Serotyping and antimicrobial susceptibility of Salmonella isolated from children under five years of age with diarrhea in rural Burkina Faso

DembÃ©lÃ©, RenÃ©; KonatÃ, Ali; Bonkoungou, Isidore Juste O.; ̈ga, AssÃ ̈ta KagambÃ; KonatÃ, Kiessoun; BagrÃ, Touwendsida Serge; TraorÃ, Alfred S.; Barro, Nicolas

doi:10.5897/ajmr2014.7002

Cited by 10 publications

(2 citation statements)

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“…In 2019, pathogens responsible for foodborne diseases, including Salmonella enterica (non-typhoid), caused 230,000 deaths in Africa [ 7 ]. In resource-limited countries, particularly in Sub-saharan Africa, NTS is endemic, with high rates in children under 3 years of age and immunocompromised individuals [ 8 , 9 ]. These diseases have a significant negative economic impact in resource-limited countries amounting to $ 110 billion per year [ 10 ].…”

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confidence: 99%

Characterization of virulence factors of Salmonella isolated from human stools and street food in urban areas of Burkina Faso

et al. 2021

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Background This study was undertaken to identify and functionally characterize virulence genes from Salmonella isolates in street food and stool cultures. From February 2017 to May 2018, clinical and food Salmonella strains were isolated in three regions in Burkina Faso. Salmonella was serotyped according to the White-Kauffmann-Le Minor method, and polymerase chain reaction (PCR) was used to detec invA, spvR, spvC, fimA and stn virulence genes commonly associated with salmonellosis in Sub-Saharan Africa. Results A total of 106 Salmonella isolates (77 human stools; 14 sandwiches) was analyzed using a serological identification with an O-group test reagent. The presence of Salmonella was confirmed in 86% (91/106) of the samples were reactive (OMA-positive/OMB-positive). Salmonella serogroup O:4,5 was the most common serogroup detected (40%; 36/91). Salmonella Enteritidis and Typhimurium represented 5.5% (5/91) and 3.3% (3/91), respectively and were identified only from clinical isolates. Furthermore, 14 serotypes of Salmonella (12/91 human strains and 2/15 sandwich strains) were evocative of Kentucky/Bargny serotype. For the genetic profile, 66% (70/106) of the Salmonella had invA and stn genes; 77.4% (82/106) had the fimA gene. The spvR gene was found in 36.8% (39/106) of the isolates while 48.1% (51/106) had the spvC gene. Among the identified Salmonella Enteritidis and Salmonella Typhimurium isolated from stools, the virulence genes detected were invA (3/5) versus (2/3), fimA (4/5) versus (3/3), stn (3/5) versus (2/3), spvR (4/5) versus (2/3) and spvC (3/5) versus (2/3), respectively. Conclusion This study reports the prevalence of Salmonella serotypes and virulence genes in clinical isolates and in street foods. It shows that food could be a significant source of Salmonella transmission to humans. Our results could help decision-making by the Burkina Faso health authority in the fight against street food-related diseases, in particular by training restaurateurs in food hygiene.

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Section: Introductionmentioning

confidence: 99%

Characterization of virulence factors of Salmonella isolated from human stools and street food in urban areas of Burkina Faso

et al. 2021

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“…Limited number of studies has been carried out on Salmonella serotyping and its susceptibility to antibiotics in urban and rural setting in Burkina Faso (Bonkoungou et al, 2013;Dembélé et al, 2014). There are no data on the prevalence of resistance bacterial genes among rural communities in the country.…”

Section: Introductionmentioning

confidence: 99%

Involvement of class 1 and class 2 integrons in dissemination of tet and catA1 resistance genes of Salmonella enterica from children with diarrhea in rural Burkina Faso

DembÃ©lÃ©¹,

KaborÃ©²,

Soulama³

et al. 2020

Afr. J. Biotechnol.

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With high annual mortality rates among young children, antimicrobial resistant salmonellosis is considered a major public health concern worldwide. Antimicrobial resistant salmonellosis is a worldwide health issue, particularly in low income countries with high microbially-derived food contaminations. As a result, it is important to better understand the biological factors that may control these bacteria's dissemination low immunity individuals such as children. Thus, a sound epidemiological surveillance and control of salmonellosis (that is, tet and catA1) requires a better understanding of the role that class 1, 2 and 3 integrons play in the spread of these antimicrobial resistant genes. A total of 275 stool samples of children suffering of diarrhea in rural Burkina Faso were collected and their Salmonella species were screened. The antimicrobial resistance determinants were investigated by Polymerase Chain Reaction, checking the presence of class 1, 2, 3 integrons, tet and catA1 resistance genes. Seven of the nine confirmed Salmonella strains (78%) were multidrug resistant while 100% were resistant to amoxicillin. Antibiotic resistance genes catA and tet were present in 11.1 and 22.2%, respectively. Integrons were detected as follows: Int1 (44.4%) and Int2 (22.2%). No class 3 integron was detected. A surveillance and control programme of antimicrobial drug resistant Salmonella species is of paramount importance for limiting spread of these pathogens among children.

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Diversity and antimicrobial drug resistance of non-typhoid Salmonella serotypes isolated in lettuce, irrigation water and clinical samples in Burkina Faso

Somda

Bonkoungou²,

Sambe-Ba

et al. 2021

Journal of Agriculture and Food Research

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Serotyping and antimicrobial susceptibility of Salmonella isolated from children under five years of age with diarrhea in rural Burkina Faso

Cited by 10 publications

References 2 publications

Characterization of virulence factors of Salmonella isolated from human stools and street food in urban areas of Burkina Faso

Characterization of virulence factors of Salmonella isolated from human stools and street food in urban areas of Burkina Faso

Involvement of class 1 and class 2 integrons in dissemination of tet and catA1 resistance genes of Salmonella enterica from children with diarrhea in rural Burkina Faso

Diversity and antimicrobial drug resistance of non-typhoid Salmonella serotypes isolated in lettuce, irrigation water and clinical samples in Burkina Faso

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