2008
DOI: 10.1007/s11262-008-0213-5
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Sequence determination of a mildly virulent strain (CU-2) of Gallid herpesvirus type 2 using 454 pyrosequencing

Abstract: The complete DNA sequence of the mildly virulent Gallid herpesvirus type 2 strain CU-2 was determined and consists of 176,922 bp with an overall gene organization typical of class E herpesviruses. Phylogenetically, this strain partitions in its own branch between the virulent strains RB-1B, Md11, and Md5, and the vaccine strain CVI988. Overall, the genome of CU-2 is more similar to that of CVI988, with identically sized unique short regions of 11,651 bp. As in CVI988, an insertion of 177 bp was identified in t… Show more

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Cited by 43 publications
(35 citation statements)
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“…The overall coverage of 339 is similar to the coverage obtained in studies where the entire genome has been sequenced [50,51].…”
Section: Resultssupporting
confidence: 66%
“…The overall coverage of 339 is similar to the coverage obtained in studies where the entire genome has been sequenced [50,51].…”
Section: Resultssupporting
confidence: 66%
“…Accordingly, we will try to characterize the molecular basis associated with the enhanced pathogenicity and virulence of the TJ strain. For the larger size of herpesvirus genomes, conventional sequencing methods usually work less efficiently to rapidly get sequence data of novel virus strains (Spatz and Rue, 2008), and also the high G + C content of the genome, averaging 74%, makes reliable sequencing extremely difficult. For a long time, sequence determinations remained limited to fragments encompassing one or a few Single AA residues changes are written in standard format, including AA of the earlier isolates, its position, and the AA residue found in protein sequence of the TJ strain and/or recent isolates, e.g., S100P.…”
Section: Discussionmentioning
confidence: 99%
“…MDV causes Marek's disease in chickens with lymphomas (14). So far, the genome sequences of a total of 10 MDV strains have been analyzed (4,6,7,8,9,10,13,16). The GX0101 strain in this report was the first recombinant MDV field strain containing the reticuloendotheliosis virus (REV) long terminal repeat (LTR) insert (17), and it is a very virulent MDV with higher horizontal transmission ability (1,12).…”
mentioning
confidence: 97%