Sequence analysis of the maize mitochondrial 26 S rRNA gene and flanking regions

The Genetics, Pathology, and Molecular Biology of T-Cytoplasm Male Sterility in Maize

“…11 ; Dewey et al, 1986;Wise et al, 1987a). Interestingly, the insertion of the tandem TCTCA repeat in the T-urfl3 coding region in the T-4 mutant creates 86 nucleotides of perfect identity with the 3' flanking region of the rrn26 sequence (Dale et al, 1984), consistent with the hypothesis that T-4 also arose by homologous recombination or gene conversion with similar sequences in the mitochondrial genome (Wise et al, 1987a;Pring et al, 1988).…”

Mutation to male fertility and toxin insensitivity in Texas (T)-cytoplasm maize is associated with a frameshift in a mitochondrial open reading frame

“…The amino acid sequence derived from T ORF13 predicts a polypeptide of 12.9 kDa. Computer retrieval of matching sequence data on the Intelligenetics program revealed homology with 26S ribosomal DNA (25) and with a region on the 3' side of the 26S rDNA (24).…”

“…These changes in the T-4 mutant result in 86 bp of perfect homology with a region on the 3' side of 26S rDNA (23,25), including a tandem 5-bp repeat. This suggests that the altered sequences in the T-4 mutant arose by homologous recombination or gene conversion with the region on the 3' side of 26S rDNA.…”

Structure and function of ribosomal RNA

“…The most extreme example is the ribosome of Crithidia fasciculata, whose large subunit contains no less than four extra small RNA species in addition to 5S and 5.8S RNA (Schnare et al, 1983), although it has yet to be established whether all of these have 23S RNA counterparts. In contrast to this type of extra processing event, it has recently been proposed that some fungal mitochondrial ribosomes (which, as noted above, lack 5S RNA) may have actually incorporated a 5S-like sequence into their large subunit RNA molecules Dron et al (1982) Brosius et al (1978, Carbon et al (1978 Carbon et al (1981) Kop et al (1984a) Iwami et al (1984) 12S Eperon et al (1983) 16S Van Etten et al (1980) 16S Saccone et al (1981) 16S Eperon et al (1980) 16S Anderson et al (1982) 20Sb Seilhammer & Cummings (1981) 20Sb Seilhammer et al (1984b) 21S Sor & Fukuhara (1983) 23S Kochel & Kuntzel (1982) 26S Dale et al (1984) 23SC Edwards & Kossel (1981) 23SC Takaiwa & Sugiura (1982) 23S Brosius et al (1980), Branlant et al (1981) 23S Kumano et al (1983, Douglas & Doolittle (1984) 23S Kop et al (1984b) 16S Gupta et al (1983) 16S Leffers & Garrett (1984) McCarroll et al (1983) Takaiwa et al (1984) Messing et al (1984) Rubtsov et al (1980 Hadjiolov et al (1984), Chan et al (1983) There are a small number of modified nucleotides in ribosomal RNA. In E. coli, the 16S rRNA contains nine methylated bases (Carbon et al, 1979) and the 23S rRNA ten methylated bases and three pseudouridine residues .…”