Sequence analysis of the cytochrome c oxidase subunit 1 gene of Sarcoptes scabiei isolated from goats and rabbits in East Java, Indonesia

Abstract

Aim: This study aimed to sequence the Cytochrome c oxidase (COX-1) gene sequence from mitochondrial DNA of Sarcoptes scabiei isolated from Lamongan goats and Mojokerto rabbits, align it with DNA isolated from Zi'gong rabbit (GenBank accession No. EU256389.1), and produce a phylogenetic analysis of S. scabiei COX-1 gene. Materials and Methods: S. scabiei mites were obtained from goats and rabbits, and DNA was extracted using QIAamp DNA Mini Kit. The forward and reverse primer sequences were designed based on the DNA sequence of an S. scabiei COX-1 gene isolated from the Zi'gong rabbit (5'-TCTTAGGGGCTGGATTTAGTATG-3' and 5'-AGTTCCTCTACCAGTTCCAC-3', respectively). To confirm sequencing output, the sequence resulting from the reverse primer was inverted and aligned to the sequence from the forward primer using Clone Manager Professional Version 9 for Windows (Scientific & Educational Software; http://www.scied.com). This alignment was subsequently used to build a phylogenetic tree, using the Neighbor- Joining method, in the MEGA6 program (https://www.megasoftware.net/). Results: Polymerase chain reaction (PCR) products from S. scabiei isolates from Lamongan goats and Mojokerto rabbits produced bands of around 290 bp with 2% agarose gel electrophoresis. Comparing the DNA sequences of the S. scabiei COX-1 gene with those isolated from Lamongan goats and Mojokerto rabbits showed 99% homology. Conclusion: PCR products of the S. scabiei COX-1 gene isolated from Lamongan goats and Mojokerto rabbits were around 290 bp long. The sequences had more than 99% homology. The sequences of the COX-1 gene of S. scabiei from Lamongan goats and Mojokerto rabbits were relatively close to the sequence of the gene in S. scabiei obtained from various hosts according to National Center for Biotechnology Information data.

Clinical and molecular detection of Sarcoptes scabiei in the Iraqi camels

“…Interestingly, it is mentioned that the mange of camels can predominately be identified based on clinical signs (21). Therefore, these observations are consistent with the clinical signs of mange caused by S. scabies, where they agreed with other previous studies (21)(22)(23)(24). The molecular diagnosis (cPCR) based on COX1 shows that 29.41% of the examined camels were infested with S. scabiei.…”

Characterization of mitochondrial COX-1 gene of Sarcoptes scabiei from rabbits in East Java, Indonesia

“…Research by Lastuti et al [21] demonstrated that the partial CDS of mitochondrial COX-1 gene of S. scabiei from Lamongan goats was very similar to Mojokerto rabbits, with a homology identity of 99%. That previous study confirmed that out of 290 bp in Lamongan scabies-infected goats, there was one base pair different.…”

Sarcoptic Mange is an Emerging Threat to Biodiversity in the Qinling Mountains in China

“…The ITS2 region and cox 1 gene are widely used in the molecular diagnosis of sarcoptic mange, and ITS2 sequencing is more suitable for phylogenetic studies of astigmatid mites than the characterization of different species based on geographical distribution (Alasaad et al, 2009;Oleaga et al, 2012). However, the cox 1 gene is also suitable for the phylogenetic analysis of S. scabiei (Gomez-Puerta et al, 2022;Lastuti et al, 2019;Peltier et al, 2017b). S. scabiei is distributed among three genetically distinct clades; human-origin S. scabiei from Panama and Australia comprise 2 distinct clades, respectively, and rabbit-originS.…”