Serotype distribution, antimicrobial susceptibility, antimicrobial resistance genes and virulence genes of Salmonella isolated from a pig slaughterhouse in Yangzhou, China

Li, Quan; Yin, Jian; Zheng, Lan; Li, Zewei; Du, Yijun; Guo, Weiwei; Bellefleur, Matthew; Wang, Shifeng; Shi, Huang

doi:10.1186/s13568-019-0936-9

Cited by 30 publications

(16 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here in our study also, we have recovered a few isolates showing the same resistance pattern, which is alarming. Higher level of resistance to Ampicillin and Tetracycline reported by many workers (Li et al 2019) corroborated with the present ndings. Salmonella Enteritidis causes gastroenterological disorders in human and their antimicrobial resistance is a huge concern.…”

Section: Discussionsupporting

confidence: 93%

Serotyping, Antimicrobial Resistance Profile and Virulence Genes of Salmonella Serovars Isolated from Human, Animals and Birds

Borah

Dutta

Das

et al. 2021

Preprint

View full text Add to dashboard Cite

The study was undertaken to investigate the prevalence, antimicrobial susceptibility, antimicrobial resistance and virulence genes of Salmonella isolates recovered from human and different species of animals and birds. Out of 88 (7.15%), 21 (23.86%) belonged to Salmonella enterica subsp. enterica serovar Weltevreden, 22 (25%) to serovar Enteritidis, 16 (18.2%) to serovar Typhi and 14 (15.9%) to serovar Newport, while 7 (7.95%) isolates were found to be untypable. Among the 88 isolates, 45.45% showed resistance to ampicillin, 61.36% to tetracycline, 61.18% to cefotaxime, 65.90% to gentamicin, 48.86% to trimethoprim, 11.36% to ceftriaxone, 10.22% to chloramphenicol, and 7.95% each to ciprofloxacin and cefepime. Most of the isolates were susceptible to a low MIC (≤ 0.25 µg/ml) of Cefepime, Cefotaxime, Ciprofloxacin, Ceftriaxone and Co-trimoxazole and a moderate MIC (0.5µg/ml − 4µg/ml) of Ampicillin, Tetracycline, Gentamicin and Chloramphenicol. The resistance genes, blaTEM, tetA and dfrA12 were most prevalent, irrespective of the host of origin of the isolates. While invA was used for molecular detection of Salmonella, other virulence genes, viz. sipA, sipB, sipC, stn and T2544 were also detected in all (100%) the Salmonella isolates. Total 69.32 % of tested samples were found to be contaminated with multi-drug resistant (MDR) Salmonella and various virulence genes were present among the isolated serovars. Another virulence-associated gene, T2544 (pagN) could also be found in all the isolates, irrespective of serovar or host of origin suggesting the possibility of using this gene as a marker for identification of pathogenic Salmonella isolates. This study highlights the importance of continuous monitoring and surveillance for pathogenic salmonellae and their potential risks to both human and animal.

show abstract

Section: Discussionsupporting

confidence: 93%

Serotyping, Antimicrobial Resistance Profile and Virulence Genes of Salmonella Serovars Isolated from Human, Animals and Birds

Borah

Dutta

Das

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…FIGURE 3 | Heatmap of the detection of antimicrobial resistance genes among the studied Salmonella isolates (n = 78). Zhou et al, 2017) and in Spain (39.7%) (Arguello et al, 2012), while they were higher than those reported in the slaughtered pigs in Sardinia, Italy (12.9%) (Fois et al, 2017); pig carcasses and intestines from five slaughterhouses in Belgium (14.1%) (De Busser et al, 2011); a pig slaughterhouse in Yangzhou, China (17.43%) (Li et al, 2019); pork and slaughterhouse environment in Ahmedabad, Gujarat, India (13.7%) (Chaudhary et al, 2015); and pig slaughterhouses in two different regions of southwestern Spain (12.93%) (Morales-Partera et al, 2018). According to the slaughtering process, samples recovered from the dehairing area and splitting area were the most contaminated samples.…”

Section: Discussionmentioning

confidence: 63%

“…These isolates belong to five different serovars and six MLST patterns, namely, with importance degree, Salmonella Typhimurium ST19, Salmonella Typhimurium ST34, Salmonella London ST155, Salmonella Rissen ST469, Salmonella Goldcoast ST358, and Salmonella Derby ST40. In China, Salmonella Derby was identified as the most isolated serovar from pig slaughterhouse samples (Li et al, 2016(Li et al, , 2019Zhou et al, 2017Zhou et al, , 2018Liu et al, 2020). However, Salmonella Typhimurium has been reported previously as the dominant serovar in Salmonella isolates recovered from pig slaughterhouses in Henan Province (Bai et al, 2015).…”

Section: Discussionmentioning

confidence: 98%

Genomic Investigation of Salmonella Isolates Recovered From a Pig Slaughtering Process in Hangzhou, China

