Sequence analysis of six full-length bean yellow mosaic virus genomes reveals phylogenetic diversity in India strains, suggesting subdivision of phylogenetic group-IV

Kaur, Charanjeet; Raj, Rashmi; Srivastava, Ashish; Kumar, Susheel; Raj, S. K.

doi:10.1007/s00705-017-3609-5

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…In general, phylogenetic studies, based on the coat protein sequence of BYMV, established the existence of seven groups, a general one, with a wide hosts range, and six others named according to the original hosts (broad bean, canna, lupine, monocot , pea and W) (Wylie et al 2008). Further analysis based on the entire virus genome, revealed the presence of nine distinct groups, including the subdivision of the former general group into three new ones (Kehoe et al 2014), and recently Kaur et al (2018) suggested that the General group IV of the previous classification could be divided into two subgroups (IVa and IVb ). In this scenario, we generated additional phylogenetic analyses to further assess evolutionary relations of the detected virus among BYMV.…”

Section: Resultsmentioning

confidence: 99%

Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina

Pardina

Nome

Reyna

et al. 2019

Preprint

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Broad bean (Vicia faba L) is the fourth most important pulse crop in the world. In Argentina, broad bean production was of 1,841 hectares and 16,500 tons during the 2017 growing season. Broad bean is commonly used in rotations; especially by farmers located in "green belts" that are peri-urban areas surrounding large cities that include horticultural family farms. Plants showing marked foliar mosaic symptoms, typical of viral infection, were collected during the 2015 growing season in the green belt of Córdoba city, Argentina. Preparations of symptomatic tissues were mechanically inoculated onto healthy broad bean plants in the greenhouse, which developed symptoms similar to those observed in the field. In addition, symptomatic samples were positive when tested by indirect ELISA with the anti-potyvirus group monoclonal antibody. Further, flexuous filamentous particles typical of potyviruses were observed under the electronic microscope on dip preparations. Lastly, total RNA was extracted from a symptomatic leaf and high-throughput sequenced, which allowed the assembly of a single virus sequence corresponding to a new highly divergent strain of Bean yellow mosaic virus (BYMV). Phylogenetic insights clustered this Argentinean broad bean isolate (BYMV-ARGbb) within group IX of BYMV. Given the economical importance of this virus and its associated disease, the results presented here are a pivotal first step oriented to explore the eventual incidence and epidemiological parameters of BYMV in broad bean in Argentina.

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Section: Resultsmentioning

confidence: 99%

Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina

Pardina

Nome

Reyna

et al. 2019

Preprint

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“…Tissue culture has improved gladiolus in many ways, including somaclonal variation and genetic stability [51,185], disease-free planting material selection in vitro [186][187][188], protoplast and somatic hybridization [189,190], synthetic seed technology [191,192], and in vitro mutagenesis [193]. Genetic transformation has been used in a number of reports to create transgenic gladiolus plants [24,[194][195][196].…”

Section: Insights and New Technologies For In Vitro Culture Of Gladiolusmentioning

confidence: 99%

In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): A Systematic Review Analysis Based on More Than 50 Years Research

Kumar,

Chaudhary,

Sirohi

et al. 2024

Horticulturae

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Traditional gladiolus propagation methods are now supplemented with in vitro propagation to meet the demands of modern floriculture in terms of quick production of disease-free, quality planting material. Due to virus infections, vegetative propagation in gladiolus in the field is slow, and is a serious concern in the propagation of gladiolus. In vitro propagation provides an enormous increase in propagation rate and the ability to produce disease-free plant material. Numerous elements, including cultivars, explant type, size of explants, position of explants on medium, plant growth regulators and certain additives, incubation conditions, and sub-culturing time, all have a significant impact on in vitro clonal propagation of gladiolus plants as well as the development of in vitro cormel efficiency. There are certain obstacles and challenges that arise in the in vitro development of plants and the cormels of gladiolus. However, numerous studies and review reports on gladiolus for in vitro propagation have been reported, but very little is known about the factors influencing gladiolus’ in vitro effectiveness. In the present review, we focused on and analyzed research data accumulated over 50 years on diverse strategies for in vitro propagation such as direct, indirect organogenesis, and somatic embryogenesis, as well as various factors such as physical, nutritional, and hormonal influences on in vitro propagation, in vitro cormel formation efficiency, difficulties that arise, and new insights into in vitro development in gladiolus from the available literature worldwide. Future possibilities for further improvement in the in vitro propagation of ornamental gladiolus are also discussed. The current review provides insight into a comprehensive protocol for gladiolus in vitro propagation and emphasizes the importance of continuously advancing tissue culture techniques and factors influencing the in vitro efficiency towards improving in vitro plantlets and cormels in gladiolus (Gladiolus spp.).

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“…On the contrary, isolates in the general group were identified in wild and domesticated hosts of monocots and dicots [3]. Some members of the monocot group can infect dicotyledonous species from three families: Orchidaceae, Iridaceae, Gentianaceae [5][6][7][8][9]. As phylogenetic relationships among BYMV isolates correlate with their natural hosts, Wylie et al (2008) proposed seven BYMV phylogenetic groupings based on coat protein sequences and the original hosts of the isolates: (i) general group with a broad host range of monocots and dicots; (ii) broad bean group; (iii) canna group; (iv) lupine group; (v) monocot group; (vi) pea group; and (vii) one strain 'W' from L. albus [3].…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Analysis of Bean Yellow Mosaic Virus Isolates From Gladioli in Ukraine

Dunich¹,

Sovinska²,

Dashchenko³

et al. 2022

BTSNUKSB

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Bean yellow mosaic virus (BYMV) is the one of most prevalent and harmful viruses infecting gladiolus plants worldwide. The aim of the study was to perform phylogenetic analysis of two Ukrainian BYMV isolates from gladioli grown in different regions of Ukraine and determine phylogenetic relationships of the isolates to other BYMVs. Visual diagnostics, enzyme-linked immunosorbent assay in DAS-ELISA modification, total RNA extraction, RT-PCR, phylogenetic analysis and statistical data analysis were used in this research. The results of the studies showed that gladioli of variety Pamyat’ (Poltava region, 2018) and var. Galyna Zelenobirska (Kyiv region, 2020) with symptoms of chlorotic stripes and mosaics on the leaves, flower color breaks are infected by BYMV. Fragments of the CP gene sequence of Ukrainian gladiolus isolates of bean yellow mosaic virus, named GlMP-18 (MK416160) and BYMV-Gl-SV-20 (MZ286966) were sequenced and deposited to the NCBI GenBank. Nucleotide sequences of these isolates corresponding to 578 nt of the coat protein gene (CP) located at the position 8727–9305 of the viral genome and amino acid sequences were compared with 40 known BYMV isolates/strains. Phylogenetic analysis demonstrated that GlMP-18 and BYMV-Gl-SV-20 have identity of nucleotide sequence 100% and amino acid sequence 100% with each other. Both Ukrainian isolates clustered with the Monocot group. The identity of the СР gene sequences of the two gladiolus isolates GlMP-18 and BYMV-Gl-SV-20 from geographically remote regions of the country and in different years of selection indicates a common origin of isolates and probable their distribution of planting material.

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Sequence analysis of six full-length bean yellow mosaic virus genomes reveals phylogenetic diversity in India strains, suggesting subdivision of phylogenetic group-IV

Cited by 5 publications

References 24 publications

Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina

Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina

In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): A Systematic Review Analysis Based on More Than 50 Years Research

Phylogenetic Analysis of Bean Yellow Mosaic Virus Isolates From Gladioli in Ukraine

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