Sepharose-linked concanavalin A in the purification and characterization of glycoprotein hormones of the bovine pituitary

Bloomfield, Glenys A.; Faith, Michael R.; Pierce, John G.

doi:10.1016/0005-2795(78)90383-5

Cited by 38 publications

(7 citation statements)

References 23 publications

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“…Multiple forms of FSH have now been demonstrated within anterior pituitary extracts of rats (Wakabayashi, 1977), man (Reichert, 1971;Sherins et al 1973;Zaidi et al 1981), monkeys , sheep (Sherwood, Grimek & McShan, 1970;Reichert, 1971) and cattle (Reichert, 1971;Bloomfield, Faith & Pierce, 1978). In addition, pituitary LH and human chorionic gonadotrophin also appear to exist in a variety of physicochemical forms Robertson et al 1977;Wakabayashi, 1977;Nwokoro et al 1981;Reddy & Menon, 1981).…”

Section: Discussionmentioning

confidence: 92%

Multiple species of follicle-stimulating hormone exist within the anterior pituitary gland of male golden hamsters

Ulloa‐Aguirre¹,

Chappel²

1982

Journal of Endocrinology

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Anterior pituitary glands were collected from immature and mature (intact and castrated) male hamsters. The various species of FSH present within these glands were separated by Concanavalin A (Con A) chromatography and polyacrylamide gel isoelectric focusing (PAG-IEF) and measured by a specific FSH radioimmunoassay (RIA) as well as a radioreceptor assay (RIA). Two distinct forms of FSH (Con A unbound and bound) were separated by Con A chromatography and detected by both RIA and RRA. These two populations of FSH were present within anterior pituitary glands of all three animal models tested. Castration before collection of anterior pituitary glands reduced the ratio of Con A unbound: bound immunoreactive FSH. When measured by RRA this reduction was no observed. When homogenates of anterior pituitary glands obtained from mature animals were separated by PAG-IEF, six distinct species of FSH were observed by RIA with isoelectric points (pI) of 6.0, 5.7, 5.3, 5.0, 4.7 and 4.2-3.8. Homogenates of anterior pituitary glands obtained from immature male hamsters did not contain one of these species of FSH (pI values 4.7). The relative contribution of some of the species of FSH to the total amount of detectable FSH differed depending upon the endocrine status of the animal. The species with pI value of 4.2-3.8 did not show any receptor-binding activity in any of the three models studied. The overall ratio of the activity of FSH measured by RRA compared with RIA was highest in anterior pituitary glands from intact mature and immature hamsters and lowest in anterior pituitary glands obtained from castrated animals. The RRA:RIA ratio for each species of FSH in all models tested declined as the isoelectric point of that species decreased. Thus, these results demonstrated the presence of multiple species of FSH within the anterior pituitary of immature and mature male hamsters. The relative proportions and receptor-binding activities of these species differed according to the isoelectric point and the pattern of hormone secretion at the time of collection of pituitary glands. Gonadal and other endocrine factors may influence not only the relative amount of each species of FSH but also the receptor-binding capacity of the FSH species synthesized by the anterior pituitary gland.

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Section: Discussionmentioning

confidence: 92%

Multiple species of follicle-stimulating hormone exist within the anterior pituitary gland of male golden hamsters

Ulloa‐Aguirre¹,

Chappel²

1982

Journal of Endocrinology

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“…1. Sepharose or agarose particles functionalized with either ConA, WGA or boronic acids have previously been applied for the successful isolation of glycosylated proteins [7][8][9][10]. The use of magnetic particles as support for the functionalization allows for easy miniaturization and automation of assays and thereby facilitating high through-put analyses.…”

Section: Introductionmentioning

confidence: 99%

Exploring the binding profiles of ConA, boronic acid and WGA by MALDI-TOF/TOF MS and magnetic particles☆

Sparbier

Wenzel

Kostrzewa

2006

Journal of Chromatography B

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“…In later years, pituitaries collected from abattoirs have been used more frequently [4]. The purification procedures for LH have relied upon multistep protocols involving precipitation, ion exchange chromatography and gel filtration [1,[4][5][6][7][8]. For example, a purified LH preparation from bovine pituitaries was obtained by salt precipitation, pH precipitations, gel filtration, ion exchange chromatography and a lectin column [5].…”

Section: Introductionmentioning

confidence: 99%

“…The purification procedures for LH have relied upon multistep protocols involving precipitation, ion exchange chromatography and gel filtration [1,[4][5][6][7][8]. For example, a purified LH preparation from bovine pituitaries was obtained by salt precipitation, pH precipitations, gel filtration, ion exchange chromatography and a lectin column [5]. The classical protocol for isolation of buffalo LH from pituitaries consists of seven steps which utilize pH precipitation, salt fractionation, ion exchange chromatography and gel filtration [8].…”

Section: Introductionmentioning

confidence: 99%

Purification of bubaline luteinizing hormone by gel filtration chromatography in the presence of blue dextran

Chaudhary

Jain

Muralidhar

et al. 2006

Process Biochemistry

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Sepharose-linked concanavalin A in the purification and characterization of glycoprotein hormones of the bovine pituitary

Cited by 38 publications

References 23 publications

Multiple species of follicle-stimulating hormone exist within the anterior pituitary gland of male golden hamsters

Multiple species of follicle-stimulating hormone exist within the anterior pituitary gland of male golden hamsters

Exploring the binding profiles of ConA, boronic acid and WGA by MALDI-TOF/TOF MS and magnetic particles☆

Purification of bubaline luteinizing hormone by gel filtration chromatography in the presence of blue dextran

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