Serologic Reports of H3N2 Canine Influenza Virus Infection in Dogs in Northeast China

Zhang, Yong-Biao; Chen, Jidang; Xie, Jiexiong; Zhu, Wanjun; Wei, Chunya; Tan, Likai; Cao, Nan; Chen, Ye; Zhang, Minze; Zhang, Guihong; Li, Shoujun

doi:10.1292/jvms.13-0022

Cited by 14 publications

(14 citation statements)

References 9 publications

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“…The continued interspecies transmission of IAVs to humans and other mammals is a constant threat (4,(39)(40)(41). As dogs are popular companion animals and can support the replication of multiple IAV subtypes such as H3N8, H3N1, H3N2, H5N1, H5N2, and H1N1 (10,11,(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53), they could act as intermediate hosts for IAV reassortment or human exposure (7). Various human IAVs, such as PR8, A/Udorn/307/72 H3N2, and pH1N1, replicate in the respiratory tract of the dog, and viable reassortant viruses between H3N8 CIV and pH1N1 IAV have been reported (54).…”

Section: Discussionmentioning

confidence: 99%

The K186E Amino Acid Substitution in the Canine Influenza Virus H3N8 NS1 Protein Restores Its Ability To Inhibit Host Gene Expression

Nogales

Chauché

DeDiego

et al. 2017

J Virol

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Canine influenza viruses (CIVs) are the causative agents of canine influenza, a contagious respiratory disease in dogs, and include the equine-origin H3N8 and the avian-origin H3N2 viruses. Influenza A virus (IAV) nonstructural protein 1 (NS1) is a virulence factor essential for counteracting the innate immune response. Here, we evaluated the ability of H3N8 CIV NS1 to inhibit host innate immune responses. We found that H3N8 CIV NS1 was able to efficiently counteract interferon (IFN) responses but was unable to block general gene expression in human or canine cells. Such ability was restored by a single amino acid substitution in position 186 (K186E) that resulted in NS1 binding to the 30-kDa subunit of the cleavage and polyadenylation specificity factor (CPSF30), a cellular protein involved in pre-mRNA processing. We also examined the frequency distribution of K186 and E186 among H3N8 CIVs and equine influenza viruses (EIVs), the ancestors of H3N8 CIV, and experimentally determined the impact of amino acid 186 in the ability of different CIV and EIV NS1s to inhibit general gene expression. In all cases, the presence of E186 was responsible for the control of host gene expression. In contrast, the NS1 protein of H3N2 CIV harbors E186 and blocks general gene expression in canine cells. Altogether, our results confirm previous studies on the strain-dependent ability of NS1 to block general gene expression. Moreover, the observed polymorphism on amino acid 186 between H3N8 and H3N2 CIVs might be the result of adaptive changes acquired during long-term circulation of avian-origin IAVs in mammals. Canine influenza is a respiratory disease of dogs caused by two CIV subtypes, the H3N8 and H3N2 viruses, of equine and avian origins, respectively. Influenza NS1 is the main viral factor responsible for the control of host innate immune responses, and changes in NS1 can play an important role in host adaptation. Here we assessed the ability of H3N8 CIV NS1 to inhibit host innate immune responses and gene expression. The H3N8 CIV NS1 did not block host gene expression, but this activity was restored by a single amino acid substitution (K186E), which was responsible for NS1 binding to the host factor CPSF30. In contrast, the H3N2 CIV NS1, which contains E186, blocks general gene expression. Our results suggest that the ability to block host gene expression is not required for influenza virus replication in mammals but might be important in the long-term adaptation of avian-origin influenza viruses to mammals.

