Seroprevalence of Bartonella henselae and Toxoplasma gondii Infections among Pet Cats in Kanagawa and Saitama, Prefectures.

Maruyama, Soichi; Hiraga, Shinya; Yokoyama, Eiji; Naoi, Masayuki; Tsuruoka, Yuji; Ogura, Yoshihiro; Tamura, Katsutoshi; Namba, Shinichi; Kameyama, Yasuhiko; Nakamura, Satoru; Katsube, Yasuji

doi:10.1292/jvms.60.997

Cited by 40 publications

(27 citation statements)

References 24 publications

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“…The antigen slides were made by the same procedure reported previously (10). The frozen sera were thawed at room temperature and treated at 56 C for 30 min to inactivate the complement.…”

Section: Serological Investigation Of Bartonella Henselaementioning

confidence: 99%

Serological Investigation ofBartonella henselaeInfections in Clinically Cat‐Scratch Disease‐Suspected Patients, Patients with Cardiovascular Diseases, and Healthy Veterinary Students in Japan

Kikuchi

Maruyama

Sakai

et al. 2002

Microbiology and Immunology

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Seroprevalence of Bartonella henselae was investigated in Japan in 48 individuals clinically suspected of having cat‐scratch disease (CSD), 159 patients with cardiovascular diseases, and 129 healthy veterinary students. Of 48 CSD‐suspected patients examined, 19 (39.6%) were positive for B. henselae‐IgG and 4 (8.3%) for B. henselae‐IgM. Of 159 patients with cardiovascular diseases, 5 (3.1%) were positive for B. henselae‐IgG. In healthy veterinary students, 14 of 129 (10.9%) were positive for B. henselae‐IgG and 1 (0.8%) for B. henselae‐IgM. The positive rates of B. henselae‐IgG and ‐IgM in CSD‐suspected patients were significantly higher than in other sources. Most CSD‐suspected and healthy individuals who were positive for B. henselae antibody had had some contacts with cats. In CSD‐suspected patients, the B. henselae positive rate in females was significantly higher than in males, and high seropositive rates to B. henselae were found in younger age groups.

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“…The antigen slides were made by the same procedure reported previously (10). The frozen sera were thawed at room temperature and treated at 56 C for 30 min to inactivate the complement.…”

Section: Serological Investigation Of Bartonella Henselaementioning

confidence: 99%

Serological Investigation ofBartonella henselaeInfections in Clinically Cat‐Scratch Disease‐Suspected Patients, Patients with Cardiovascular Diseases, and Healthy Veterinary Students in Japan

Kikuchi

Maruyama

Sakai

et al. 2002

Microbiology and Immunology

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“…Cat scratch disease (CSD) and toxoplasmosis are worldwide zoonoses [3, 6-8, 14, 16, 19-22], and cats are regarded as one of major reservoirs of both diseases [4,11,13,15,21]. Since Thailand is a country of devout Hinayana Buddhism, there are many temples that keep free-living dogs and cats in the precincts.…”

mentioning

confidence: 99%

“…The antigen slides were made by a procedure reported previously [15]. The frozen sera were thawed at room temperature and treated at 56°C for 30 min in order to inactivate them.…”

mentioning

confidence: 99%

Seroprevalence of Bartonella henselae and Toxoplasma gondii among Healthy Individuals in Thailand.

Maruyama

Boonmar

Morita³

et al. 2000

J. Vet. Med. Sci.

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ABSTRACT. The seroprevalence of Bartonella henselae and Toxoplasma gondii among apparently healthy individuals, mainly blood donors, in Thailand was investigated by an indirect fluorescent antibody technique and by a latex agglutination test, respectively. Of 163 serum samples examined, 9 (5.5%) were found to be positive for B. henselae-IgG, 2 (1.2%) for B. henselae-IgM, and 5 (3.1%) for the T. gondii antibody. No significant difference was observed between male and female samples in the serological test with either B. henselae or T. gondii. The age of individuals with B. henselae-IgG was distributed from the 20s to the 70s, and B. henselae-IgM was found in the individuals of the 30s and 60s. The age of T. gondii positive samples ranged from the 20s to the 60s. In this study, the prevalence of B. henselae infection among healthy individuals in Thailand was serologically demonstrated for the first time.-KEY WORDS: antibody, Bartonella henselae, Thailand.

