Sequence-specific electrochemical biosensing of M. tuberculosis DNA

Wang, Joseph; Rivas, Gustavo A.; Cai, Xiaohua; Dontha, Narasaiah; Shiraishi, Haruki; Luo, Den-Bai; Valera, Florenda S.

doi:10.1016/s0003-2670(96)00395-9

Cited by 97 publications

(35 citation statements)

References 18 publications

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“…In another study, using the same indicator with carbon paste, Mycobacterium tuberculosis bacteria with detection limit as low as 3.4 nM was reported [72,74]. Ferrocenylnapthalene diimide, nanoparticles and methylene blue are other indicators being used for the labelled approaches to achieve lower detection limits [75,76]. An enzyme indicator with magnetic separator was also used to avoid the selectivity problems [77].…”

Section: Biosensingmentioning

confidence: 99%

Synthesis and Biomedical Applications of Graphene: Present and Future Trends

Kumar¹,

Lee²

2013

Advances in Graphene Science

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Section: Biosensingmentioning

confidence: 99%

Synthesis and Biomedical Applications of Graphene: Present and Future Trends

Kumar¹,

Lee²

2013

Advances in Graphene Science

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“…Wang and coworkers developed a sensor that relies on the modification of the carbon-paste transducer oligonucleotide probe and their hybridization to complementary strands from the M. tuberculosis DNA (Wang et al, 1997). Chronopotentiometry was employed as transducer and the sensor allowed detection down to nanograms per milliliter of M. tuberculosis DNA.…”

Section: Nano-fabricated Devicesmentioning

confidence: 99%

Nanodiagnostics for Tuberculosis

Veigas¹,

Dória²,

Baptista³

2012

Understanding Tuberculosis - Global Experiences and Innovative Approaches to the Diagnosis

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“…This phenomenon is possible because of the biochemical property of base-pairing, which allows fragments of known sequences to find complementary matching sequences in an unknown DNA sample [1]. DNA hybridization biosensors can be employed for determining early and precise diagnosis of infectious agents in various environments [2,3] and these devices can be exploited for monitoring sequence-specific hybridization events directly [4,5] or by DNA intercalators (metal coordination complexes, antibiotics, etc.) which form complexes with the nitrogenous bases of DNA [6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Detection of short oligonucleotide sequences using an electrochemical DNA hybridization biosensor

Girousi

Kinigopoulou

2010

Open Chemistry

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An electrochemical DNA hybridization biosensor was developed for the detection of DNA hybridization using MDB and proflavine as electrochemical labels. The biosensor was based on the interaction of 7-dimethyl-amino-1,2-benzophenoxazi-nium Meldola's Blue (MDB) and proflavine with double stranded DNA (dsDNA) The electrochemical behaviour of MDB and proflavine as well as its interaction with double stranded (dsDNA) were investigated by cyclic (CV) and square wave voltammetry (SWV) and screen printed electrodes (ScPE). Furthermore, DNA-hybridization biosensors were developed for the detection of hybridization between oligonucleotides, which was detected by studying changes in the voltammetric peaks of MDB (reduction peak at -0.251 V) and proflavine (reduction peak at 0.075 V). MDB and proflavine were found to intercalate between the base pairs of dsDNA and oligonucleotides. Several factors affecting the dsDNA or oligonucleotides immobilization, hybridization and indicator preconcentration and interaction time, were investigated. As a result of the interaction of MDB with dsDNA and hybridized oligonucleotides, the voltammetric signals of MDB increased. Furthermore, guanine's oxidation peak (at 0.901 V) was decreased as MDB's concentration was increased. As a result of the interaction of proflavine with dsDNA and hybridized oligonucleotides, the voltammetric signals of proflavine decreased. These results were similar for carbon paste and screen printed electrodes. A comparison of the performance between CPE and ScPE was done. Our results showed that lower concentrations of MDB and proflavine were detected using screen printed electrodes. Moreover, reproducibility was better using screen printed electrodes and the detection was faster (regarding the experimental steps), but they are more cost effective.

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Sequence-specific electrochemical biosensing of M. tuberculosis DNA

Cited by 97 publications

References 18 publications

Synthesis and Biomedical Applications of Graphene: Present and Future Trends

Synthesis and Biomedical Applications of Graphene: Present and Future Trends

Nanodiagnostics for Tuberculosis

Detection of short oligonucleotide sequences using an electrochemical DNA hybridization biosensor

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