Serodiagnosis of Mycobacterium avium infections in pigs

Evaluation of a MPB70-ELISA to differentiate Mycobacterium bovis from M. avium-sensitized swine

“…ELISA was performed on non-infected animals from a free herd, which had most probably no previous contact with either virulent or environmental mycobacteria. Additionally, it is well-known that ELISAs performed with a single purified protein lead to lower ODs (Marassi et al 2011) and animals were sensitized only once, which reduces the intensity of the reaction (Wisselink et al 2010). Additionally, it has been reported that age can influence ELISA results in swine (Garcia-Bocanegra et al 2012) and in the present study we have only tested young animals.…”

“…mycobacteria from the tuberculosis complex, as Mycobacterium bovis, agent of bovine tuberculosis (TB) (Wisselink et al 2010). Conversely, other mycobacteria, such as members of the Mycobacterium avium complex (MAC), lead to a granulomatous lymphadenitis (GL)in head and mesenteric lymph nodes that, despite its economic importance due to the condemnation of carcasses (Faldyna et al 2012), present a lower public health risk, often restricted to immunocompromised people.…”

“…Although serological assays have been developed with promising results (Boadella et al 2011, Wisselink et al 2010, as well as an Interferon-gamma release assay (Faldyna et al 2012), they are mainly focused on the diagnosis of lymphadenitis, which is determined by MAC. To our knowledge, there are no studies addressing the diagnosis of M. bovis infections in swine and/or designed to discriminate between infections determined by M. bovis or MAC.…”

Non-Tuberculous Mycobacteria in Uganda: A Problem or Not?

“…This is largely due to the fact that majority of people in Uganda cannot afford these extra costs to arrive at a definitive diagnosis. Fortunately, quickcheaper, specific and sensitive diagnostic tools have been developed for example the ESAT-6 polymerase chain reaction primers which has been tailor-made to definitively diagnose M. avium, Serotyping methods using serotype specific sera for Mycobacterium interacellulare scrofulaceum (MAIS complex) and M. avium usually associated with AIDS patients can also be used in small and medium scale laboratories for definitive diagnosis (Kiehn et al, 1985;Singh et al, 2007;Wisselink et al 2010). NTM are generally resistant to the standard therapy Isoniazid, Rifampicin and Clarithromycin (Bum-Joon et al 2004).…”

Prevalence of Mycobacterium avium in Slaughter Pigs Based on Serological Monitoring Results and Bacteriological Validation

“…However, the incision of the lymph nodes is characterized by relatively high false positive and false negative results for MA [12,13]. In addition, it can cause cross-contamination with other food safety hazards, e.g., salmonella [14,15].…”