Serologic Evidence of Flavivirus Infection in Bats in the Yucatan Peninsula of Mexico

Machaín-Williams, Carlos; López-Uribe, Mildred P.; Talavera-Aguilar, Lourdes G.; Carrillo-Navarrete, Jaquelin; Vera-Escalante, Luis; Puerto, Fernando I.; Ulloa, Armando; Farfán-Ale, José A.; Garcia-Rejon, Julián E.; Blitvich, Bradley J.; Loroño-Pino, María Alba

doi:10.7589/2012-12-318

Cited by 39 publications

(34 citation statements)

References 17 publications

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“…Serologic evidence for DENV infection in several species of chiroptera collected in Ecuador, Costa Rica, and Mexico has also been reported. [12][13][14] In addition, reverse transcription-polymerase chain reaction (RT-PCR) testing of spleen or heart tissue samples from bats captured in dengue-endemic areas in Mexico, detected the presence of DENV-2 in frugivorous (Artibeus jamaicensis, Artibeus lituratus, Glossophaga soricina) and insectivorous (Myotis nigricans) bats. 13,15 In an elegant study carried out in the French Guiana, RNA corresponding to the 4 DENV serotypes was detected in tissue samples collected from several species of wild mammals.…”

Section: Introductionmentioning

confidence: 99%

Experimental Inoculation of Artibeus jamaicensis Bats with Dengue Virus Serotypes 1 or 4 Showed No Evidence of Sustained Replication

Cabrera-Romo¹,

Recio-Tótoro²,

Alcalá³

et al. 2014

The American Society of Tropical Medicine and Hygiene

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Abstract. Dengue is the most important mosquito-borne viral disease to humans. Bats are potential reservoirs for flaviviruses, including dengue virus (DENV). In this work, Artibeus jamaicensis bats were inoculated with two serotypes of DENV using different routes. For experimental inoculations (EI) 1 and 2, bats were inoculated subcutaneously or intraperitoneally with DENV-4; for EI-3 bats were inoculated intraperitoneally with DENV-1. Mock inoculated bats were kept as controls. In EI-4, bats were bitten by Aedes aegypti mosquitoes infected with DENV-1 or 4. Reverse transcription-polymerase chain reaction assays in plasma and spleen tissue collected from Day 1 to Days 9-17 after inoculation failed to reveal the presence of viral RNA in any of the samples. No evidence of circulating NS1 or specific anti-DENV IgG was detected in the plasma of the inoculated bats. These results indicate that A. jamaicensis bats are incapable of sustaining dengue virus replication and are unlikely to act as reservoirs for this virus.

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Section: Introductionmentioning

confidence: 99%

Experimental Inoculation of Artibeus jamaicensis Bats with Dengue Virus Serotypes 1 or 4 Showed No Evidence of Sustained Replication

Cabrera-Romo¹,

Recio-Tótoro²,

Alcalá³

et al. 2014

The American Society of Tropical Medicine and Hygiene

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“…7 Qualitative detection of the NS1 antigen of DENVs in bat serum samples and plaque reduction neutralization tests has been reported in bats from southeastern Mexico from disturbed sites near human settlements in Campeche (S. Cabrera-Romo and others, unpublished data) and Mérida,17 which is the largest city in the Yucatan Peninsula.…”

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confidence: 99%

“…16 Molecular evidence of DENV-1, -2, and -3 in bats from French Guiana was reported, including a phylogenetic sequence from a Carollia perspicillata bat consistent with DENV-1. 7 Qualitative detection of the NS1 antigen of DENVs in bat serum samples and plaque reduction neutralization tests has been reported in bats from southeastern Mexico from disturbed sites near human settlements in Campeche (S. Cabrera-Romo and others, unpublished data) and Mérida, 17 which is the largest city in the Yucatan Peninsula.…”

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confidence: 99%

“…7,[15][16][17] Unfortunately, because of sample degradation, we could not amplify a different region of the viral genome for phylogenetic analysis from the positive samples. Although DENV infection was serologically recognized in 26 bats of the same three species (A. jamaicensis, A. lituratus, and G. soricina) from Merida City, 17 this detection is the first detection of the DENV-2 genome by molecular diagnostic methods in bats from southeastern Mexico. DENV-1, -2, and -3 have been reported in bats from French Guiana by RT-PCR, where DENV was found in A. planirostris and C. perspicillata.…”

mentioning

confidence: 99%

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Dengue Virus in Bats from Southeastern Mexico

