Serological, Immunochemical and Immuoncytological Properties of Granulocyte Antibodies

Abstract

The immunochemical characteristics of granulocyte alio- and autoantibodies can easily be studied with indirect immunofluorescence techniques. The correlation of these properties with the serological behavior of the antibodies and the clinical condition in which they were found was studied. The investigations included the determination of immunoglobulin class, subclass, light-chain composition, as well as optimal temperature of activity. Complement fixation in vitro was also investigated. Striking differences were found between IgG and IgM antibodies in their serological properties, especially in the granulocytotoxicity and leukoagglutination tests at different temperatures. Differences in light-chain and IgG subclass compositions of the antibodies did not seem to play a role. In studying the distribution of granulocyte-specific antigens on normal granulocytes and granulocytes in all stages of development, it was found that, while NAl5 NA2 and ND! are present in the same quantity on the cells of an individual, the NBt antigen is unequally distributed, both over ripe granulocytes and granulocyte precursors.

Significance of the detection of antineutrophil antibodies in children with chronic neutropenia

“…15 Therefore, the combination of semiquantified GIIFT and agglutination test for detecting antineutrophil antibodies provide information for predicting the clinical course of children with chronic neutropenia. Further, a large prospective study is necessary to demonstrate that measurement of autoantibodies at the time of diagnosis will aid in observations of the natural history of this disorder.…”

“…3,4,7 Several methods are used in the detection of serum antineutrophil antibodies. [5][6][7][8][12][13][14][15][16][17] However, the specificity and sensitivity of these methods have not been well established. In this study, we have developed a method to semiquantify the strength of antineutrophil antibodies by flow cytometric analysis of the granulocyte indirect immunofluorescence test (GIIFT) by using same donor neutrophils.…”

Lack of NB1 GP (CD177/HNA-2a) gene transcription in NB1 GP- neutrophils from NB1 GP–expressing individuals and association of low expression with NB1 gene polymorphisms

“…[2][3][4] It shows the unique feature of being expressed on a neutrophil subpopulation ranging from 0% to 100%. 5 After identification of NB1 GP deficiency as the result of a splicing defect resulting in mRNA strands containing intron sequences with stop codons, we searched for the reason for its heterogeneous expression. 6 For this, we separated the 2 subpopulations by 2 different methods (laser-assisted microdissection and fluorescence-activated cell sorter), followed by qualitative and quantitative measurements of the mRNA content.…”

Autoimmune Neutropenia in Infancy Due to Anti-NA1 Antibody: Detection of Antibody with Immunofluorescence and Agglutination Test

“…Verheugt et al (4) suggested that strongly positive reactions in immunofluorescence were obtained not only with antisera containing agglutinins or granulocytotoxins, but also with some sera that did not react in either the agglutination or cytotoxicity test. In addition, immunofluorescence test appears to be a simple and sensitive method that allows us to study immunochemical characteristics of antibodies (19). In this study, antibodies detected with the agglutination test clearly discriminated between NAl + and NAl-neutrophils.…”