Sequence analysis and population data of short tandem repeat polymorphisms at loci D8S639 and D11S488

Abstract

Short tandem repeat loci are ideal markers for forensic and paternity case work. A high degree of polymorphism, as determined by gross length measurement, is very often due to complex underlying sequence variation. In the present study, we have studied the sequence structure and population genetics of two short tandem repeat polymorphisms at loci D8S639 and D11S488 in German Caucasians from the region of Hesse. Sequence data revealed a considerable polymorphism at both loci. Locus D8S639 is characterized by a tetranucleotide (AGAT)n repeat pattern with (GAT) and (AGGT) repeats dispersed throughout several alleles. These microvariations lead to alleles differing by one base pair or alleles of identical size. At locus D8S639 we observed 17 allelic lengths comprising 25 different alleles. Alleles at locus D11S488 possess a compound repeat region consisting of (AAAG)n and (GAAG)n repeats. At locus D11S488 we observed 15 allelic lengths with a total of 24 alleles. Allelic lengths increased in size by 4bp increments corresponding to the addition of one tetranucleotide repeat unit. Population data of loci D8S639 and D11S488 revealed a high polymorphism with heterozygosity rates of 0.85 (D8S639) and 0.91 (D11S488).

Quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection: the Frankfurt experience

“…1,3,4,[8][9][10][11] Determination of donor chimerism should lead to comparable results in different laboratories, which implies that results ought to be independent of the STR primers and the detection system used. We compared a singleplex in-house STR primer set PCR 12 (Table 1) with two commercially available multiplex PCR systems (AmpFISTER Profiler, [5][6][7]13,14 AmpFISTER SGM Plus PCR amplification kit; 7,15 Applied Biosystems/ABI/, Weiterstadt, Germany) in a patient with thalassemia. PCR products were assessed by both capillary electrophoresis (ABI Prism 310 genetic analyzer) and polyacrylamide gel electrophoresis (PAGE) systems (ABI Prism 373 DNA sequencer).…”

Evaluation of an extended set of 15 candidate STR loci for paternity and kinship analysis in an Austrian population sample

“…After careful consideration, the following 15 markers were taken from the literature: D1S1656 [11][12][13], D7S1517 [14], D8S306 [15,16], D8S639 [17,18], D9S304 [19], D10S2325 [12,20], D11S488 [18,21], D12S391 [22][23][24][25], D14S608 [2,26], D16S3253 [26], D17S976 [27], D18S1270 [26], D19S253 [28], D20S161 [29], and D21S1437 [26]. The chromosomal locations of these 15 loci, as well as those of STR markers used in commercially available PCR multiplexes (e.g., Powerplex 16 system), were determined by searching the May 2004 human genome assembly using BLAT [30] (http://www.genome.…”

Older patients with high‐risk fungal infections can be successfully allografted using non‐myeloablative conditioning in combination with intensified supportive care regimens

“…Remission status and chimaerism Bone marrow was aspirated monthly to assess remission status using cytology and cytogenetics, and chimaerism using polymerase chain reaction (PCR)‐based assays analysing polymorphic short tandem repeat (STR) markers (Blau et al , 1999; Seidl et al , 1999; Thiede et al , 1999). Donor lymphocyte infusions were given to patient 1 on d +78 and d +92 (1 × 10 6 and 5 × 10 6 CD3 + cells/kg).…”