Sotomayor-Bonilla¹,

Chaves²,

Rico-Chávez³

et al. 2014

The American Society of Tropical Medicine and Hygiene

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Abstract. To identify the relationship between landscape use and dengue virus (DENV) occurrence in bats, we investigated the presence of DENV from anthropogenically changed and unaltered landscapes in two Biosphere Reserves: Calakmul (Campeche) and Montes Azules (Chiapas) in southern Mexico. Spleen samples of 146 bats, belonging to 16 species, were tested for four DENV serotypes with standard reverse transcriptase polymerase chain reaction (RT-PCR) protocols. Six bats (4.1%) tested positive for DENV-2: four bats in Calakmul (two Glossophaga soricina, one Artibeus jamaicensis, and one A. lituratus) and two bats in Montes Azules (both A. lituratus). No effect of anthropogenic disturbance on the occurrence of DENV was detected; however, all three RT-PCR-positive bat species are considered abundant species in the Neotropics and well-adapted to disturbed habitats. To our knowledge, this study is the first study conducted in southeastern Mexico to identify DENV-2 in bats by a widely accepted RT-PCR protocol. The role that bats play on DENV's ecology remains undetermined.Dengue fever is an important public health concern in the tropics, 1-4 and ecological and epidemiological studies are needed to assess the role of bats and other mammals in a possible sylvatic maintenance cycle.5 Dengue viruses (DENVs) comprise four antigenically distinct but genetically related serotypes of the Flavivirus genus (Flaviviridae family). 1DENVs are positive-sense single-stranded RNA viruses that cause one of the most common infectious diseases in humans in tropical regions.2 Their transmission includes an urban endemic/epidemic cycle between Aedes aegypti mosquitoes and humans as the reservoir host and a sylvatic enzootic cycle between non-human primates and arboreal mosquitoes of the genus Aedes.3 The urban cycle is well-documented in the Neotropics, with four serotypes reported in urban areas, 1-4 whereas the sylvatic cycle has been shown in West Africa and peninsular Malaysia.5 Thus far, the sylvatic cycle has not been described in the Neotropics. However, in Bolivia, DENV seroconversions among the indigenous Ayoreo people were found in a remote area where Ae. aegypti, the primary vector, was absent. 6 This finding suggests a possible sylvatic cycle involving a different mosquito species or cross-reaction with antibodies to another flavivirus. In French Guiana, all four DENV serotypes have been identified by molecular methods in 92 wild mammals (bats, rodents, and marsupials) in all settings investigated: periurban, rural, and sparsely populated areas.7 This finding suggests that primarily urban DENV strains could infect wildlife in non-urban forested areas. 7 The role of wildlife in DENV transmission remains unknown.Bats are important reservoirs of many viruses, such as rabies viruses, Nipah viruses, and coronaviruses. [8][9][10][11][12] Flaviviridae are the second most frequently reported viral family in the order Chiroptera (13% frequency; second only to rhabdoviruses) 9 ; however, their role in the dynamics of DENVs remains poorly ...

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“…The antibody-positive bats belonged to three species: the Pallas's long-tongued bat (Glossophaga soricina) (33%), Jamaican fruit bat (Artibeus jamaicensis) (24%), and great fruit-eating bat (Artibeus literatus) (9%). 37 On the Caribbean island of Trinidad, Thompson and others 38 found antibodies to Tamana bat virus (TABV) and RBV in 47 (15.3%) and three (1.0%) bats, respectively, using hemagglutination inhibition assay (HAI). Their results also suggested the presence of antibodies to an undetermined flavivirus(es) in eight (2.6%) bats.…”

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confidence: 99%

Flavivirus Infections of Bats: Potential Role in Zika Virus Ecology

Kading

Schountz

2016

The American Society of Tropical Medicine and Hygiene

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Abstract. Understanding the vector and nonhuman vertebrate species contributing to Zika virus (ZIKAV) transmission is critical to understanding the ecology of this emerging arbovirus and its potential to establish in new geographic areas. This minireview summarizes what is known regarding the association of bats with flaviviruses (Flaviviridae: Flavivirus) with a particular emphasis on the potential role of bats in the sylvatic transmission of ZIKAV. Key research directions that remain to be addressed are also discussed.

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Serologic Evidence of Flavivirus Infection in Bats in the Yucatan Peninsula of Mexico

Cited by 39 publications

References 17 publications

Experimental Inoculation of Artibeus jamaicensis Bats with Dengue Virus Serotypes 1 or 4 Showed No Evidence of Sustained Replication

Experimental Inoculation of Artibeus jamaicensis Bats with Dengue Virus Serotypes 1 or 4 Showed No Evidence of Sustained Replication

Dengue Virus in Bats from Southeastern Mexico

Flavivirus Infections of Bats: Potential Role in Zika Virus Ecology

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