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Section: Discussionmentioning

confidence: 99%

The K186E Amino Acid Substitution in the Canine Influenza Virus H3N8 NS1 Protein Restores Its Ability To Inhibit Host Gene Expression

Nogales

Chauché

DeDiego

et al. 2017

J Virol

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“…Canine influenza virus (CIV) infection was then reported in South Korea in 2008. Outbreaks of H3N2 CIV have been continuously reported in South Korea and China [11,12]. Outbreaks of H3N2 CIV have been continuously reported in South Korea and China [11,12].…”

Section: Introductionmentioning

confidence: 99%

H3N2 canine influenza virus with the matrix gene from the pandemic A/H1N1 virus: infection dynamics in dogs and ferrets

et al. 2014

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After an outbreak of pandemic influenza A/H1N1 (pH1N1) virus, we had previously reported the emergence of a recombinant canine influenza virus (CIV) between the pH1N1 virus and the classic H3N2 CIV. Our ongoing routine surveillance isolated another reassortant H3N2 CIV carrying the matrix gene of the pH1N1 virus from 2012. The infection dynamics of this H3N2 CIV variant (CIV/H3N2mv) were investigated in dogs and ferrets via experimental infection and transmission. The CIV/H3N2mv-infected dogs and ferrets produced typical symptoms of respiratory disease, virus shedding, seroconversion, and direct-contact transmissions. Although indirect exposure was not presented for ferrets, CIV/H3N2mv presented higher viral replication in MDCK cells and more efficient transmission was observed in ferrets compared to classic CIV H3N2. This study demonstrates the effect of reassortment of the M gene of pH1N1 in CIV H3N2.

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“…The influenza A virus has a wide host adaptation, ranging from avian to mammalian, but until recently has not been reported in dogs (3,12,30). Influenza virus infection in dogs was first detected in the United States in 2004, and genetic analyses of the virus showed an equinederived influenza virus (H3N8) (7,28). Initially, the canine influenza virus (CIV) in dogs was thought to occur because of contact with horses, but interspecies transmission of the CIV remained unclear (28).…”

Section: Praca Oryginalna Original Papermentioning

confidence: 99%

“…Influenza virus infection in dogs was first detected in the United States in 2004, and genetic analyses of the virus showed an equinederived influenza virus (H3N8) (7,28). Initially, the canine influenza virus (CIV) in dogs was thought to occur because of contact with horses, but interspecies transmission of the CIV remained unclear (28). Since the first detection of the disease in dogs, different subtypes of the influenza virus, including H5N1 (23,25), H3N2 (23), H5N2 (9), H1N1 (14), and H3N1 (24), have been reported.…”

Section: Praca Oryginalna Original Papermentioning

confidence: 99%

Serological evidence of canine influenza virus infection in shelter dogs in Turkey

Aydın

Kirbaş

Timurkan

et al. 2018

Medycyna Weterynaryjna

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Influenza virus infection is an important disease which occurs in humans and a variety of animals. Because of the wide host adaptation and segmented genome, there is always the possibility of mutations and interspecies transmission of the influenza virus. Our study is the first to draw attention to canine influenza infection in Turkey. For this purpose, 208 sera and swab samples were collected from dogs with respiratory and nonrespiratory signs in various seasons. Out of the 208 dogs, 94 (45.2%) were male and 114 (54.8%) were female; the average age was 4.7 years. A total of 208 sera samples were tested for the presence of canine influenza virusspecific antibodies by the indirect enzyme-linked immunosorbent assay. The seroprevalence of canine influenza virus infection was 11/208 (5.8%). With regard to seasonal distribution, the highest rate of seropositivity was detected in spring, and the lowest in summer. Molecular detection of the canine influenza virus from nasal swab samples was done by reverse transcriptase polymerase chain reaction using specific universal primers for the hemagglutinin gene. Influenza virus nucleic acid could not be detected by reverse transcriptase polymerase chain reaction. In this study, we revealed for the first time the existence of the canine influenza virus in Turkey. Although the seroprevalence was relatively low, it would be useful to investigate the canine influenza virus on a large scale and among dogs with infectious respiratory disease in the Turkish dog population..

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Serologic Reports of H3N2 Canine Influenza Virus Infection in Dogs in Northeast China

Cited by 14 publications

References 9 publications

The K186E Amino Acid Substitution in the Canine Influenza Virus H3N8 NS1 Protein Restores Its Ability To Inhibit Host Gene Expression

The K186E Amino Acid Substitution in the Canine Influenza Virus H3N8 NS1 Protein Restores Its Ability To Inhibit Host Gene Expression

H3N2 canine influenza virus with the matrix gene from the pandemic A/H1N1 virus: infection dynamics in dogs and ferrets

Serological evidence of canine influenza virus infection in shelter dogs in Turkey

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