Seroprevalence of antibodies to Neospora caniuum and Toxoplasma gondii in water buffaloes (Bubalus bubalis) from Egypt

Dubey, J. P.; Romand, S.; Hilali, M.; Kwok, O. C. H.; Thulliez, P.

doi:10.1016/s0020-7519(97)00190-2

Cited by 68 publications

(43 citation statements)

References 4 publications

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“…Neospora caninum, the etiological agent of bovine neosporosis, is an abortifacient protozoan parasite, implicated in causing infection and subsequent reproductive losses in cattle (2), water buffaloes (3) and other domestic (4) as well as wild animal species (5). Neosporosis in buffalo has been documented from several regions of the world, such as from Brazil (6), Egypt (7), and Thailand (8), and also from Pakistan (9). Economic losses instigated by N. caninum worldwide can be demonstrated as direct costs mediated by abortion (10), together with culling infected animals (11).…”

mentioning

confidence: 99%

Co-infection of water buffaloes in Punjab, Pakistan, with Neospora caninum and Brucella abortus

Nasir¹,

Ashraf²,

Shakoor³

et al. 2014

Turk J Vet Anim Sci

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Neosporosis and brucellosis are 2 well-known diseases causing bovine abortion. This study determined co-infection of Neospora caninum and Brucella abortus in buffaloes for the first time in Pakistan. A monoclonal antibody based competitive ELISA was used to test buffalo sera (n = 312) for N. caninum specific antibodies, while rose bengal plate test assessed B. abortus antibodies. N. caninum and B. abortus prevalence was 42.8% (±6.7, 95% C.I.) and 12.2% (±3.6, 95% C.I.), respectively. Buffaloes >3 years old exhibited higher prevalence than younger ones. Co-infection was particularly significant as 13.2% of Neospora infected buffaloes were also Brucella infected with an overall co-existence of 12.2%, suggesting higher abortion risk in such animals than in those infected with either one of the pathogens. Proper disposal of aborted fetuses and placentae, and testing and culling infected animals are suggested to impede transmission of pathogens.

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mentioning

confidence: 99%

Co-infection of water buffaloes in Punjab, Pakistan, with Neospora caninum and Brucella abortus

Nasir¹,

Ashraf²,

Shakoor³

et al. 2014

Turk J Vet Anim Sci

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“…Antibodies against N. caninum and T. gondii have been show in buffaloes in various countries, nevertheless in Southern Brazil the evidence of these protozoa are still scarce. Seroprevalence studies for N. caninum in buffaloes showed a positivity ranging from 1.5% to 70.9% and are described in southeastern and northern region of Brazil (Fujii et al 2001, Gennari et al 2005, Argentina (Campero et al 2007, Konrad et al 2013, Egypt (Dubey et al 1998), India (Meenakshi et al 2007), Vietnam (Huong et al 1998), and Italy (Guarino et al 2000). Seroprevalence to T. gondii are described in Brazil (Silva et al 2010), Argentina (Konrad et al 2013), Vietnan (Huong et al 1998), Iran (Navidpour & Hoghooghi-Rad 1998), and India (Sharma et al 2008) with the seropositivity ranging from 1.1% to 25.4%.…”

Section: Introductionmentioning

confidence: 97%

“…However, the effects of these diseases in buffaloes are less deleterious than in bovine (Michelizzi et al 2010). Sarcocystis spp., Neospora caninum and Toxoplasma gondii are Apicomplexa protozoa that have worldwide distribution and require an intermediate and a definitive host to complete the life cycle and also have been reported in buffaloes (Dubey et al 1998, Silva et al 2010, Konrad et al 2013. Ruminants can be infected by these parasites through ingesting of the sporocysts or sporulated oocysts present in food or water (Dubey & Lindsay 2006).…”

Section: Introductionmentioning

confidence: 99%

Antibodies against Neospora caninum, Sarcocystis spp. and Toxoplasma gondii detected in buffaloes from Rio Grande do Sul, Brazil

et al. 2016

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ABSTRACT.-Portella L.P., Cadore G.C., Lima M., Sangioni L. The presence of antibodies against Neospora caninum, Sarcocystis spp. and Toxoplasma gondii was evaluated in buffaloes (Bubalus bubalis) from Rio Grande do Sul state (RS), southern Brazil. Serum samples (n=220) were analyzed for antibodies by indirect fluorescent antibody test (IFAT). Antibody presence was considered when the titers were equal or higher than 100 for these protozoa. A total of 60.5% (133/220) buffalo serum samples were positive for at least one of the protozoa evaluated in this study. Antibodies for N. caninum, Sarcocystis spp. and T. gondii were found in 36.4% (80/220), 25.5% (56/220) and 16.8% (37/220) of the buffaloes respectively, indicating a higher frequency of N. caninum infection (p=0.0133). The IFAT is a suitable method to diagnose N. caninum, Sarcocystis spp. and T. gondii infection in buffaloes for detecting IgG antibodies. This study demonstrates the presence of these three protozoa in buffalo herds in RS, Brazil, which may be source of infection to other animals. The high frequency of animals positive for N. caninum is important and could be related to reproductive problems. Additionally, the presence of Sarcocystis spp. and T. gondii in buffaloes can be a possible public health issue.

