Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan

Wu, Ho-Sheng; Chiu, Shu-Chun; Tseng, Tsan-Chang; Lin, Szu-Fong; Lin, Jer-Huei; Hsu, Yu-Fen; Wang, Mei-Ching; Lin, Tsuey-Li; Yang, Wei-Shun; Ferng, Tian-Lin; Huang, Kuan-Gen; Hsu, Li-Chuan; Lee, Li-Li; Yang, Pan‐Chyr; Chen, Hour-Young; Su, Shun-Pi; Yang, Shih-Yan; Lin, Tao; Su, Ih‐Jen

doi:10.3201/eid1002.030731

Cited by 101 publications

(142 citation statements)

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“…Numerous procedures based on nucleic acid amplification have been reported (84,85). These include RT-PCR and nucleic acid sequencebased amplification (NASBA).…”

Section: Detection Of Virus/viral Rna Although Cells Such As Vero Canmentioning

confidence: 99%

Severe Acute Respiratory Syndrome (SARS): A Year in Review

et al. 2005

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Severe acute respiratory syndrome (SARS) emerged from China as an untreatable and rapidly spreading respiratory illness of unknown etiology. Following point source exposure in February 2003, more than a dozen guests infected at a Hong Kong hotel seeded multi-country outbreaks that persisted through the spring of 2003. The World Health Organization responded by invoking traditional public health measures and advanced technologies to control the illness and contain the cause. A novel coronavirus was implicated and its entire genome was sequenced by mid-April 2003. The urgency of responding to this threat focused scientific endeavor and stimulated global collaboration. Through real-time application of accumulating knowledge, the world proved capable of arresting the first pandemic threat of the twenty-first century, despite early respiratory-borne spread and global susceptibility. This review synthesizes lessons learned from this remarkable achievement. These lessons can be applied to re-emergence of SARS or to the next pandemic threat to arise.

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“…Numerous procedures based on nucleic acid amplification have been reported (84,85). These include RT-PCR and nucleic acid sequencebased amplification (NASBA).…”

Section: Detection Of Virus/viral Rna Although Cells Such As Vero Canmentioning

confidence: 99%

Severe Acute Respiratory Syndrome (SARS): A Year in Review

et al. 2005

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“…SARS originated from Southern China and then spread to many countries and regions, including Taiwan [Chan et al, 2004]. The latter experienced a SARS outbreak from March to June of 2003 and suffered from 680 probable cases of SARS with 81 confirmed deaths [Wu et al, 2004]. In our hospital, a 23-year-old patient recovered from SARS and was ready for discharge on the 17th day after the onset of the disease.…”

Section: Introductionmentioning

confidence: 98%

False positive antibody results against human T‐cell lymphotropic virus in patients with severe acute respiratory syndrome

Tsao

Chen

Huang

et al. 2005

Journal of Medical Virology

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Taiwan suffered from the outbreak of severe acute respiratory syndrome (SARS) in 2003. Our laboratory performed a series of virology and serology tests for SARS patients admitted to our hospital. Cross-reactivity was found when testing for antibody against human T-cell lymphotropic virus (HTLV) in one patient with SARS. Therefore, antibodies against HTLV were examined in paired-sera from 26 SARS patients. ELISA and a neutralization test were used to measure anti-SARS antibodies. Seroconversion for antibody against SARS-CoV was observed in all patients. Surprisingly, with the use of ELISA for HTLV, sera for 13 patients were positive for HTLV (50%), and seroconversion for HTLV was also observed in 10 patients (38.5%). Western blot for HTLV on those 26 paired-sera from 13 HTLV-positive patients displayed 5 positive results for HTLV-I, 7 positive results for HTLV-II, 1 positive result for both HTLV-I and II, 9 negative results for either HTLV-I or HTLV-II, and 4 "indeterminate" results. The findings that antibody to HTLV can be detected in blood samples collected from SARS patients provide important information for safe handling of blood products. Without such knowledge, blood products can be discarded mistakenly even though they contain anti-SARS-CoV antibodies that may be potentially valuable for SARS therapy.

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“…Several serological studies with SARS patient sera have been reported and these show varying sensitivities and specificities. 5,6,8 The reference serological method is the neutralisation test and this was compared to enzyme linked immunosorbent assay (ELISA), immunofluorescence assays (IFA) and the immunochromatic test (ICT). Antibody determination using IFA or ELISA was the most reliable method for identifying infections with SARSCoV.…”

Section: Serological Testsmentioning

confidence: 99%