Sequentially-crosslinked biomimetic bioactive glass/gelatin methacryloyl composites hydrogels for bone regeneration

Zheng, Jiafu; Zhao, Fang; Wen, Zheng; Mo, Yunfei; Zeng, Lei; Li, Xian; Chen, Xiaofeng

doi:10.1016/j.msec.2018.03.029

Cited by 81 publications

(70 citation statements)

References 56 publications

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“…A gradient yield of Ca 2+ and Pi was also noted for other types of CaP-containing scaffolds [ 35 ], accompanied by the formation of an alkaline microenvironment that determines the realization of the osteogenic potential of MSCs [ 36 ]. This potential can be realized through various mechanisms, including CaR activity, the activation of osteoblastic differentiation and the formation of mineralization nodules in the ECM [ 6 ].…”

Section: Resultsmentioning

confidence: 97%

“…ALPL gene expression occurs during the early stages of osteogenesis [ 12 ]. ALP activity is necessary for subsequent mineralization of the ECM in the alkaline microenvironment [ 36 ] and appears within 7–14 days after contact with osteogenic scaffolds [ 35 ]. Ca 2+ ions formed during degradation of biomaterials are inducers of ALP expression [ 35 ] and the differentiation of MSCs into osteoblasts is triggered through the expression of calcium-binding proteins [ 39 ].…”

Section: Resultsmentioning

confidence: 99%

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Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating

Litvinova

Yurova

Шуплецова

et al. 2020

IJMS

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The manufacture of biomaterial surfaces with desired physical and chemical properties that can directly induce osteogenic differentiation without the need for biochemical additives is an excellent strategy for controlling the behavior of mesenchymal stem cells (MSCs) in vivo. We studied the cellular and molecular reactions of MSCs to samples with a double-sided calcium phosphate (CaP) coating and an average roughness index (Ra) of 2.4–4.6 µm. The study aimed to evaluate the effect of a three-dimensional matrix on the relative mRNA expression levels of genes associated with the differentiation and maturation of MSCs toward osteogenesis (RUNX2, BMP2, BMP6, BGLAP, and ALPL) under conditions of distant interaction in vitro. Correlations were revealed between the mRNA expression of some osteogenic and cytokine/chemokine genes and the secretion of cytokines and chemokines that may potentiate the differentiation of cells into osteoblasts, which indicates the formation of humoral components of the extracellular matrix and the creation of conditions supporting the establishment of hematopoietic niches.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating

Litvinova

Yurova

Шуплецова

et al. 2020

IJMS

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show abstract

“…The swelling behavior of hydrogels is important for the transportation of nutrients and wastes. [ 43 ] As shown in Figure A, swelling equilibrium was reached within 21 h for all hydrogels, which is attributed to their highly interconnected porous structure. [ 44 ] However, the SR of Gel‐OG hydrogel is higher than that of hybrid hydrogels.…”

Section: Resultsmentioning

confidence: 98%

A Biomimetic Hybrid Hydrogel Based on the Interactions between Amino Hydroxyapatite and Gelatin/Gellan Gum

Wang

et al. 2020

Macro Materials & Eng

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In this work, a gelatin (Gel)‐oxidized gellan gum (OG)/amino hydroxyapatite (mHap) hybrid hydrogel with Schiff base linkages is reported. The mHap is obtained by modifying hydroxyapatite with tetraethyl orthosilicate and 3‐aminopropyl‐triethoxysilane. The effects of different mHap contents on the structure, morphology, and properties of hydrogels are particularly investigated. Scanning electron microscopy coupled with energy dispersion spectroscopy reveals that mHap of around 100 nm is uniformly distributed inside the hydrogel with interconnected porous structures. Notably, the hydrogel with 1 wt% mHap possesses the highest compressive stress (2.01 ± 0.10 MPa) at 90% strain, as well as the lowest equilibrium swelling ratio (97% ± 5%) and degradation rate than other hydrogels. Besides, an ultra‐high compressive stress equivalent to 91% of the initial stress can be obtained by this hydrogel after 50 loading‐unloading cycles (85% strain). Meanwhile, after being swollen, this improved hydrogel also exhibits better structural stability than Gel‐OG hydrogel. The in vitro 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay further shows that all hydrogels are nontoxic against mouse fibroblasts. This work provides a biomimetic strategy to construct the organic/inorganic hydrogels with excellent interactions, elasticity, reversibility, and biocompatibility, which is of great importance for the practical applications in cartilage tissue engineering.

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“…However, when the bioactive glass content increased, the pore size also increased for the incubated and UV irradiated hydrogels. Bioactive glass proved to enhance the stability and bioactivity of the hydrogels and ensure rBMSCs attachment, proliferation, and differentiation (Zheng et al, 2018).…”

Section: New Approaches For Gelatin-based Biomaterialsmentioning

confidence: 99%

A review of chitosan-, alginate-, and gelatin-based biocomposites for bone tissue engineering

2018

Biomater Tissue Eng Bull

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Bone diseases and injuries have a major impact on the quality of life. Classical treatments for bone repair/regeneration/replacement have various disadvantages. Bone tissue engineering (BTE) received a great attention in the last years. Natural polymers are intensively studied in this field due to their properties (biocompatibility, biodegradability, abundance in nature, high processability). Unfortunately, their mechanical properties are poor, which is why synthetic polymers or ceramics are added in order to provide the optimal compressive, elastic or fatigue strength. Moreover, growth factors, vitamins, or antimicrobial substances are also added to enhance the cell behavior (attachment, proliferation, and differentiation). In this review, new scientific results regarding potential applications of chitosan-, alginate-, and gelatin based biocomposites in BTE will be provided, along with their in vitro and/or in vivo tests.

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Sequentially-crosslinked biomimetic bioactive glass/gelatin methacryloyl composites hydrogels for bone regeneration

Cited by 81 publications

References 56 publications

Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating

Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating

A Biomimetic Hybrid Hydrogel Based on the Interactions between Amino Hydroxyapatite and Gelatin/Gellan Gum

A review of chitosan-, alginate-, and gelatin-based biocomposites for bone tissue engineering

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