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“…O primeiro estudo sorológico de N. caninum em búfalos do Egito foi realizado por Dubey et al (1998) que registraram, de 75 exemplares investigados, utilizando o Neospora agglutination test (NAT), a presença de anticorpos contra N. caninun em 68,0% das amostras, nas seguintes diluições: 1:20 em 6 búfalos, 1:40 em 15; 1:160 e 1:320 em 1, e > 1:640 em 28 animais. Na Itália, examinando amostras de soro de 1.377 búfalos (Bubalus bubalis), oriundos de 50 propriedades rurais e utilizando a RIFI para detecção de anticorpos contra N. caninum, Guarino et al (2000) detectaram 34,6% dos animais sororreagentes, observando, ainda, a presença de aborto e sintomas neurológicos em duas das 50 propriedades.…”

Section: Introductionunclassified

“…Navidpour e Hoghooghi-Rad (1998) encontraram 8,8% (34/385) de búfalos com anticorpos contra T. gondii com títulos > 16 pela RIFI, no Irã. Dubey et al (1998), no Egito, encontraram 67,0% (50/75) de fêmeas bubalinas soropositivas para Neospora, a maioria com títulos baixos < 64 no dye test (DT), mas, pelo teste de aglutinação direta modificada (T-MAT), apenas um animal apresentou título de 1:50. Huong et al (1998) obtiveram 3,0% (6/200) de amostras positivas, enquanto em bovinos, 10,5% (21/200), no sul do Vietnã, pelo teste de aglutinação direta; das seis amostras positivas, quatro apresentaram títulos considerados positivos na diluição do soro 1:50 e duas na diluição 1:2.500.…”

Section: Introductionunclassified

Detecção de anticorpos contra Neospora caninum e Toxoplasma gondii em soros de bubalinos ( Bubalus bubalis) no Estado de São Paulo, Brasil

Souza¹,

Nascimento²,

Furuta³

et al. 2001

Sem. Ci. Agr.

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Resumo: Bubalinos são susceptíveis à infecção por Neospora caninum e Toxoplasma gondii, contribuindo assim para a disseminação destes protozoários na natureza. A ocorrência de anticorpos foi investigada em soro sangüíneo de bubalinos, utilizando-se a técnica de imunofluorescência indireta (IFI). Foram avaliados os fatores raça e idade dos búfalos, e também pesquisada a presença de anticorpos contra os dois parasitos em cães e humanos de convívio estreito com os bubalinos. Amostras de soros foram colhidas de búfalos (n=411), cães (n=8) e humanos (n=14), oriundos de propriedades com características leiteiras e examinadas em laboratório. Dentre os soros de bubalinos testados, 56,0% foram reagentes (> 1:200) para N. caninum e 49,9% reagiram (> 1:64) contra T. gondii. Mostraram-se reagentes aos dois protozoários 33,9% dos búfalos. Dos soros de cães 25,0% reagiram ao N. caninum (> 1:50) e 62,5% contra o T. gondii (> 1:16). Nenhum soro humano mostrou-se reagente em níveis considerados positivos (> 100) pela literatura revisada. As fêmeas, entre 5 e 6 anos de idade, apresentaram maior percentual de sororreagentes a T. gondii. Os resultados obtidos permitem inferir que tanto o Neospora caninum quanto o Toxoplasma gondii encontramse bastante disseminados entre os rebanhos investigados e que aparentemente a raça e a idade das fêmeas não interferiram na freqüência de indivíduos sororreagentes ao N. caninum. Palavras-chave: Bubalus bubalis, Neospora caninum, Toxoplasma gondii, RIFI.Abstract: Water buffaloes (Bubalus bubalis), are capable of becoming infected with Neospora caninum and Toxoplasma gondii, contributing to disease dissemination. The incidence of these antibodies was assessed in sera of water buffaloes, using an immunofluorescent antibody technique (IFAT). Any possible influence of breed or age of the buffaloes was investigated, besides assessment of sera antibody titres of any dogs and humans that were in close contact with the tested animals. Serum samples were taken from water buffaloes (n=411), dogs (n=8) and humans (n=14), from buffalo dairy farms in the São Paulo State, and tested. Of the samples of buffalo sera tested, 56.0% were reactive (>1:200) to N. caninum and 49.9% were reactive (>1:64) to T. gondii. Of all, 33.9% of the buffaloes presented antibody titres for both protozoon parasites. Of the canine serum samples, 25.0% presented titres (>1:50) for N. caninum and 62.5% for T. gondii (>1:16). The human serum samples did not show detectable antibodies for N. caninum when using a >100 limit titre for positive findings. The obtained data allows the conclusion that the protozoan parasite N. caninum, as well as T. gondii, are widely disseminated in the water buffalo herds assessed and, apparently, the factors breed and age do not interfere with frequency of N. caninum positives among adult female buffaloes. When concerning T. gondii, female buffaloes of 5 to 6 years of age showed more frequent presence of specific antibodies. Key words: Neospora caninum, Bubalus bubalis, Toxoplasma gondii, Dog, Human, IFAT....

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Seroprevalence of antibodies to Neospora caniuum and Toxoplasma gondii in water buffaloes (Bubalus bubalis) from Egypt

Cited by 68 publications

References 4 publications

Co-infection of water buffaloes in Punjab, Pakistan, with Neospora caninum and Brucella abortus

Co-infection of water buffaloes in Punjab, Pakistan, with Neospora caninum and Brucella abortus

Antibodies against Neospora caninum, Sarcocystis spp. and Toxoplasma gondii detected in buffaloes from Rio Grande do Sul, Brazil

Detecção de anticorpos contra Neospora caninum e Toxoplasma gondii em soros de bubalinos ( Bubalus bubalis) no Estado de São Paulo, Brasil

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