Sequence of RAD54, a Saccharomyces cerevisiae gene involved in recombination and repair

Emery, Herschell S.; Schild, David; Kellogg, David E.; Mortimer, Robert

doi:10.1016/0378-1119(91)90473-o

Cited by 90 publications

(41 citation statements)

References 13 publications

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“…Drosophila brm is required for activation of a set of homeotic genes (Tamkun et al 1992). ERCC6/CSBC, Rad54, Radl6, and Rad5 are involved in repair of DNA damage (Bang et al 1992;Johnson et al 1992;Toelstra et al 1992;Emery et al 1993). Drosophila lodestar is required for proper segregation of chromosomes (Girdham and Glover 1991).…”

Section: Function Of Snf2/swi2 Family Membersmentioning

confidence: 99%

“…ERCC6 (also known as CSBC) is a human gene involved in preferential repair of transcribed genes (Toelstra et al 1992). RAD5 (Johnson et al 1992), RAD16 (Bang et al 1992), and RAD54 (Emery et al 1993} are yeast genes involved in DNA repair. Drosophila lodestar encodes a function required for chromosome segregation (Girdham and Glover 1991).…”

Section: Adi Is Encoded By Mot1mentioning

confidence: 99%

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Mot1, a global repressor of RNA polymerase II transcription, inhibits TBP binding to DNA by an ATP-dependent mechanism.

Auble

Hansen²,

Mueller³

et al. 1994

Genes Dev.

289

328

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Section: Function Of Snf2/swi2 Family Membersmentioning

confidence: 99%

Section: Adi Is Encoded By Mot1mentioning

confidence: 99%

Mot1, a global repressor of RNA polymerase II transcription, inhibits TBP binding to DNA by an ATP-dependent mechanism.

Auble

Hansen²,

Mueller³

et al. 1994

Genes Dev.

289

328

View full text Add to dashboard Cite

show abstract

“…The RAD6 group (postreplication repair [PRR]) is required to overcome damages during replication and arrested replication forks. Interestingly, in all three DNA repair groups a member of the DNA helicase-like SNF2 gene family (Eisen et al, 1995;Flaus et al, 2006) is present, namely RAD16 (in RAD3 group; Bang et al, 1992), RAD54 (in RAD52 group; Emery et al, 1991), and RAD5 (in RAD6 group).…”

mentioning

confidence: 99%

A Homolog ofScRAD5Is Involved in DNA Repair and Homologous Recombination in Arabidopsis

et al. 2008

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Rad5 is the key component in the Rad5-dependent error-free branch of postreplication repair in yeast (Saccharomyces cerevisiae). Rad5 is a member of the Snf2 ATPase/helicase family, possessing as a characteristic feature, a RING-finger domain embedded in the Snf2-helicase domain and a HIRAN domain. Yeast mutants are sensitive to DNA-damaging agents and reveal differences in homologous recombination. By sequence comparisons we were able to identify two homologs (AtRAD5a and AtRAD5b) in the Arabidopsis thaliana genome, sharing about 30% identity and 45% similarity to yeast Rad5. AtRad5a and AtRad5b have the same kind of domain organization with a higher degree of similarity to each other than to ScRad5. Surprisingly, both genes differ in function: whereas two independent mutants of Atrad5a are hypersensitive to the cross-linking agents mitomycin C and cis-platin and to a lesser extent to the methylating agent, methyl methane sulfonate, the Atrad5b mutants did not exhibit any sensitivity to all DNA-damaging agents tested. An Atrad5a/Atrad5b double mutant resembles the sensitivity phenotype of the Atrad5a single mutants. Moreover, in contrast to Atrad5b, the two Atrad5a mutants are deficient in homologous recombination after treatment with the double-strand break-inducing agent bleomycin. Our results suggest that the RAD5-dependent error-free branch of postreplication repair is conserved between yeast and plants, and that AtRad5a might be functionally homologous to ScRad5.

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“…To ®nd a gene homologous to the yeast recombination gene RAD54 (Emery et al, 1991), an expressed sequence-tagged (EST) DNA fragment (GenBank accession number N30465) was used to screen a human testis cDNA library. We isolated a cDNA fragment predicted to encode a protein of 910 amino acids ( Figure 1).…”

mentioning

confidence: 99%

Mutations of a novel human RAD54 homologue, RAD54B, in primary cancer

et al. 1999

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Association of breast tumor susceptibility gene products BRCA1 and BRCA2 with the RAD51 recombination protein suggested that cancer could arise through defects in recombination. The identi®cation of NBS1, responsible for Nijmegen breakage syndrome, from the MRE11/RAD50 recombination protein complex also supports this hypothesis. However, our mutation analysis revealed that known members of the RAD52 epistasis group are rarely mutated in human primary cancer. Here we describe the isolation of a novel member of the SNF2 superfamily, characterized with sequence motifs similar to those in DNA and RNA helicases. The gene, designated RAD54B, is signi®cantly homologous to the RAD54 recombination gene. The expression of RAD54B was high in testis and spleen, which are active in meiotic and mitotic recombination. These ®ndings suggest that RAD54B may play an active role in recombination processes in concert with other members of the RAD52 epistasis group. RAD54B maps to human chromosome 8q21.3-q22 in a region associated with cancer-related chromosomal abnormalities. Homozygous mutations at highly conserved positions of RAD54B were observed in human primary lymphoma and colon cancer. These ®ndings suggest that some cancers arise through alterations of the RAD54B function.

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Sequence of RAD54, a Saccharomyces cerevisiae gene involved in recombination and repair

Cited by 90 publications

References 13 publications

Mot1, a global repressor of RNA polymerase II transcription, inhibits TBP binding to DNA by an ATP-dependent mechanism.

Mot1, a global repressor of RNA polymerase II transcription, inhibits TBP binding to DNA by an ATP-dependent mechanism.

A Homolog ofScRAD5Is Involved in DNA Repair and Homologous Recombination in Arabidopsis

Mutations of a novel human RAD54 homologue, RAD54B, in primary cancer

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