Serological Study of Bartonella henselae in Cat Scratch Disease in Japan

Microbiology and Immunology

Sakai

et al. 2002

Seroprevalence of Bartonella henselae was investigated in Japan in 48 individuals clinically suspected of having cat‐scratch disease (CSD), 159 patients with cardiovascular diseases, and 129 healthy veterinary students. Of 48 CSD‐suspected patients examined, 19 (39.6%) were positive for B. henselae‐IgG and 4 (8.3%) for B. henselae‐IgM. Of 159 patients with cardiovascular diseases, 5 (3.1%) were positive for B. henselae‐IgG. In healthy veterinary students, 14 of 129 (10.9%) were positive for B. henselae‐IgG and 1 (0.8%) for B. henselae‐IgM. The positive rates of B. henselae‐IgG and ‐IgM in CSD‐suspected patients were significantly higher than in other sources. Most CSD‐suspected and healthy individuals who were positive for B. henselae antibody had had some contacts with cats. In CSD‐suspected patients, the B. henselae positive rate in females was significantly higher than in males, and high seropositive rates to B. henselae were found in younger age groups.

Section: Discussioncontrasting

confidence: 54%

Serological Investigation ofBartonella henselaeInfections in Clinically Cat‐Scratch Disease‐Suspected Patients, Patients with Cardiovascular Diseases, and Healthy Veterinary Students in Japan

Kikuchi

Microbiology and Immunology

Sakai

et al. 2002

“…Although the climate in Thailand is hot and humid throughout the year, the positive rate of B. henselae in apparently healthy individuals was almost the same as the prevalence in other countries [1,5,8,17,18,[20][21][22]. The exception is a report showing a high incidence (30%) in healthy German individuals [19].…”

mentioning

confidence: 74%

“…The seroprevalence of B. henselae in veterinary professionals was slightly higher than that of other human populations without any special risk factor [12,17]. Furthermore, a higher seroprevalence (12.5%) against B. henselae was reported in healthy individuals with a history of cat exposure in Japan [20] and in suspected CSD patients [18,19,21]. Therefore, these facts may indicate that the seroprevalence against B. henselae in human populations is associated with frequency of contact and/or exposure with cats rather than climate.…”

mentioning

confidence: 99%

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Seroprevalence of Bartonella henselae and Toxoplasma gondii among Healthy Individuals in Thailand.

Boonmar

Morita³

et al. 2000

J. Vet. Med. Sci.

ABSTRACT. The seroprevalence of Bartonella henselae and Toxoplasma gondii among apparently healthy individuals, mainly blood donors, in Thailand was investigated by an indirect fluorescent antibody technique and by a latex agglutination test, respectively. Of 163 serum samples examined, 9 (5.5%) were found to be positive for B. henselae-IgG, 2 (1.2%) for B. henselae-IgM, and 5 (3.1%) for the T. gondii antibody. No significant difference was observed between male and female samples in the serological test with either B. henselae or T. gondii. The age of individuals with B. henselae-IgG was distributed from the 20s to the 70s, and B. henselae-IgM was found in the individuals of the 30s and 60s. The age of T. gondii positive samples ranged from the 20s to the 60s. In this study, the prevalence of B. henselae infection among healthy individuals in Thailand was serologically demonstrated for the first time.-KEY WORDS: antibody, Bartonella henselae, Thailand.

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confidence: 99%

First Isolation of Bartonella henselae Type I from a Cat‐Scratch Disease Patient in Japan and Its Molecular Analysis

Microbiology and Immunology

Izumikawa

Miyashita

et al. 2004

Self Cite

Cat-scratch disease (CSD) is a worldwide zoonosis primarily caused by Bartonella henselae (5,11,20,24,33,34), but Bartonella clarridgeiae has also been linked to a few human cases of CSD (17,19). The organism also causes bacillary angiomatosis (15,31) and peliosis (32) in immunocompromised individuals and bacteremia in immunocompetent individuals. The clinical manifestation of CSD is mainly characterized by pyrexia, papules on the site of the cat scratch or bite and unilateral lymphadenopathy, while cats themselves, the major reservoir of CSD, are asymptotic carriers, showing longterm bacteremia with antibody formation (1, 16).The number of human clinical cases of CSD has been increasing worldwide. Most cases are diagnosed based on clinical manifestations, the history of contacts with cats, serology (14,20,24,26,28,33) or PCR-based methods for examining the biopsy specimens (2, 10, 28). However, the isolation of the organism from patients is relatively difficult and only a few cases of the organism isolation have been reported worldwide (7,9,18,25), because suitable material is not available, the culture conditions are not appropriate or the organism shows fastidious, cytozoic and slow-growth properties. The isolation and establishment of Bartonella strains are extremely important for both etiological diagnosis and epidemiological and genetical analysis of the isolates. Though many clinical cases of CSD have been reported in Japan (20,24,33), there are to date no available reports of the isolation of B. henselae from human cases in Japan.B. henselae type II was detected in 18% of the isolates from CSD patients in the Netherlands (3), and 94% of cat isolates in Germany (27). In contrast, Maruyama et al. showed that type I is the predominant gene type in domestic cats in Japan (21). However, no data was available for the prevalence of gene type on the isolates from CSD patients in Japan.In this study, the authors describe a typical CSD case and the isolation of B. henselae from the patient in Medicine, Yame General Hospital, Fukuoka, Fukuoka 834-0034, Japan Received September 12, 2003; in revised form, October 27, 2003. Accepted November 20, 2003 Abstract: We isolated Bartonella henselae from an inguinal lymph node of a 36-year-old male patient with cat-scratch disease. The patient had many areas of erythema on his body, swelling of the left inguinal lymph nodes with pain and slight fever. The diagnosis was made on the basis of polymerase chain reaction for B. henselae DNA from the lymph node biopsies and blood sample, and isolation of the organism, histology of the lymph node and serology with an indirect immunofluorescent antibody test. We also analyzed the genome profiles for five strains of 90 isolates from the lymph node by pulsed-field gel electrophoresis after NotI endonuclease digestion. We found two different genomic profiles. These results suggest that the patient had been either co-infected or re-infected with two genetically different strains of B. henselae.