Serological and Nucleic Acid Analyses for HIV and HTLV Infection on Archival Human Plasma Samples from Zaire

Dube, Dipak K.; Dube, Syamalima; Erensoy, Selda; Jones, Barbara; Bryz-Gornia, V; Spicer, Timothy; Love, Jayne L.; Saksena, Nitin K.; Lechat, M. F.; Shrager, Daniel I.; Dosik, Harvey; Glaser, Jordan B.; Levis, William R.; Blattner, William A.; Montagna, Richard A.; Blumberg, Baruch S.; Poiesz, Bernard J.

doi:10.1006/viro.1994.1354

Cited by 42 publications

(21 citation statements)

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“…Likewise, given the degree of conservation of the pol sequences in the FLK\BLV cell line and in PTLV-infected humans and cell lines tested to date Sherman et al, 1993), it is unlikely that the degree of BLV strain divergence observed in some cattle was the result of quasi-species formation following infection by one strain. Whereas we have found humans co-infected with both HTLV-I and HTLV-II (which diverge from each other by 40 %), we have yet to identify humans or non-human primates coinfected with multiple PTLV-I or PTLV-II strains (Ehrlich et al, 1989(Ehrlich et al, b, c, 1992Dube et al, 1993Dube et al, , 1994Dube et al, , 1995Saksena et al, 1994). These observations may reflect differences in immunological protection against BLV and PTLV infection in cattle vs primates, respectively, or they may be due to simultaneous inoculation of multiple BLV isolates into a particular cow via veterinary or animal husbandry techniques.…”

Section: S Dube and Others S Dube And Othersmentioning

confidence: 94%

Degenerate and specific PCR assays for the detection of bovine leukaemia virus and primate T cell leukaemia/lymphoma virus pol DNA and RNA: phylogenetic comparisons of amplified sequences from cattle and primates from around the world.

Dube

Bachman

Spicer

et al. 1997

Journal of General Virology

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Section: S Dube and Others S Dube And Othersmentioning

confidence: 94%

Degenerate and specific PCR assays for the detection of bovine leukaemia virus and primate T cell leukaemia/lymphoma virus pol DNA and RNA: phylogenetic comparisons of amplified sequences from cattle and primates from around the world.

Dube

Bachman

Spicer

et al. 1997

Journal of General Virology

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“…Fifty-three well characterized blood samples containing either HIV-1, HTLV-I, HTLV-II, or no retroviral DNA and four well characterized cell lines infected with HIV-2 [HIV-2͞ST (24), CBL-23 (28), HUT 78͞HIV-2͞D194 (29), and CEM-LAV2 (2)], were tested for the presence of each of the four retroviruses. These samples had been tested previously for the presence of retroviruses by using conventional serodiagnostic assays (30) and PCR amplification assays in which the target amplicons were detected with a solid-phase, colorimetric hybridization procedure (31). DNA was extracted from each blood sample by the quick lysis method (32), except for samples infected with HTLV-II, which were extracted organically (33), and 2-4 g of the extracted DNA (originating from approximately 500,000 cells) was used as template in each PCR assay.…”

Section: Methodsmentioning

confidence: 99%

Multiplex detection of four pathogenic retroviruses using molecular beacons

Vet

Majithia

Marras

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

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307

182

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We describe a multiplex nucleic acid assay that identifies and determines the abundance of four different pathogenic retroviruses (HIV-1, HIV-2, and human T-lymphotrophic virus types I and II). Retroviral DNA sequences are amplified in a single, sealed tube by simultaneous PCR assays, and the resulting amplicons are detected in real time by the hybridization of four differently colored, amplicon-specific molecular beacons. The color of the fluorescence generated in the course of amplification identifies which retroviruses are present, and the number of thermal cycles required for the intensity of each color to rise significantly above background provides an accurate measure of the number of copies of each retroviral sequence that were present originally in the sample. Fewer than 10 retroviral genomes can be detected. Moreover, 10 copies of a rare retrovirus can be detected in the presence of 100,000 copies of an abundant retrovirus. Ninety-six samples can be analyzed in 3 hr on a single plate, and the use of a closed-tube format eliminates crossover contamination. Utilizing previously well characterized clinical samples, we demonstrate that each of the pathogenic retroviruses can be identified correctly and no false positives occur. This assay enables the rapid and reliable screening of donated blood and transplantable tissues.

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“…This technique has high sensitivity and specificity with plasma (6). Using CFRS, we amplified a pol fragment in the three TSP/HAM patients and one asymptomatic carrier with a local IgA response who lacked detectable levels of systemic IgA.…”

Section: Discussionmentioning

confidence: 99%

Human T-Lymphotropic Virus Type I (HTLV-I)-Specific Antibodies and Cell-Free RNA in Crevicular Fluid-Rich Saliva from Patients with Tropical Spastic Paraparesis/HTLV-I-Associated Myelopathy

Soto-Ramírez

García-Vallejo²,

Renjifo³

et al. 1995

Viral Immunology

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Despite the likely role of mucosae in human T cell leukemia virus type I (HTLV-I) transmission, little is known about the mucosal immune response to HTLV-I. The present study evaluated the antibody response to HTLV-I in oral mucosa and the value of crevicular fluid rich saliva (CFRS) for diagnosing HTLV-I infection. CFRS and sera from patients with tropical spastic paraparesis/HTLV-I-associated myelopathy (TSP/HAM), asymptomatic carriers, and HTLV-I seronegative individuals from Tumaco, Colombia, were analyzed for HTLV-I specific IgG, IgA, and secretory IgA (sIgA). Detection of IgG in CFRS by enzyme-linked immunosorbent assay correlated with its presence in sera for TSP/HAM patients and asymptomatic carriers. IgA and sIgA were more frequently detected in CFRS and sera from TSP/HAM patients than in those from asymptomatic carriers. An HTLV-I pol fragment could be amplified from CFRS by reverse transcriptase-PCR in 3 TSP/HAM patients and one asymptomatic carrier, all of whom had an IgA response in CFRS but not in sera. The more frequent detection of IgA and sIgA in sera and CFRS of TSP/HAM patients suggests increased viral replication. Further, the association of viral RNA in CFRS with a local IgA response may signify rounds of viral replication in the oral cavity.

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Serological and Nucleic Acid Analyses for HIV and HTLV Infection on Archival Human Plasma Samples from Zaire

Cited by 42 publications

References 0 publications

Degenerate and specific PCR assays for the detection of bovine leukaemia virus and primate T cell leukaemia/lymphoma virus pol DNA and RNA: phylogenetic comparisons of amplified sequences from cattle and primates from around the world.

Degenerate and specific PCR assays for the detection of bovine leukaemia virus and primate T cell leukaemia/lymphoma virus pol DNA and RNA: phylogenetic comparisons of amplified sequences from cattle and primates from around the world.

Multiplex detection of four pathogenic retroviruses using molecular beacons

Human T-Lymphotropic Virus Type I (HTLV-I)-Specific Antibodies and Cell-Free RNA in Crevicular Fluid-Rich Saliva from Patients with Tropical Spastic Paraparesis/HTLV-I-Associated Myelopathy

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