Ed-Dra

Pan

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

The pig industry is the principal source of meat products in China, and the presence of pathogens in pig-borne meat is a crucial threat to public health. Salmonella is the major pathogen associated with pig-borne diseases. However, route surveillance by genomic platforms along the food chain is still limited in China. Here, we conducted a study to evaluate the dynamic prevalence of Salmonella in a pig slaughtering process in Hangzhou, Zhejiang Province, China. Fifty-five of 226 (24.37%) samples were positive for Salmonella; from them, 78 different isolates were selected and subjected to whole genome sequencing followed by bioinformatics analyses to determine serovar distribution, MLST patterns, antimicrobial resistance genes, plasmid replicons, and virulence factors. Moreover, phenotypic antimicrobial resistance was performed using the broth dilution method against 14 antimicrobial agents belonging to 10 antimicrobial classes. Our results showed that samples collected from the dehairing area (66.66%) and the splitting area (57.14%) were the most contaminated. Phenotypic antimicrobial resistance classified 67 of 78 isolates (85.90%) as having multidrug resistance (MDR), while the highest resistance was observed in tetracycline (85.90%; 67/78) followed by ampicillin (84.62%; 66/78), chloramphenicol (71.80%; 56/78), and nalidixic acid (61.54%; 48/78). Additionally, serovar prediction showed the dominance of Salmonella Typhimurium ST19 (51.28%; 40/78) among the 78 studied isolates, while plasmid prediction reported the dominance of IncHI2A_1 (20.51%; 16/78), followed by IncX1_1 (17.95%; 14/78) and IncHI2_1 (11.54%; 9/78). Virulence factor prediction showed the detection of cdtB gene encoding typhoid toxins in two Salmonella Goldcoast ST358 and one Salmonella Typhimurium ST19, while one isolate of Salmonella London ST155 was positive for genes encoding for the siderophore “yersiniabactin” and the gene senB encoding for enterotoxin production. From this study, we conclude that pig slaughterhouses are critical points for the dissemination of virulent and multidrug-resistant Salmonella isolates along the food chain which require the implementation of management systems to control the critical points. Moreover, there is an urgent need for the implementation of the whole genome sequencing platform to monitor the emergence of virulent and multidrug-resistant clones along the food chain.

show abstract

“…It has been shown that Salmonella is widely drug resistant and commonly multidrug resistant [ 66 ], especially with commonly used antibiotics such as tetracycline and trimethoprim. Tetracycline resistance occurs most often by the acquisition of genes that encode efflux pumps such as tet genes [ 67 ].…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and Genotypic Characterization of Virulence Factors and Susceptibility to Antibiotics in Salmonella Infantis Strains Isolated from Chicken Meat: First Findings in Chile

Lapierre

Cornejo

Zavala

et al. 2020

Animals

View full text Add to dashboard Cite

Salmonella Infantis is a zoonotic pathogen that causes gastroenteritis in humans and animals, with poultry being its main reservoir. In Chile, there are no data to characterize S. Infantis strains in poultry production. In this study, 87 S. Infantis strains were isolated from chicken meat for sale in supermarkets in Santiago, Chile, and characterized according to their virulence genes, biofilm formation abilities, antibiotic susceptibility, and resistance genes. Through polymerase chain reaction or PCR, the strains were analyzed to detect the presence of 11 virulence genes, 12 antibiotic resistance genes, and integrase genes. Moreover, disc diffusion susceptibility to 18 antimicrobials and the ability to form biofilm in vitro were evaluated. Results demonstrated six different virulence gene profiles. Ninety-four percent of the strains were multi-resistant to antibiotics with weak biofilm formation abilities, 63.2% of the strains were broad spectrum β- lactam resistant, and the bla CTX-M-65 gene was amplified in 13 strains. Only 3.4% of the strains were fluoroquinolone resistant, and the qnrB gene was amplified in two strains. Colistin resistance was exhibited in 28.7% of the strains, but mrc genes were not amplified in any strain under study. The isolated S. Infantis strains are pathogenic and antibiotic multi-resistant, and thus, this Salmonella serotype should be under surveillance in the poultry food production chain with the aim of protecting public health.

show abstract

Serotype distribution, antimicrobial susceptibility, antimicrobial resistance genes and virulence genes of Salmonella isolated from a pig slaughterhouse in Yangzhou, China

Cited by 30 publications

References 44 publications

Serotyping, Antimicrobial Resistance Profile and Virulence Genes of Salmonella Serovars Isolated from Human, Animals and Birds

Serotyping, Antimicrobial Resistance Profile and Virulence Genes of Salmonella Serovars Isolated from Human, Animals and Birds

Genomic Investigation of Salmonella Isolates Recovered From a Pig Slaughtering Process in Hangzhou, China

Phenotypic and Genotypic Characterization of Virulence Factors and Susceptibility to Antibiotics in Salmonella Infantis Strains Isolated from Chicken Meat: First Findings in Chile

Contact Info

Product

Resources

About