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“…More than three readers evaluated the results subjectively. Plasma samples with a titer of м1:64 were provisionally considered as positive in accordance with the previous reports (19,23).…”

Section: Methodsmentioning

confidence: 69%

“…Titers of IgG antibodies against B. henselae were determined by an indirect immunofluorescence antibody test (IFA) as described previously (19,23). Briefly, plasma was serially diluted with phosphatebuffered saline ranging from 1:32 to 1:1,024 by twofold dilutions and incubated on slides containing Felis catus whole-fetus (FCWF) cells infected with B. henselae Houston-1 at 37 C for 1 hr.…”

Section: Methodsmentioning

confidence: 99%

Predominant T Helper 2 Immune Responses againstBartonella henselaein Naturally Infected Cats

Kabeya

Sase

Yamashita

et al. 2006

Microbiology and Immunology

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Accumulating studies have demonstrated that Bartonella henselae is associated with a spectrum of human diseases including cat-scratch disease (CSD), bacillary angiomatosis, peliosis hepatis, endocarditis, relapsing fever and bacteremia in both immunocompetent and immunocompromised persons (6,18,25,(27)(28)(29). It has been found that bacteremic cats play an important role as a reservoir of B. henselae (5,13,22). Despite high levels of bacteria in cats for many months, most bacteremic cats are asymptomatic, and the pathogenesis and mechanisms of the establishment of persistent infection by B. henselae in cats are not fully understood (9, 17).B. henselae invades feline erythrocytes and endothelial cells, suggesting that the agent belongs to an intracellular pathogen (15). Cell-mediated immunity (CMI) is believed to play significant roles in excluding intracellular pathogens. To evaluate the functions of CMI and humoral immunity (HI), examining the cytokine expression levels in T-lymphocytes is considered important. Because it has not been possible to assess the feline cytokine expression, there has been no information about the effectiveness of CMI against B. henselae infection in cats.In the present study, we used a quantitative reverse transcriptase polymerase chain reaction (RT-PCR) to evaluate the induction of feline gamma-interferon (IFN-γ) and feline interleukin 4 (IL-4), which are representatives of activations of T helper 1 (Th1) and T helper 2 (Th2), respectively. Furthermore, the mRNA expressions of tumor necrosis factor alpha (TNF-α), which is a key cytokine inducing the Th1 responses, were also examined by quantitative RT-PCR (32). The relationship between the levels of bacteremia and the Th1/Th2 cytokines were examined by using three naturally B. henselae infected cats showing relapsing bacteremia. were observed in PBMC from these infected cats after the bacteremia had peaked, showing that the T helper 2 (Th2) responses were specifically induced in the cats. Furthermore, the specific antibody titer increased, resulting in a decrease in the number of B. henselae to undetectable levels in these cats. However, the number of bacteria increased again in two of these cats at 90 and 45 days after the previous bacteremia, respectively. These results suggest that B. henselae predominantly induced IL-4 production from PBMC and resulted in stimulation of the humoral immune responses, including the secretion of specific antibodies in the cats. Furthermore, the specific antibody may play a role in eliminating the bacteria from cats partially but not completely, because relapsing bacteremia was found in these two cats. Predominant T Helper 2 Immune Responses against

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Seroprevalence of Bartonella henselae and Toxoplasma gondii Infections among Pet Cats in Kanagawa and Saitama, Prefectures.

Cited by 40 publications

References 24 publications

Serological Investigation ofBartonella henselaeInfections in Clinically Cat‐Scratch Disease‐Suspected Patients, Patients with Cardiovascular Diseases, and Healthy Veterinary Students in Japan

Serological Investigation ofBartonella henselaeInfections in Clinically Cat‐Scratch Disease‐Suspected Patients, Patients with Cardiovascular Diseases, and Healthy Veterinary Students in Japan

Seroprevalence of Bartonella henselae and Toxoplasma gondii among Healthy Individuals in Thailand.

Predominant T Helper 2 Immune Responses againstBartonella henselaein Naturally Infected Cats

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