Serological Studies on the Major Histocompatibility Complex of New Inbred Strains of the Guinea Pig1

Abstract

New inbred strains of guinea pigs, JY 1, JY 2, JY 3, JY 6, JY 9 and JY 10 have been established in this Institute. Serologic studies of guinea pig leukocyte antigens (GPLA antigens) were carried out in order to examine their major histocompatibility complex (GPLA complex). Antisera specific for Ia antigens were raised by cross-immunization of NIH strain 2 (NIH 2) and NIH strain 13 (NIH 13) guinea pigs, well known inbred guinea pigs. The sera identified four distinct Ia specificities, which were designated as Ia.2a, Ia.2b, Ia.13a and Ia.13b. Six antigenic specificities different from the above Ia specificities were identified by sera obtained by appropriate immunization of the inbred guinea pigs and were designated as P.1, P.2, P.3, P.4, P.5 and P.6. Antigenic specificities of GPLA antigens recognized in inbred guinea pigs were: NIH 2 (Ia.2a, Ia.2b, P.1, P.2, P.3, P.4), NIH 13 (Ia.13a, Ia.13b, P.1, P.2, P.3, P.4), JY 1 (Ia.13a, Ia.13b, P.5), JY 2 (Ia.2b, Ia.13b, P.3, P.4, P.6), JY 3 (Ia.13a, Ia.13b, P.2, P.4, P.5), JY 6 (Ia.2b, Ia.13b, P.3, P.6), JY 9 (Ia.13a, Ia.13b, P.4, P.5), JY 10 (Ia.13a, Ia.13b, P.2, P.3, P.4, P.6). The correspondence of these specificities to those already reported was discussed and the P.2 or P.4 was considered to be an additional specificity of GPLA antigens that have not been reported yet. As the new inbred strains of guinea pigs were thus found to possess characteristic GPLA complex, which differ from each other and from those of NIH 2 and NIH 13 strain, they should be useful for studies of roles of the major histocompatibility complex in the immune system.

Specific Immune Response Genes of New Inbred Strains of Guinea Pigs

“…The PLL gene, which has been demonstrated to link to MHC of NIH 2 guinea pigs, was found in none of the inbred strains. As one of the Ia specificity of NIH 2 guinea pigs, which was designated as Ia.2b (Chiba et al, 1978) was detected in JY 2 guinea pigs, the presence of the PLL gene in JY 2 guinea pigs was expected but it was not found. This result indicates no association between the PLL gene and Ia.2b detected in JY 2 guinea pigs.…”

“…Since an association between the PLL gene and Ia.2 was recently demonstrated (Shevach et al, 1977), Ia.2b may be distinct from Ia.2. The other Ia specificity, which was recognized by NIH 13 anti-NIH 2 serum and designated as Ia.2a (Chiba et al, 1978), is expected to be Ia.2, since Ia.2a was detected in NIH 2 guinea pigs but not in inbred strains of guinea pigs established in this Institute (Chiba et al, 1978) and the PLL gene being detected in NIH 2 guinea pigs was found in none of the inbred strains as described above.…”

“…In the present communication, we will describe distribution of above-mentioned specific Ir genes in the inbred strains of guinea pigs and discuss the association between specific Ir genes and individual specificities of Ia antigens. On the basis of distribution of Ir genes in the inbred guinea pigs, the identity of Ia specificities detected by us (Chiba et al, 1978) and those designated by Geczy et al (1975) will also be discussed.…”

“…Recently, a detailed study on the MHC of guinea pigs was carried out and three regions, B, S and I, were demonstrated in the MHC (Geczy et al, 1975). More recently, seven I-region associated (Ia) antigens coded for by the I region in the MHC were identified in NIH 2 and NIH 13 guinea pigs and an association between individual Ia specificities and specific Ir genes was demonstrated (Shevach et al, 1977 (Chiba et al, 1978). In the present communication, we will describe distribution of above-mentioned specific Ir genes in the inbred strains of guinea pigs and discuss the association between specific Ir genes and individual specificities of Ia antigens.…”

“…H. Yoda, K. Nakano and M. Nakagawa from random-bred guinea pigs obtained from Rockland Farms, Rockland County, NY., U.S.A. The names and filial generations (F) of the inbred strains used in this study are as follows; antigenic specificities of guinea-pig leukocyte antigens, including Ia antigens, recognized in each strain are shown in parentheses (Chiba et al, 1978); JY 1 (Ia.13a, 13b, P.5), F30--32; JY 2 (Ia.2b, 13b, P.3, P.4, P.6), F31-34; JY 9 (Ia.13a, 13b, P.4, P.5), F40-43; JY 10 (Ia.13a, 13b, P.2, P.3, P.4, P.6), F42-44. In some experiments, random-mated JY 1 guinea pigs obtained from Funabashi Farm, Funabashi, Chiba, were used.…”