample pre‐treatment methods, based on novel adsorbents, have been developed for the analysis of SAL. C 18 ‐SPE (Fiori et al ., ), weak cation exchange columns (Meng et al ., ), molecularly imprinted polymers (Yan et al ., ), polymer microextraction (Huang et al ., ; Du et al ., ), immunoaffinity columns (Wang et al ., ), tert ‐butylmethylether extraction (Garrido et al ., 2013) and poly (MAA‐EGDMA) SPE columns (Wang et al ., ) have been used to extract SAL from the complex biological samples. However, most of these methods are nonspecific and may lead to nonspecific adsorption from the sample matrix.…”

Broad screening and identification of β-agonists in feed and animal body fluid and tissues using ultra-high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry combined with spectra library search

“…These methods include liquid chromatography (Chang, Chou, & Hwang, 2005;Meng et al, 2012;Turberg, Macy, Lewis, & Coleman, 1994), gas chromatography (Bocca, Fiori, Cartoni, & Brambilla, 2003), liquid chromatography tandem mass spectrometry (LC-MS/MS) (Shishani, Chai, Jamokha, Aznar, & Hoffman, 2003;Wu, Liu, & Peng, 2014), gas chromatography-mass spectrometry (Montrade, Lebizec, Monteau, & Andre, 1995;Wang, Li, Zhang, & Shen, 2006), enzyme-linked immunoassay (Hahnau & Julicher, 1996;Harkins, Woods, Lehner, Fisher, & Tobin, 2001;Pinheiro et al, 2008), colloidal gold test strip (Khamta, Pattarawarapan, Nangola, & Tayapiwatana, 2009;Zhang et al, 2009), surface plasmon resonance immunosensors (Traynor, Crooks, Bowers, & Elliott, 2003), and electrochemical immunosensors (He, Wang, Zhang, & Yang, 2009). Table 1 Chemical formula, accurate mass, retention time and characteristic fragment ions of 32 b-agonists.…”

An isocratic solvent-free mobile phase HPLC-PDA analysis of clenbuterol and ractopamine

“…The optimal harmonized method must be easy-to-use, economical in time and cost, and must cause no harm to the environment and analyst. Although several high-performance liquid chromatographic (HPLC) methods have been developed for monitoring b-agonists with different detection modes such as UV, 17,18 fluorescence, 17 photodiode array (PDA), 19 mass spectrometry (MS), 20 and tandem mass spectrometry (MS/ MS), 21e25 these methods have crucial drawbacks: 1) all of the methods consume large quantities of organic solvents in the mobile phases. Risk associated with these solvents extend beyond direct implications for the health of humans and wildlife to affect our environment and the ecosystem in which we all reside.…”